LOCUS       sequence-224638-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_EBFP2
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..41
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(91..105,106..111,112..128,129..135)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    143..159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             178..189
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             202..222
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    235..259
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(307..328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tntcntgacn ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga
       61 gcttaattaa caacaccatt tgtcgagaaa tcataaaaaa tttatttgct ttgtgagcgg
      121 ataacaatta taatagattc aattgtgagc ggataacaat ttcacacaga attcattaaa
      181 gaggagaaat taactatgaa acatcaccat caccatcacc atggtgctgc aggtacaagt
      241 ttgtacaaaa aagcaggctt catggtgagc aagggcgagg agctgttcac cggggtggtg
      301 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gaggggcgag
      361 ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
      421 ctgcccgtgc cctggcccac cctcgtgacc accctgagcc acggcgtggc agtgctttcg
      481 cccgcttacc ccgaaccaca tgaaagcagc ncgaactttc tttcaagtcc ccccatggcc
      541 ccaaagggct annntccagg aaannncacc canttttttt tcaagggann aaaggggacc
      601 ctnaaaaaaa cccccccccc caaaggnnaa aattnnngag ggggggaaac cccnnnaggg
      661 gaaacccccc attnnannnt ttaaaagggg gggtttgnaa atctctaaag gagagggaag
      721 gggggaaaaa nnnccgg
//
