LOCUS       sequence-224647-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_mTFP1
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..39
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(89..103,104..109,110..126,127..133)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    109..125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    141..157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             176..187
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             200..220
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    233..257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             260..970
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDXLYK"
     protein_bind    complement(972..996)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tnntgncntt aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgagc
       61 ttaattaaca acaccatttg tcgagaaatc ataaaaaatt tatttgcttt gtgagcggat
      121 aacaattata atagattcaa ttgtgagcgg ataacaattt cacacagaat tcattaaaga
      181 ggagaaatta actatgaaac atcaccatca ccatcaccat ggtgctgcag gtacaagttt
      241 gtacaaaaaa gcaggcttca tggtgagcaa gggcgaggag accacaatgg gcgtaatcaa
      301 gcccgacatg aagatcaagc tgaagatgga gggcaacgtg aatggccacg ccttcgtgat
      361 cgagggcgag ggcgagggca agccctacga cggcaccaac accatcaacc tggaggtgaa
      421 ggagggagcc cccctgccct tctcctacga cattctgacc accgcgttcg cctacggcaa
      481 cagggccttc accaagtacc ccgacgacat ccccaactac ttcaagcagt ccttccccga
      541 gggctactct tgggagcgca ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc
      601 cgacatctcc atggaggagg actccttcat ctacgagata cacctcaagg gcgagaactt
      661 cccccccaac ggccccgtga tgcagaagaa gaccaccggc tgggacgcct ccaccgagag
      721 gatgtacgtg cgcgacggcg tgctgaaggg cgacgtcaag cacaagctgc tgctggaggg
      781 cggcggccac caccgcgttg acttcaagac catctacagg gccaagaagg cggtgaagct
      841 gcccgactat cactttgtgg accaccgcat cgagatcctg aaccacgaca aggactacaa
      901 caaggtgacc gtttacgaga gcgccgtggc ccgcaactcc accgacggca tggacganct
      961 gtacaagtaa gacccagctt tcttgtacaa agtggtaagc ttaattanct gaacttggac
     1021 tcctgttgaa aaatccanta atgacctcan aanctccnnc tggaattntt caaancgctc
     1081 ggttgccccn gggnnntntt tattggtgaa aatccaatta nnnccccntt ttaatggaat
     1141 ggttaaantg gaangnt
//
