LOCUS       sequence-224648-        1141 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_mTFP1-mVenus
FEATURES             Location/Qualifiers
     source          1..1141
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(88..102,103..108,109..125,126..132)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    108..124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             175..186
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             199..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    232..256
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             259..966
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(1039..1060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tnntgacntt aacctatann ataggcgtat cacgaggccc tttcgtcttc acctcgagct
       61 taattaacaa caccatttgt cgagaaatca taaaaaattt atttgctttg tgagcggata
      121 acaattataa tagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag
      181 gagaaattaa ctatgaaaca tcaccatcac catcaccatg gtgctgcagg tacaagtttg
      241 tacaaaaaag caggcttcat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag
      301 cccgacatga agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc
      361 gagggcgagg gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag
      421 gagggagccc ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac
      481 agggccttca ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag
      541 ggctactctt gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc
      601 gacatctcca tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc
      661 ccccccaacg gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg
      721 atgtacgtgc gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc
      781 ggcggccacc accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg
      841 cccgactatc actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac
      901 aaggtgaccg tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg
      961 tacaagaccg gtgccggagg tgggggccta ggaatggtga gcaagggcga ggagctgttc
     1021 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcnn naagttcagc
     1081 gtgtccggcg agggcgaggg cgatgcccnt acggcaagct gaccctgaag ctgatctgnn
     1141 c
//
