LOCUS       sequence-224645-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_EBFP2-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(54..764)
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(332..351)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     gap             958..968
                     /estimated_length=11
ORIGIN
        1 gnncagctcn gcnnntnnct taccactttg tacaagaaag ctgggtcaag cttttacttg
       61 tacagctcgt ccatgcctcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
      121 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgtcgacgat gtaggcgccg
      181 ggcaactgca cgggcttctt ggccttgtag gtggtcttga cctcggaggt gtagtggccg
      241 ccgtccttca gcttcagcct gagcttgtcc tcgcccttca gggcgccgtc ctcggggtac
      301 atccgctcgg aggaggcctc catgcccatg gtcttcttct gcattacggg gccgtcggag
      361 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
      421 tcctgagtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      481 ccctcgggga aggacagctt gaggtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      541 ttggagccgt aggtgaactg aggggacaag atgtcccagg cgaagggcag ggggccaccc
      601 ttggtcacct tcagcttaac ggtctgaaag ccctcgtagg ggcggccctc gccctcgccc
      661 tcgatctcga actcgtggcc gttcacggag ccctccatgc gcaccttgaa gcgcatgaac
      721 tccttgatga tggccatgtt attctcctcg cccttgctca ccattcctag gcccccacct
      781 ccggcaccgg tcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtgcggaac
      841 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcancca cggactgggt
      901 gctcaggtag tggctgtcgg gcagcaccnc ggggccgtcc cccanggggg tgttctgnnn
      961 nnnnnnnncc gccnncnnnc cccnnccccc ccccc
//
