LOCUS       sequence-224644-        1174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_EBFP2-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..1174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(72..74,75..77,78..788)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(117..138)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttaactatga acatcaccat caccatcacc atggtgctgc aggtacaagt ttgtacaaaa
       61 aagcaggctt catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gaggggcgag ggcgagggcg
      181 atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      241 cctggcccac cctcgtgacc accctgagcc acggcgtgca gtgcttcgcc cgctaccccg
      301 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      361 gcaccatctt cttcaaggac gacggcacct acaagacccg cgccgaggtg aagttcgagg
      421 gcgacaccct agtgaaccgc atcgagctga agggcgtcga cttcaaggag gacggcaaca
      481 tcctggggca caagctggag tacaacttca acagccacaa catctatatc atggccgtca
      541 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacgtggag gacggcagcg
      601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      661 ccgacagcca ctacctgagc acccagtccg tgctgagcaa agaccccaac gagaagcgcg
      721 atcacatggt cctgctggag ttccgcaccg ccgccgggat cactctcggc atggacgagc
      781 tgtacaagac cggtgccgga ggtgggggcc taggaatggt gagcaagggc gaggagaata
      841 acatggccat catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
      901 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc tttcagaccg
      961 ttaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ttgtcccctc
     1021 agttcaccta cggctccaag gcctacgtga agcaccccgc cgaantcccc gaatacctna
     1081 agctgtcctt nnccgagggc ttcaannngg aaccnnngat gaanttcaag nanngnggnc
     1141 nngnngaccn nnannnngga nnnnnnctgn ngga
//
