LOCUS       sequence-22466-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: LTRi with neomycin
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..80)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(62..80)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(108..127)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    571..590
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(708..759)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(737..757)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 taagcagctc taatgcgctg ttatcacttt acttttatct aatctagaca tatcaattcg
       61 ccctatagtg agtcgtatta caattctttg ccaaaatgat gagacagcac aataaccagc
      121 acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggggccc
      181 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      241 ttttagcttc cttagctcct gagcggccgc aagcttggtt ggaatctaaa atacacaaac
      301 aattaggatc cctgaggagc agttctttga tttgcaccac caggagcctc aaatttttca
      361 ataaattcac ctgactgcac attaggactt tggctttgag cttcccacct ctcagttact
      421 tgctgaatac tgtctgcttc atcaatatta tcataggtgt gcccaaatga tatttgcaac
      481 ccttccctgt tggctacttg tctcaccatt gtaggcctaa tgggagacgg atccgtagtt
      541 taacacatta tacactaggg tcgactgtgg aattgtgagc gctcacaatt ccacaaccta
      601 gtaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc caagcttaga
      661 tcctgcaggt cgactctaga ggatccgatc tgacggttca ctaaaccagc tctgcttata
      721 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggc
//
