LOCUS       sequence-224564-       16139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeted DNA methylation tool.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16139)
  AUTHORS   Su J, Huang YH, Cui X, Wang X, Zhang X, Lei Y, Xu J, Lin X, Chen K, 
            Lv J, Goodell MA, Li W
  TITLE     Homeobox oncogene activation by pan-cancer DNA hypermethylation.
  JOURNAL   Genome Biol. 2018 Aug 10;19(1):108. doi: 10.1186/s13059-018-1492-3.
  PUBMED    30097071
REFERENCE   2  (bases 1 to 16139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-08-10"; volume: "19(1):108. doi"; pages: " 
            10.1186/s13059-018-1492-3"
COMMENT     Sequence Label: Pinducer20-dCas9-SunTag-2A-BFP
FEATURES             Location/Qualifiers
     source          1..16139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2737..2757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      2806..2815
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2812..2838,2839..2841,2842..5328,5329..5331,
                     5332..6915)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2812..6915
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6919..6945
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6925..6951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             6964..6984
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6991..7011
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7075..7131
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7147..7203
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7219..7275
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7291..7347
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7363..7419
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7435..7491
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7507..7563
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7579..7635
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7651..7707
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7723..7779
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7861..7881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7891..7947
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7960..8658
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             8668..8688
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(8711..8735)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8788..8814
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        8900..10111
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9948..9967
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(10170..10787,10788..10793,10794..10829,10830..10832,
                     10833..10868,10869..10871,10872..10907,10908..10916)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    10969..11542
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11136..11153)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11363..11382
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11558..12352
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11558..12352
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11612..11631)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12222..12241
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    12529..13117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12582..12602)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13000..13011)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13192..13425
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(13544..13562)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13630..13734
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13735..13803,13804..14595)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13953..13972)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14766..15354
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15255..15274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15508..15525
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15765..15786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15801..15806,15807..15824,15825..15831)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15839..15855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15844..15866
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15863..15879
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15863..15879
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15929..15948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctccgcc accatgccca agaagaagcg caaggtggga
     2761 cgcgtctgca ggatatcaag cttgcggtac cgcgggcccg ggatcgccac catggacaag
     2821 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
     2881 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
     2941 aagaagaacc tgatcggcgc cctgctgttc gacagcggag aaacagccga ggccacccgg
     3001 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
     3061 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
     3121 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
     3181 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
     3241 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     3301 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     3361 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
     3421 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
     3481 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa cctgattgcc
     3541 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     3601 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     3661 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     3721 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag
     3781 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     3841 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatc
     3901 gatggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     3961 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg
     4021 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg
     4081 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     4141 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     4201 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     4261 aagggcgcca gcgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     4321 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtacaacgag
     4381 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag
     4441 cagaaaaaag ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     4501 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg
     4561 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
     4621 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     4681 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
     4741 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
     4801 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     4861 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     4921 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     4981 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     5041 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg
     5101 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     5161 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     5221 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     5281 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct
     5341 cagagctttc tgaaggacga ctccatcgat aacaaagtgc tgactcggag cgacaagaac
     5401 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     5461 cgccagctgc tgaatgccaa gctgattacc cagaggaagt tcgacaatct gaccaaggcc
     5521 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     5581 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     5641 gacgagaacg acaaactgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     5701 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     5761 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     5821 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     5881 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     5941 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     6001 gagacaaacg gcgaaacagg cgagatcgtg tgggataagg gccgggactt tgccaccgtg
     6061 cggaaagtgc tgtctatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     6121 ggcttcagca aagagtctat cctgcccaag aggaacagcg acaagctgat cgccagaaag
     6181 aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg
     6241 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     6301 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa
     6361 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     6421 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga
     6481 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     6541 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaaa
     6601 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     6661 gacgctaatc tggacaaggt gctgagcgcc tacaacaagc acagagacaa gcctatcaga
     6721 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc
     6781 ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg
     6841 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     6901 cagctgggag gcgacgccta tccctatgac gtgcccgatt atgccagcct gggcagcggc
     6961 tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt ggacggcatt
     7021 ggtagtggga gcaacggcag cagcggatcc aacggtccga ctgacgccgc ggaagaagaa
     7081 cttttgagca agaattatca tcttgagaac gaagtggctc gtcttaagaa aggttctggc
     7141 agtggagaag aactgctttc aaagaattac cacctggaaa atgaggtagc tagactgaaa
     7201 aaggggagcg gaagtgggga ggagttgctg agcaaaaatt atcatttgga gaacgaagta
     7261 gcacgactaa agaaagggtc cggatcgggt gaggagttac tctcgaaaaa ttatcatctc
