LOCUS       sequence-224739-        8006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of truncated Rag1 including the RING, E3 ubiquitin ligase
            (265-1008) and C-terminal region (384-1040).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Grundy GJ, Yang W, Gellert M
  TITLE     Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome
            by an epigenetic signal in V(D)J recombination.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22487-92. doi: 
            10.1073/pnas.1014958107. Epub 2010 Dec 13.
  PUBMED    21149691
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1014958107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-12-28- 28"; volume: "107"; issue: "52"; pages: 
            "22487-92"
COMMENT     Sequence Label: UR1Ct (265-1040)
FEATURES             Location/Qualifiers
     source          1..8006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1214..1223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1226..1249
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1256..2353
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2210..2219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2327..2350
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2402..2425
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(4865..4884)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4930..4985
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4984..5003)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5346..5362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5346..5362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5359..5381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5370..5386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5394..5400,5401..5418,5419..5424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5439..5460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5589..5606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5760..6348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5840..5859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6519..7310,7311..7379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7142..7161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7380..7484)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7515..7894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgcggccgtc
     1201 tcaggccgaa ttcgccacca tggtgcatca ccatcaccat caccatcatc gatccatgat
     1261 cgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga
     1321 agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa
     1381 actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg
     1441 ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga
     1501 caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa
     1561 gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct
     1621 gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa
     1681 aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc
     1741 tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg
     1801 cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa
     1861 acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac
     1921 agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta
     1981 tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct
     2041 gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa
     2101 ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt
     2161 agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga
     2221 aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc
     2281 cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa
     2341 agacgcgcag actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg
     2401 gctcgaagtc ctattccagg gaccggtgaa ctgcagtaag attcatctca gtaccaagct
     2461 tcttgccgtg gacttcccag cacactttgt gaaatccatc tcctgccaga tatgcgaaca
     2521 cattctggct gatcccgtgg agaccagctg caagcatcta ttctgtagga tctgcattct
     2581 cagatgtctc aaagtcatgg gcagctattg tccctcttgc cgatatccgt gcttccctac
     2641 tgacctggag agcccagtga agtcctttct gaacatcttg aattctctca tggtcaagtg
     2701 tcccgcgcaa gattgcaatg aggaagtgag tctggaaaaa tataaccacc atgtgtcaag
     2761 ccacaaagaa tctaaagaga ctttggtgca tatcaataaa gggggacggc ctcgccagca
     2821 tctcctgtca ctgacgagaa gggcgcagaa acatcggctg agggagctca agattcaagt
     2881 caaagaattt gctgacaaag aagaaggtgg agatgtgaag gctgtctgct tgacattgtt
     2941 tctcctggca ctgagggcga ggaatgagca caggcaagct gatgaattag aggccatcat
     3001 gcaaggcagg ggctccgggc ttcaaccagc tgtttgcttg gccatccgtg tcaatacctt
     3061 cctcagctgt agccaatacc ataagatgta caggactgtg aaagctatca ctgggaggca
     3121 gatttttcaa cctttgcatg ctcttcggaa tgccgagaaa gtccttctgc caggctacca
     3181 tccctttgag tggcagcccc cactgaagaa tgtgtcctcc agaactgatg ttggaattat
     3241 tgatgggctg tctggacttg cctcctctgt ggatgagtac ccagtagata ccattgcgaa
     3301 gaggttccgc tacgactctg ctttggtgtc tgctttgatg gacatggaag aagacatctt
     3361 ggaaggcatg agatcccaag atcttgatga ctacctgaat ggtcccttca cagtggtggt
     3421 aaaggagtct tgcgatggaa tgggggatgt gagtgagaag cacgggagtg ggcccgcagt
     3481 tccagaaaag gccgttcgtt tctctttcac agtcatgaga attacgatag agcatggttc
     3541 acagaacgtg aaggtgtttg aggaacccaa gcccaattct gaactgtgtt gcaagccgtt
     3601 gtgtcttatg ctggcagatg agtctgacca tgagaccctt actgctattc taagccccct
     3661 cattgccgag agggaggcca tgaagagcag tgaattaacg ctggagatgg gaggcatccc
     3721 caggactttt aaattcatct tcaggggcac tggatacgat gaaaaacttg tccgggaagt
     3781 agaaggcttg gaagcttctg gctcagtcta catctgtaca ctctgtgaca ccacccgttt
     3841 ggaagcctct cagaatcttg tcttccactc cataaccaga agccacgccg agaacctgca
     3901 gcgctatgag gtctggcggt ccaatccgta tcatgagtcc gtggaagagc tccgggaccg
     3961 ggtgaaaggg gtctctgcca aacctttcat cgagacagtc ccttccatag atgcgcttca
     4021 ctgtgacatt ggcaatgcag ctgaattcta taagattttc cagctggaga taggggaagt
     4081 gtataaacat cccaatgcct ctaaagagga aaggaagaga tggcaggcca cgctggacaa
     4141 acatctccgg aaaaggatga acttaaaacc aatcatgagg atgaatggca actttgcccg
     4201 gaagcttatg acccaagaga ctgtagacgc agtttgtgag ttaattcctt ctgaggagag
     4261 gcatgaagct ctcagggagc tcatggacct ttacctgaag atgaaacccg tgtggcgctc
     4321 ttcatgtccc gctaaagagt gtccagagtc cctctgtcag tacagtttca actcacagcg
     4381 tttcgcggaa ctcctctcca ccaagttcaa atatagatac gagggcaaaa tcaccaatta
     4441 ctttcacaaa accttggcac atgtccctga aattattgaa agggatggct ctatcggggc
     4501 ctgggcaagt gagggaaatg aatcgggtaa caagctgttt agacggtttc ggaaaatgaa
     4561 tgccaggcag tccaagtgct atgagatgga agatgtcctg aaacatcact ggctgtatac
     4621 ttcaaaatac ctccagaagt ttatgaatgc tcataacgcg ttaaaaagct ctgggtttac
     4681 catgaactca aaggagacct taggggaccc tttgggcatt gaggactctc tggaaagcca
     4741 agattcaatg gagttttaat aactcgagac tagtatcgcg ataattcact cctcaggtgc
     4801 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     4861 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     4921 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     4981 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     5041 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     5101 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     5161 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     5221 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     5281 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
     5341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatcgat
     5521 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7381 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7441 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7501 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     7561 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     7621 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     7681 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     7741 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     7801 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     7861 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     7921 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     7981 attttgtgca gcgatggggg cggggg
//
