LOCUS       sequence-224646-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_ECFP
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..51
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(98..119)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gnnnnnnnnc tnagctnntt aagcttacca ctttgtacaa gaaagctggg tcttacttgt
       61 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      121 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
      181 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      241 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct
      301 gcttgtcggc ggtgatatag acgttgtggc tgatgtagtt gtactccagc ttgtgcccca
      361 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
      421 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgcccttg aagaagatgg
      481 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      541 ggtcggggna gcggctgaag cactgcacgc cccangtcag gntggtcacn nnggnnngnn
      601 acggcacggg naccccgncg gngnncnnnn nnnnncnnnc acgnnnnnc
//
