LOCUS       sequence-224649-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_mTFP1-mVenus
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(88..109)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(244..756,757..759,760..762))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     437..456
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     696..717
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagctcngct attnngctta ccactttgta caagaaagct gggtcaagct tttactcgtc
       61 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      121 ctcgttgggg tctttgctca gcttggactg gtagctcagg tagtggttgt cgggcagcag
      181 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgccgcc
      241 gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc
      301 ggtgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
      361 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      421 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      481 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      541 gcgggcgaag cactgcaggc cgtagcccag ggtggtcacg agggtgggcc agggcacggg
      601 cagcttgccg gtggtgcaga tcagcttcag ggtcagcttg ccgtaggtgg catcgccctc
      661 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      721 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc attcctaggc ccccacctcc
      781 ggcaccggtc ttgtacagct cgtccatgcc gtcggtggag ttgcgggcca cggcgctctc
      841 gtaaacggtc accttgttgt agtccttgtc gtggttcagg atctcgatgc ggtggtccac
      901 aaagtgatag tcgggcagct tcaccgcctt cttggccctg tagatggtct tgaagtcaac
      961 gcggtggtgg ccgccgccct ccagcagcag cttgtgcttg acgtcgccct tcagcacgcc
     1021 gtcgcgcacg tacatcctcn cnggtggagg cgtcccagcc ggtggtcttc ttctgcatcc
     1081 nggggccgtt gggggngnaa gtttt
//
