LOCUS       sequence-22465-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: LTRi Cre
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..74)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(56..74)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(102..121)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    565..584
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatatcaa ttcgccctat
       61 agtgagtcgt attacaattc tttgccaaaa tgatgagaca gcacaataac cagcacgttg
      121 cccaggagct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggg gcccgttctt
      181 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      241 cttccttagc tcctgagcgg ccgcaagctt ggttggaatc taaaatacac aaacaattag
      301 gatccctgag gagcagttct ttgatttgca ccaccaggag cctcaaattt ttcaataaat
      361 tcacctgact gcacattagg actttggctt tgagcttccc acctctcagt tacttgctga
      421 atactgtctg cttcatcaat attatcatag gtgtgcccaa atgatatttg caacccttcc
      481 ctgttggcta cttgtctcac cattgtaggc ctaatgggag acggatccgt agtttaacac
      541 attatacact agggtcgact gtggaattgt gagcgctcac aattccacaa cctagggtcg
      601 actgtggaa
//