     7321 gaaaacgaag tggctcggct aaaaaagggc agtggttctg gagaagagct attatctaaa
     7381 aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagg gaagtggcag tggtgaagag
     7441 ctactatcca agaattatca tcttgagaac gaggtagcgc gtttgaagaa gggttccggc
     7501 tcaggagagg aactgctctc gaagaactat catcttgaaa atgaggtcgc tcgattaaaa
     7561 aagggatcgg gcagtggtga ggaactactt tcaaagaatt accacctcga aaacgaagta
     7621 gctcgattaa agaaaggttc agggtcgggt gaagaattac tgagtaaaaa ttatcatctg
     7681 gaaaatgagg tagcgagact aaaaaagggg agtggttctg gcgaggaatt gctatcgaaa
     7741 aattatcatc ttgagaacga agttgctagg ctcaaaaagg gctcaggctc aggcaccgcg
     7801 gtaaacatag gtggtggaac cggtccgatg gatctacagc ggccgcaagg tggaggtgga
     7861 cccaagaaga agcgcaaggt gggaagcgga gctactaact tcagcctgct gaagcaggct
     7921 ggagacgtgg aggagaaccc tggacctgca ccaggaagta tgagcgagct gattaaggag
     7981 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
     8041 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     8101 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
     8161 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
     8221 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     8281 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
     8341 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
     8401 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
     8461 agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc
     8521 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
     8581 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
     8641 ctggggcaca agcttaatgg tggaggtcca aaaaagaaaa ggaaggtcta acctgcaggg
     8701 cttatcgatg acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
     8761 tcgagtctag agggcccgcg gttcgaatac ccatacgacg tcccagacta cgcttagtaa
     8821 tgattaatta aactagaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     8881 agtaacccac ccaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
     8941 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc
     9001 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat
     9061 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca
     9121 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg
     9181 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg
     9241 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg
     9301 agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg
     9361 aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa
     9421 ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg
     9481 cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa
     9541 cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg
     9601 ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt
     9661 ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag
     9721 cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg
     9781 ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct
     9841 agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag
     9901 gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt
     9961 ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga
    10021 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
    10081 attctggccg tttttggctt ttttgttaga cgaagcttgg taccgagctc ggatctccac
    10141 cccgtaccgg tcctgcagtc gaattcacca tgtctagact ggacaagagc aaagtcataa
    10201 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
    10261 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
    10321 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
    10381 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
    10441 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
    10501 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
    10561 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
    10621 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
    10681 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
    10741 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
    10801 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
    10861 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
    10921 gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc cctctccctc
    10981 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
    11041 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
    11101 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
    11161 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
    11221 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
    11281 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
    11341 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
    11401 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
    11461 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
    11521 cctttgaaaa acacgatgat aagcttaccg gtccaccatg attgaacaag atggattgca
    11581 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
    11641 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
    11701 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
    11761 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
    11821 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
    11881 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
    11941 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
    12001 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
    12061 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
    12121 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
    12181 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
    12241 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
    12301 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gatgtacaag
    12361 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
    12421 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    12481 ttgtttagtc cctcccaatt cgatatcaag cttatcgcga tagatcctaa tcaacctctg
    12541 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    12601 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    12661 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    12721 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    12781 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    12841 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    12901 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    12961 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    13021 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    13081 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
    13141 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    13201 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    13261 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    13321 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    13381 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
    13441 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
    13501 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
    13561 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    13621 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    13681 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    13741 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    13801 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    13861 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    13921 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
    13981 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    14041 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    14101 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    14161 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    14221 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    14281 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    14341 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    14401 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    14461 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    14521 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    14581 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    14641 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    14701 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    14761 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    14821 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    14881 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    14941 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    15001 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    15061 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    15121 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    15181 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    15241 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    15301 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    15361 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    15421 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    15481 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    15541 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
    15601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    15661 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
    15721 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    15781 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    15841 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    15901 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    15961 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    16021 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    16081 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
