LOCUS       sequence-224570-        5553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red fluorescent voltage indicator for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Kannan M, Vasan G, Huang C, Haziza S, Li JZ, Inan H, Schnitzer MJ, 
            Pieribone VA
  TITLE     Fast, in vivo voltage imaging using a red fluorescent indicator.
  JOURNAL   Nat Methods. 2018 Dec;15(12):1108-1116. doi: 
            10.1038/s41592-018-0188-7. Epub 2018 Nov 12.
  PUBMED    30420685
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0188-7"; journalName: "Nat Methods"; date: 
            "2018-12"; volume: "15"; issue: "12"; pages: "1108-1116"
COMMENT     Sequence Label: CMV-VARNAM
FEATURES             Location/Qualifiers
     source          1..5553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..1005
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     primer_bind     complement(2574..2593)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2639..2694
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2693..2712)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3055..3071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3055..3071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3068..3090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3079..3095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3103..3109,3110..3127,3128..3133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3148..3169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3228..3423
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3274..3409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3429..3563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3466..3485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3520..3539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3631..3648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3802..4390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3882..3901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4561..5352,5353..5421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5184..5203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5422..5526)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgactgca gagggccctc gaccatagcc aattcaatat ggcgtatatg gactcgtgcc
       61 aattcaatat ggtggatctg gaccccagcc aattcaatat ggcggaywtg gmmycrtgcc
      121 aattcaatat ggyggatctg gaccccagcc aattcaatat ggcggacttg gcaccatgcc
      181 aattcaatat ggcggacctg gcactgtgcc aactggggag gggtctactt ggcacggtgc
      241 caagtttgag gaggggtctt ggccctgtgc caagtccgcc atattgaatt ggcatggtgc
      301 caataatggc ggccatattg gctatatgcc aggatcaata tataggcaat atccaatatg
      361 gccctatgcc aatatggcta ttggccaggt tcaatactat gtattggccc tatgccatat
      421 agtattccat atatgggttt tcctattgac gtagatagcc cctcccaatg ggcggtccca
      481 tataccatat atggggcttc ctaataccgc ccatagccac tcccccattg acgtcaatgg
      541 tctctatata tggtctttcc tattgacgtc atatgggcgg tcctattgac gtatatggcg
      601 cctcccccat tgacgtcaat tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc
      661 aataggacca cccaccattg acgtcaatgg gatggctcat tgcccattca tatccgttct
      721 cacgccccct attgacgtca atgacggtaa atggcccact tggcagtaca tcaatatcta
      781 ttaatagtaa cttggcaagt acattactat tggaagtacg ccagggtaca ttggcagtac
      841 tcccattgac gtcaatggcg gtaaatggcc cgcgatggct gccaagtaca tccccattga
      901 cgtcaatggg gaggggcaat gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg
      961 cgtgcctaat gggaggtcta tataagcaat gctcgtttag ggaacggtac caccatggct
     1021 gacgtggaaa ccgagaccgg catgattgca cagtggattg tctttgctat tatggctgct
     1081 gctgctattg cttttggagt ggctgtgcac tttcggcctt cagagctgaa gagcgcatac
     1141 tatatcaaca ttgccatctg cactatcgcc gctaccgctt actatgcaat ggccgtgaac
     1201 taccaggacc tgacaatgaa tggtgaaagg caggtggtct acgcaagata tattagctgg
     1261 gtgctgacca caccactgct cctgctcgat ctcatcgtca tgaccaagat gggcggagtg
     1321 atgatttctt gggtcatcgg cgcagacatt ttcatgatcg tgtttggtat tctgggcgcc
     1381 ttcgaggatg aacacaagtt caaatgggtg tactttatcg ctggatgtgt gatgcaggca
     1441 gtcctgacat acgggatgta taacgccact tggaaagacg atctgaagaa aagccccgag
     1501 taccatagct cctatgtcag tctgctcgtc ttcctgtcaa tcctctgggt gttttatcct
     1561 gtcgtgtggg ctttcgggtc tggtagtggc gtgctgtccg tcgacaatga ggccattctc
     1621 atgggaatcc tggatgtgct cgctaagcca ctgtttggaa tggggtgcct cattgcccat
     1681 gagactatct tcaagtggag gtccggactg attaaggaaa atatgcggat gaaggtcgtg
     1741 atggaagggt ctgtcaatgg gcaccagttc aagtgcaccg gagagggaga gggcaggcca
     1801 tacgagggcg tgcagacaat gaggatcaaa gtgatcgagg gaggaccact gcctttcgcc
     1861 tttgacatcc tggccaccag cttcatgtac ggcagcagga ccttcatcaa gtatccagcc
     1921 gacatccccg atttctttaa gcagagcttc cccgagggct ttacctggga gagggtgaca
     1981 agatacgagg atggcggcgt ggtgaccgtg acacaggaca cctctctgga ggatggcgag
     2041 ctggtgtata acgtgaaggt gaggggcgtg aacttcccta gcaatggccc agtgatgcag
     2101 aagaagacca agggctggga gcccaataca gagatgatgt accctgcaga cggaggcctg
     2161 aggggatata ccgacatcgc cctgaaggtg gatggaggag gacacctgca ctgtaacttt
     2221 gtgaccacat accgctccaa gaagacagtg ggcaatatca agatgccagg agtgcacgcc
     2281 gtggaccaca ggctggagcg catcgaggag tctgataacg agacatatgt ggtgcagaga
     2341 gaggtggccg tggccaagta ctctaatctg ggcggcggga tggacgagct gtataagaag
     2401 agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat caacgtgttc
     2461 tgctacgaga acgaggtgta atgagctagc ggccgcactc ctcaggtgca ggctgcctat
     2521 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     2581 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     2641 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     2701 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     2761 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     2821 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     2881 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     2941 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     3001 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     3061 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3181 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag
     3241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3421 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3481 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3541 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
     3601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5521 tccgcgcaca tttccccgaa aagtgccacc tgg
//
