LOCUS       sequence-22463-e         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: LTRi Cre
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..92
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 agatctaagc ttggacaact acctacagag atttaaagct ctaaggtaaa tataaaattt
       61 actaggttgt ggaattgtga gcgctcacaa ttccacagtc gaccctaggg tgtggtgtgt
      121 taaactacgg atccgtctcc cattaggcct acaatggtga gacaagtagc caacagggaa
      181 gggttgcaaa tatcatttgg gcacacctat gataatattg atgaagcaca cagtattcag
      241 caagtaactg agaggtggga agctcaaagc caaagtccta atgtgcagtc aggtgaattt
      301 attgaaaaat ttgaggctcc tggtggtgca aatcaaagaa ctgctcctca gggatcctaa
      361 ttgtttgtgt attttatatt ccaaccaagc ttgcggccgc tcatgagcta aggaattgat
      421 cctctacagt cgacctgcag gaatgtgaaa tgcccaattt actgaccgta caccaaaatt
      481 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
      541 ttcgggatcg ccaggcgttt
//
