LOCUS       sequence-224595-       10502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: B9-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..10502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4036..4821
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4828..4893
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4900..4917
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4953..5008
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5007..5026)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7244..7262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7330..7434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7435..7503,7504..8295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8466..9054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8955..8974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9208..9225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9342..9363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9378..9383,9384..9401,9402..9408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9416..9432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9421..9443
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9440..9456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9440..9456
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9727..10106
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10107..10310
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10261..10281
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10307..10331
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccggc
      421 gaggaaaagc tgaccaagtt cgaccccgtg atgatcaaag agggcgagct gttcggcgag
      481 gtgtcctacg actgcgagta cagcggcttc ctggaaatcg agaacgacga tatcctgtac
      541 tactgcttct tcgtgggcga ggacgaccac aacggcctgc tgctgcggaa gttccacatg
      601 gacgacatgg tctggggcat ccccatggaa gtgctgatca cccggaagaa gatcaacctg
      661 tacatcctga ccgtggacta catcctgtcc aagctgatcc tgatctacag cttcgacgag
      721 aagatccgca tcaccgtgtt caacaactac atggaagagt ccgtgatgag cgagaagatt
      781 agcatcaact acgagatcct ctacaaggcc aacatgatca gctacatcac ctacttctac
      841 aagaacaaga agtccctctg cgtctgcggc atgaaccgga acgagaacat cctgtgcacc
      901 ttcagcttcg attacggcct gaccatgaag gacgatcaca ccatcgagtt ctttctgaag
      961 aagaagattc ccctgggcca ctacaagatg caggtcacat tcaaagaaac cgaggtgtac
     1021 ttcaacctct acaacgacct gaacgaggcc aacttctacg agctgaagtg cgtgaagaaa
     1081 caggacgagt acatctgcga catcatgaac aagatccggg aaacccagga gtacaactac
     1141 aagcggtaca cctaccccag cagcgagcac aacaagtact acaacgatca ccacaacaag
     1201 cagccccagg gcaagcaggg acagcagggc ggagcccctc tgcccaacct gggagaggaa
     1261 gagaacaact tcatctacaa gcacatcgtg cggaacaagt atttccagat gatcgtgttc
     1321 cagaaagacc agaagtgcta cctggcctgg tccttcaaca gcctgaacat caacgatgag
     1381 atcaccaaag aaatcagcag cgtgccctgc agcaacgtgg ccagctacta ccggcaggaa
     1441 cggctgatcg tcaccctgaa gaaaaaccag ctgggcagcc ggaaccactt ttttctgatc
     1501 tacgaaaagc tcaccgagaa gggcgtgggc tgcgagtttg gcgtgggcgg cagcctgtac
     1561 gtgaccaaga cctttatgaa caacgcctgc gacatggaca tcaacaacat gaatgtggcc
     1621 accaccaact acgacgagat caacttcgcc ctgatcatcc ctctgagctt ccagctgaac
     1681 tacgccacct gtttcatctg ggaggaaaac gtggacaaca acgaggaacg gtccagcctg
     1741 tattacctgg aagagtacac caacgagaaa gaggacatca aggtctacac cttctacttt
     1801 tacaagtaca tcctcatcta taagaacttt aagaagagta cctgcacctt cgagagcaag
     1861 aacaacgaga agctgtacat ctccttccgg ggcgacacct actacaaaga gtacaattgc
     1921 aacatcctgc tggacagctg cgacttcttc ctgcacaccc agagcaagat caatatcgcc
     1981 tacgacgacg acgtgtggca ggtcagcgag gaactgtacg acggcttcgt gatgtacaac
     2041 ggcgcctacg tgtccctgta tgacgtgctg agcagaagca acgcccacgg cctgatctat
     2101 atcgacggca aagaaaacaa cgccgccatc aacctgaaca acattaagaa caacaacaac
     2161 aacaataaca ataacaacaa taataacaac gccgccaaca acaacaatat cgccaacaac
     2221 gccaactcca acaatgcctt cggcgagcac gagaaagtga tcaccatcat catcccttac
     2281 ggcatcccta gcacccggac catcaagatc gagtttctga acaagcagaa caatgaagag
     2341 aagcgctggg cctacctgcg gctgcagaaa aacgagtata tcatgaagaa agtcatcggc
     2401 atcaacagcg ccgacatctt cgacctgagc tacaagtatt ataagtacaa ccaggaaaag
     2461 atcaagttca tcctgaacga cttcagcgag acaacctacc tgggcttcat ctgccagacc
     2521 aaagaagaga tcaagaaaag cctctgcacc atcagcctgg tggaccacag ccacaagaac
     2581 atggccatta acaacctgtt caagaccaac aaccacatcc tgccctttct gtacgaccag
     2641 agccctcccc ggaagatcca gccctacaac cagatcatca gcgagttcag attcgtcctc
     2701 ttcaagaact tccatctgtt tctgcaggaa aacaagatca actatatcct gctcaagtgc
     2761 atgtgcagca gctccagctc ctacatcaac acccagaaga acatcgagct gtcctacctg
     2821 atcaccaacg aacaggtgtc ccacgatttc atccacaccc ctggcatcct gatccggccc
     2881 aggaccaaca ttgcccacaa cgactaccag aaaaacaaca agggcaacca caaggggaac
     2941 aacaaagcca ataacaaggc taacgacaag gactctaaca acaacgcaaa caataatgct
     3001 aacaagaacg gcaacaatca caatgataac aacaacaaca accagaacaa ggacatcgac
     3061 atcaatagca actacgccat ccacaacaac aatgagaaag tgaagcggac caagaagaaa
     3121 atcaacgacg ctccctacga caagcaggat gaggaactgc ctagctacga cttcaacgat
     3181 ctgctccacg agaaaaagaa cggcgccagc gtgttcgact tcaatttcgt gctgttcctg
     3241 ttcatctgca tcaccttttt cggcgcgccg tcgacctcca tcacggccta taagagtgag
     3301 ggggagtcag cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag
     3361 ttgagatgga aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag
     3421 aaccaaaagg tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg
     3481 ctcccactca cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg
     3541 accctgactc tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg
     3601 actcagcccg acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg
     3661 agactgatct tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt
     3721 caagtgcagg cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc
     3781 aagatggact ccaagatcca ggttttatcc aaagggttga attccggcgg cggctccgga
     3841 ggcggtaccg gtggcggcga cctagcccca cagatgcttc gagaactcca ggagactaat
     3901 gcggcgctgc aagacgtgag agagctcttg cgacagcagg tcaaggagat caccttcctg
     3961 aagaatacgg tgatggaatg tgacgcctgc ggtctagaca gaaacctgcc tccactagca
