LOCUS       sequence-224639-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_EBFP2
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..45
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caggctcngc nnntnnnctt accactttgt acaagaaagc tgggtcttac ttgtacagct
       61 cgtccatgcc gagagtgatc ccggcggcgg tgcggaactc cagcaggacc atgtgatcgc
      121 gcttctcgtt ggggtctttg ctcagcacgg actgggtgct caggtagtgg ctgtcgggca
      181 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      241 tgccgtcctc cacgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttga
      301 cggccatgat atagatgttg tggctgttga agttgtactc cagcttgtgc cccaggatgt
      361 tgccgtcctc cttgaagtcg acgcccttca gctcgatgcg gttcactagg gtgtcgccct
      421 cgaacttcac ctcggcgcgg gtcttgtagg tgccgtcgtc cttgaagaag atggtgcgct
      481 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      541 ggtagcgggc gaagcactgc acgccnnggc tcaggggggg ncannnaggg ngggcccagg
      601 gcacggggca gcttgccngg gggggcnnaa nnaacttnnn ggggcaacct tnnccnttgg
      661 ggggnntngg nnnnngnccc
//
