LOCUS       sequence-224650-         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_LSSmOrange
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(326..345)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cnnnctcngc taattaagct taccactttg tacaagaaag ctgggtctta cttgtacagc
       61 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
      121 tagtcctcgt tgtgggaggt gatgtccaac ttgatgtcga cgatgtaggc gccgggcaac
      181 tgcacgggct tcttggcctt gtaggtggtc ttgacctcgg aggtgtagtg gccgccgtcc
      241 ttcagcttca gcctgagctt gtcctcgccc ttcagggcgc cgtcctcggg gtacatccgc
      301 tcggaggagg cctccatgcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
      361 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctga
      421 gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
      481 gggaaggaca gcttgaggta gtcggggatg tcggcggggt gcttcacgta ggccttggag
      541 ccgtaggtga actgagggga caagatgtcc caggcgaagg gcagggggcc acccttggtc
      601 accttcagct taacggtctg aaagccctcg taggggcggc cctcgccctc gccctcgatn
      661 tcnaactcgt ggccgttcac ggagccctcc atgnncncct tgaagngcan gaannccttg
      721 aagaaggcnn nnt
//