     4021 cctctgggcc cacacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4081 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4141 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4201 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4261 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4321 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4381 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4441 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4501 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4561 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4621 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4681 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4741 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4801 gcctcactga ttaagcattg gggccctgac tacaaagacc atgacggtga ttataaagat
     4861 catgacatcg attacaagga tgacgatgac aagggccctc accaccacca tcaccactaa
     4921 taataaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     4981 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5041 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     5101 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     5161 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     5221 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     5281 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     5341 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     5401 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     5461 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     5521 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     5581 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     5641 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     5701 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     5761 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     5821 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     5881 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     5941 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     6001 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     6061 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     6121 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     6181 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     6241 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     6301 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     6361 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     6421 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     6481 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     6541 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     6601 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     6661 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     6721 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     6781 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     6841 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     6901 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     6961 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     7021 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     7081 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     7141 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     7201 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     7261 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7321 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7381 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7441 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7501 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7561 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7621 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     7681 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7741 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7801 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7861 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7921 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     7981 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8041 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8101 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8161 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8221 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8281 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8341 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8401 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8461 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8521 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8581 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8641 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8701 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8761 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8821 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8881 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8941 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9001 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9061 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9121 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9181 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9241 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9301 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9361 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9421 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     9481 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     9541 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     9601 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     9661 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
     9721 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     9781 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     9841 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     9901 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     9961 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
    10021 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
    10081 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
    10141 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
    10201 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
    10261 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
    10321 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
    10381 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
    10441 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
    10501 ct
//
