LOCUS       sequence-224562-       10037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: GRP94-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..10037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3571..4356
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4363..4428
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4435..4452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4488..4543
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4542..4561)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6779..6797)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6865..6969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6970..7038,7039..7830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8001..8589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8490..8509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8743..8760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8877..8898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8913..8918,8919..8936,8937..8943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8951..8967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8956..8978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8975..8991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8975..8991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9262..9641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9642..9845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9796..9816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9842..9866
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccgat
      421 agcagcgtgg aaggcgataa gggccccagc gacgacgtgt ccgacagcag cggcgagaag
      481 aaagaagtga agcgcgaccg ggacaccctg gaagagatcg aggaaggcga gaagcccacc
      541 gagagcatgg aaagccacca gtaccagacc gaagtgaccc ggctgatgga catcatcgtg
      601 aacagcctgt atacccagaa agaggtgttc ctgagagagc tgatctccaa cgccgccgac
      661 gccctggaaa agattcggtt cctgagcctg agcgacgaga gcgtgctggg cgaggaaaag
      721 aagctggaaa tccggatcag cgccaacaaa gagaagaaca tcctgagcat caccgacacc
      781 ggcatcggca tgaccaaggt ggacctgatc aacaacctgg gcacaatcgc caagagcggc
      841 accagcaact ttctggaagc catcagcaag tctggcggcg acatgagcct gatcggccag
      901 ttcggcgtgg gcttttacag cgccttcctg gtggccgaca aagtgatcgt gtacaccaag
      961 aacaacgacg acgagcagta catctgggag agcaccgccg atgccaagtt caccatctac
     1021 aaggacccca gaggcgccac cctgaagcgg ggcaccagaa tcagcctgca cctgaaagag
     1081 gacgccacca acctgctgaa cgacaagaaa ctgatggatc tcatctccaa gtacagccag
     1141 ttcatccagt tccctatcta cctgctgcac gagaatgtgt acaccgagga agtgctggcc
     1201 gatatcgcca aggacatggt caacgacccc aactacgaca gcgtgaaggt ggaagagaca
     1261 gacgacccta acaagaaaac ccggaccgtg gaaaagaaag tgaagaaatg gaccctgatg
     1321 aacgagcagc ggcccatctg gctgcggagc cccaaagagc tgaaggacga ggactacaag
     1381 cagttcttca gcgtgctgag cggctacaac gaccagcccc tgtaccacat ccactttttc
     1441 gccgagggcg agatcgagtt caagtgcctg atctacatcc ccagcaaggc ccctagcatg
     1501 aacgatcagc tgtactccaa gcagaactcc ctgaagctgt acgtgcggcg ggtgctggtg
     1561 gctgacgagt tcgtggaatt cctgccccgg tacatgagct tcgtgaaggg cgtggtggac
     1621 agcgacgatc tgcccctgaa cgtggccaga gagcagctgc agcagaacaa gatcctgaag
     1681 gccgtgtcca agcggatcgt gcggaagatc ctggacacct tccacaagct gtacaaagag
     1741 ggcaagaaga acaaagaaac cctgcggagc gagctggaaa acgaggccga cgaggaaaaa
     1801 aagaaagaga tcaccaagaa gctgagcgag cccagcacct acaagctcat ctacaaagag
     1861 taccggaagt tcctgaagtc cggctgctac gaggacgaca tcaacagaaa caagatcgcc
     1921 aagctgctgc tgttcaagac catgcagtac cccaagagca tcagcctgga tacctacatc
     1981 gagcacatga agcccgacca gaagttcatc tactacgcca gcggcgacag ctacgagtac
     2041 ctggccaaga tccctcagct gcagatcttc aagaagaaga atatcgacgt cctgttcctg
     2101 accgagtccg tggacgagag ctgcatccag cgggtgcagg aatacgaggg aaagaagttc
     2161 aagtccatcc agaagggcga aatcagcttc gagctgacag aggaagagaa aaaaaaggaa
     2221 cagcagatgc agaagatgta caaggccctg atcgacgtga tctccgatac cctgaagaac
     2281 cagatcttta aggtggaaat cagcagacgg ctggtggacg ccccttgcgc cgtggtgtct
     2341 acagagtggg gcctgtccgg ccagatggaa aagctgatga agatgaatgt ggccaacagc
     2401 gaccagatca aggccatgag cggccagaag attctggaaa tcaaccccaa ccaccccatc
     2461 atgatcgatc tgctgaagcg gagcgtgacc aaccccaagg acctggaact gaccaacagc
     2521 atcaagatca tgtaccagag cgccaaactg gccagcggct tcgacctgga agataccgcc
     2581 gacctggccc agattgtgta cgaccacatt aaccagaaac tgggcgtgga caacaacctc
     2641 aagatcgacg acctggaccc cagcatcttc gagacaaaga agattgagga cgagaacgac
     2701 gccagcaagt tcgaggaaga aatcaacatt gacgacgaga tccagaagaa ggataacaac
     2761 gtcgacaacg aggccaatga caagtccgac gaactcggcg cgccgtcgac ctccatcacg
     2821 gcctataaga gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa
     2881 agcctgcagg gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc
     2941 accttctccc taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc
     3001 cagctgtccg aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct
     3061 ggttctggca acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg
     3121 gtggtgatga aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc
     3181 acctcaccca agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg
     3241 caggagaaag tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt
     3301 gaaggtgaag aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc
     3361 ggcggcggct ccggaggcgg taccggtggc ggcgacctag ccccacagat gcttcgagaa
     3421 ctccaggaga ctaatgcggc gctgcaagac gtgagagagc tcttgcgaca gcaggtcaag
     3481 gagatcacct tcctgaagaa tacggtgatg gaatgtgacg cctgcggtct agacagaaac
     3541 ctgcctccac tagcacctct gggcccacac ccagaaacgc tggtgaaagt aaaagatgct
     3601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4021 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4321 atcgctgaga taggtgcctc actgattaag cattggggcc ctgactacaa agaccatgac
     4381 ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg ccctcaccac
     4441 caccatcacc actaataata aggatccccc gacctcgacc tctggctaat aaaggaaatt
     4501 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     4561 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     4621 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     4681 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     4741 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     4801 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     4861 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     4921 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     4981 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     5041 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     5101 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     5161 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     5221 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     5281 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     5341 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     5401 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     5461 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     5521 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     5581 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     5641 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     5701 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     5761 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     5821 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     5881 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     5941 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     6001 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     6061 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     6121 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     6181 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     6241 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     6301 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     6361 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     6421 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     6481 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     6541 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     6601 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     6661 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     6721 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     6781 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7201 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7501 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8701 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8761 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8821 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8881 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8941 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9001 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     9061 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     9121 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     9181 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     9241 gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
     9301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     9361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     9421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     9481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     9541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     9601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     9661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     9721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     9781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     9841 gagctcgttt agtgaaccgt cagatcctca ctctcttccg catcgctgtc tgcgagggcc
     9901 agctgttggg ctcgcggttg aggacaaact cttcgcggtc tttccagtac tcttggatcg
     9961 gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg acctgagcga gtccgcatcg
    10021 accggatcgg aaaacct
//
