LOCUS       sequence-224722-        6012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red fluorescent voltage indicator for neuronal expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Kannan M, Vasan G, Huang C, Haziza S, Li JZ, Inan H, Schnitzer MJ, 
            Pieribone VA
  TITLE     Fast, in vivo voltage imaging using a red fluorescent indicator.
  JOURNAL   Nat Methods. 2018 Dec;15(12):1108-1116. doi: 
            10.1038/s41592-018-0188-7. Epub 2018 Nov 12.
  PUBMED    30420685
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0188-7"; journalName: "Nat Methods"; date: 
            "2018-12"; volume: "15"; issue: "12"; pages: "1108-1116"
COMMENT     Sequence Label: pAAV-Syn-VARNAM
FEATURES             Location/Qualifiers
     source          1..6012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   44..173
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        218..665
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      685..694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2182..2770
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2235..2255)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2653..2664)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2802..3278
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3001..3020)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3318..3458
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3318..3447
                     /label=AAV2 ITR
     rep_origin      3533..3988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3620..3639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3830..3851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4005..4024)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4124..4146
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4184..4202)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4270..4374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4375..4443,4444..5235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4593..4612)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5406..5994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5895..5914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc
       61 gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt
      121 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg
      181 cacgcgtgtg tctagactgc agagggccct gcgtatgagt gcaagtgggt tttaggacca
      241 ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg gacaagcacc
      301 caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga aacaggatgc
      361 ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg cccccgcctg
      421 gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc gccggtcccc
      481 cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc ccagccggac
      541 cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc tgcggcgccg
      601 gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag
      661 cgcagtcgag aaggtaccgg atccgccacc atggctgacg tggaaaccga gaccggcatg
      721 attgcacagt ggattgtctt tgctattatg gctgctgctg ctattgcttt tggagtggct
      781 gtgcactttc ggccttcaga gctgaagagc gcatactata tcaacattgc catctgcact
      841 atcgccgcta ccgcttacta tgcaatggcc gtgaactacc aggacctgac aatgaatggt
      901 gaaaggcagg tggtctacgc aagatatatt agctgggtgc tgaccacacc actgctcctg
      961 ctcgatctca tcgtcatgac caagatgggc ggagtgatga tttcttgggt catcggcgca
     1021 gacattttca tgatcgtgtt tggtattctg ggcgccttcg aggatgaaca caagttcaaa
     1081 tgggtgtact ttatcgctgg atgtgtgatg caggcagtcc tgacatacgg gatgtataac
     1141 gccacttgga aagacgatct gaagaaaagc cccgagtacc atagctccta tgtcagtctg
     1201 ctcgtcttcc tgtcaatcct ctgggtgttt tatcctgtcg tgtgggcttt cgggtctggt
     1261 agtggcgtgc tgtccgtcga caatgaggcc attctcatgg gaatcctgga tgtgctcgct
     1321 aagccactgt ttggaatggg gtgcctcatt gcccatgaga ctatcttcaa gtggaggtcc
     1381 ggactgatta aggaaaatat gcggatgaag gtcgtgatgg aagggtctgt caatgggcac
     1441 cagttcaagt gcaccggaga gggagagggc aggccatacg agggcgtgca gacaatgagg
     1501 atcaaagtga tcgagggagg accactgcct ttcgcctttg acatcctggc caccagcttc
     1561 atgtacggca gcaggacctt catcaagtat ccagccgaca tccccgattt ctttaagcag
     1621 agcttccccg agggctttac ctgggagagg gtgacaagat acgaggatgg cggcgtggtg
     1681 accgtgacac aggacacctc tctggaggat ggcgagctgg tgtataacgt gaaggtgagg
     1741 ggcgtgaact tccctagcaa tggcccagtg atgcagaaga agaccaaggg ctgggagccc
     1801 aatacagaga tgatgtaccc tgcagacgga ggcctgaggg gatataccga catcgccctg
     1861 aaggtggatg gaggaggaca cctgcactgt aactttgtga ccacataccg ctccaagaag
     1921 acagtgggca atatcaagat gccaggagtg cacgccgtgg accacaggct ggagcgcatc
     1981 gaggagtctg ataacgagac atatgtggtg cagagagagg tggccgtggc caagtactct
     2041 aatctgggcg gcgggatgga cgagctgtat aagaagagca ggatcaccag cgagggcgag
     2101 tacatccccc tggaccagat cgacatcaac gtgttctgct acgagaacga ggtgtaagaa
     2161 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     2221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2581 tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc
     2641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2701 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2761 gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc
     2821 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     2881 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     2941 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     3001 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     3061 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     3121 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     3181 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     3241 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt
     3301 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3361 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3421 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     3481 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     3541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3601 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3721 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     3781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3841 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     3901 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3961 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     4021 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4081 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4141 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     4201 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4321 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4381 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4441 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4501 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4561 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4621 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4681 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4741 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4801 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4861 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4921 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4981 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5041 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5101 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5161 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5221 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5281 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5341 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5521 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5761 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6001 agcaacgcgg cc
//
