LOCUS       sequence-224719-       12478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the E2F1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-E2F1
FEATURES             Location/Qualifiers
     source          1..12478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2144..3322
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2375..3313
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3270..3290
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3370..3487
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3519..3543
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4863..4887)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4911..5478
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4929..5480
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5073..5090)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5300..5319
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5483..5485,5486..5488,5489..6199)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5483..5485,5486..5488,5489..6199)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5528..5549)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5789..5808)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6136..6157
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6278..6294)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6279..6295)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6308..6896
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6361..6381)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6779..6790)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7040..7073)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7093..7273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7298..7316)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7299..7316)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7606..7625)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7839..7858)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7958..7980
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8018..8036)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8104..8208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8209..8277,8278..9069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8427..8446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9240..9828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9729..9748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9982..9999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10069..10089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10074..10403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10254..10389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11537..11602
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11732..11752
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12177..12311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12214..12233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12268..12287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgt cgacgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
     2101 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     2161 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2221 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2281 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2341 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2401 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2461 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2521 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2581 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2641 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2701 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2761 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2821 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2881 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2941 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3001 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3061 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3121 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3181 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3241 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3301 tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaat ctcgacggta
     3361 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     3421 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     3481 aaattttatc gatcacgaga ctagcctcga ggtttatcac aagtttgtac aaaaaagcag
     3541 gcttcaccat ggccttggcc ggggcccctg cgggcggccc atgcgcgccg gcgctggagg
     3601 ccctgctcgg ggccggcgcg ctgcggctgc tcgactcctc gcagatcgtc atcatctccg
     3661 ccgcgcagga cgccagcgcc ccgccggctc ccaccggccc cgcggcgccc gccgccggcc
     3721 cctgcgaccc tgacctgctg ctcttcgcca caccgcaggc gccccggccc acacccagtg
     3781 cgccgcggcc cgcgctcggc cgcccgccgg tgaagcggag gctggacctg gaaactgacc
     3841 atcagtacct ggccgagagc agtgggccag ctcggggcag aggccgccat ccaggaaaag
     3901 gtgtgaaatc cccgggggag aagtcacgct atgagacctc actgaatctg accaccaagc
     3961 gcttcctgga gctgctgagc cactcggctg acggtgtcgt cgacctgaac tgggctgccg
     4021 aggtgctgaa ggtgcagaag cggcgcatct atgacatcac caacgtcctt gagggcatcc
     4081 agctcattgc caagaagtcc aagaaccaca tccagtggct gggcagccac accacagtgg
     4141 gcgtcggcgg acggcttgag gggttgaccc aggacctccg acagctgcag gagagcgagc
     4201 agcagctgga ccacctgatg aatatctgta ctacgcagct gcgcctgctc tccgaggaca
     4261 ctgacagcca gcgcctggcc tacgtgacgt gtcaggacct tcgtagcatt gcagaccctg
     4321 cagagcagat ggttatggtg atcaaagccc ctcctgagac ccagctccaa gccgtggact
     4381 cttcggagaa ctttcagatc tcccttaaga gcaaacaagg cccgatcgat gttttcctgt
     4441 gccctgagga gaccgtaggt gggatcagcc ctgggaagac cccatcccag gaggtcactt
     4501 ctaaggagga gaacagggcc actgactctg ccaccatagt gtcaccacca ccatcatctc
     4561 ccccctcatc cctcaccaca gatcccagcc agtctctact cagcctggag caagaaccgc
     4621 tgttgtcccg gatgggcagc ctgcgggctc ccgtggacga ggaccgcctg tccccgctgg
     4681 tggcggccga ctcgctcctg gagcatgtgc gggaggactt ctccggcctc ctccctgagg
     4741 agttcatcag cctttcccca ccccacgagg ccctcgacta ccacttcggc ctcgaggagg
     4801 gcgagggcat cagagacctc ttcgactgtg actttgggga cctcaccccc ctggatttct
     4861 gaacccagct ttcttgtaca aagtggtgat aaactacggg ctgcaggaat tccgcccccc
     4921 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     4981 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5041 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5101 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5161 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5221 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5281 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     5341 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     5401 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     5461 tgaaaaacac gatgataata ccatggtgag caagggcgag gagctgttca ccggggtggt
     5521 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5581 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5641 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5701 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5761 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5821 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5881 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5941 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6001 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6061 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6121 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6181 catggacgag ctgtacaagt ccggactcag atctcgacta gctagtagct agctagctag
     6241 tcgagctcaa gcttcgaatt cgatatcaag cttatcgcga taccgtcgac ctcgagggaa
     6301 ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6361 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6421 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6481 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6541 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6601 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6661 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6721 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6781 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6841 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     6901 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6961 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatg
     7021 ggatcaattc accatgggaa taacttcgta tagcatacat tatacgaagt tatgctgctt
     7081 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7141 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7201 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7261 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
     7321 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
     7381 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
     7441 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
     7501 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
     7561 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     7621 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     7681 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     7741 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     7801 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
     7861 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7921 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7981 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     8041 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8101 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8161 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8221 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8281 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8341 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8401 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8461 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8521 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8581 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8641 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8701 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8761 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8821 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8881 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8941 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9001 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9061 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9121 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9181 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9241 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9301 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9361 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9421 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9481 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9541 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9601 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9661 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9721 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9781 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9841 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9901 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9961 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10021 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc
    10081 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    10141 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    10201 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    10261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    10321 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10381 ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg
    10441 agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt
    10501 gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg
    10561 aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc
    10621 gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct
    10681 gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg
    10741 cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca
    10801 accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta
    10861 ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag
    10921 cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg
    10981 ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc
    11041 cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta
    11101 acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag
    11161 gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta
    11221 gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc
    11281 atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca
    11341 cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat
    11401 gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg
    11461 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
    11521 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
    11581 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
    11641 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
    11701 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
    11761 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
    11821 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
    11881 tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc ataacatact
    11941 gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt
    12001 gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc
    12061 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
    12121 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    12181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    12241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12301 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    12361 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    12421 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
