LOCUS       sequence-224745-       11397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses IncB tagged with 7 copies of GFP11 under a tetracycline 
            inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Wang X, Hybiske K, Stephens RS
  TITLE     Direct visualization of the expression and localization of 
            chlamydial effector proteins within infected host cells.
  JOURNAL   Pathog Dis. 2018 Mar 1;76(2). pii: 4830102. doi: 
            10.1093/femspd/fty011.
  PUBMED    29390129
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pathog 
            Dis."; date: "2018-03-1"; volume: "76(2). pii: 4830102. doi"; pages:
            " 10.1093/femspd/fty011"
COMMENT     Sequence Label: pTL2-IncB-GFP11x7
FEATURES             Location/Qualifiers
     source          1..11397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     31..52
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        216..320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(321..389,390..1181)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAALCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRST
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(539..558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             1188..1814
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1264..1283)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     rep_origin      1967..2555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2456..2475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2635..2653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2660..2678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3092..3517
                     /codon_start=1
                     /product="seven tandem copies of the 11th beta-strand of 
                     superfolder GFP"
                     /label=GFP11x7
                     /note="each GFP11 associates with GFP(1-10), consisting of 
                     the first 10 beta-strands, to reconstitute fluorescent GFP 
                     (Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDH
                     MVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSG
                     GRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGIT"
     CDS             3521..3544
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3722..3741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
       61 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      121 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      181 ttacaatttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      241 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      301 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      361 ttgcggcact ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      421 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      481 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      541 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      601 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      661 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      721 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      781 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      841 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      901 gcgaactact tactctagct tcccggcaac aattgataga ctggatggag gcggataaag
      961 ttgcaggacc acttctgcgc tcgacccttc cggctggctg gtttattgct gataaatctg
     1021 gagccggtga gcgtggctct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1081 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1141 agatcgctga gataggtgcc tcactgatta agcattggta ggaattaatg atgtctcgtt
     1201 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     1261 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     1321 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     1381 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     1441 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     1501 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     1561 gtttttcact agagaatgca ttatatgcac tcagcgcagt ggggcatttt actttaggtt
     1621 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     1681 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     1741 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     1801 aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg gtaacttcac tagtttaaaa
     1861 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1921 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1981 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2041 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2101 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2161 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2221 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2281 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2341 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2401 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2461 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2521 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2581 ggttcctggc cttttgctgg ccttttgctc acatgacccg acaccatcga atggtcccta
     2641 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat cagcaacttt
     2701 aagaaggaga tcggccgaca cagagggaaa tatggttcat tctgtataca attcattggc
     2761 tccagaaggt tttagccaag tctctattca acccagtcag attccaacca gcaaaaaagt
     2821 aatgattgcg ataatgactc tttttgcact cacagccatt gcagcaatag tcctttccat
     2881 cgttacagtt tgtggagggt ttccttttct tcttgctgca cttaacaccg taactattgg
     2941 tgcatgcgta tccttgccga tatttacttg catagctaca acgttattac ttctttgtct
     3001 ccgtaatatc gaactcctag ccagaccgca agtattgacc ctctccactc aattcagccc
     3061 aacaaaacct caagttgtcg acggttcggg acgtgaccac atggtccttc atgagtatgt
     3121 aaatgctgct gggattacag gtggctctgg aggtagagat catatggttc tccacgaata
     3181 cgttaacgcc gcaggcatca ctggcggtag tggaggacgc gaccatatgg tactacatga
     3241 atatgtcaat gcagccggaa taaccggagg gtccggaggc cgggatcaca tggtgctgca
     3301 tgagtatgtg aacgcggcgg gtataactgg tgggtcgggc ggacgagacc atatggtgct
     3361 tcacgaatac gtaaacgcag ctggcattac tggcggatca ggtggcaggg atcacatggt
     3421 actccatgag tacgtgaacg ctgctggaat cacaggcggt agcggcggtc gggaccatat
     3481 ggtcctgcac gaatatgtca atgctgccgg tatcaccatg gactacaagg atgacgacga
     3541 taaggctagc taatgacctg tgaagtgaaa aatggcgcac attgtgcgac attttttttg
     3601 tctgccgttt accgctactg cgtcacggat ctccacgcgc cctgtagcgg cgcattaagc
     3661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3781 ctaaagggcc caagaggtaa gtcctctagt acaaacaccc ccaatattgt gatataatta
     3841 aaattatatt catattctgt tgccagaaaa aacactttta ggctatatta gagccatctt
     3901 ctttgaagcg ttgtcttctc gagaagattt atcgtacgca aatatcatct ttgcggttgc
     3961 gtgtcctgtg accttcatta tgtcggagtc tgagcaccct aggcgtttgt actccgtcac
     4021 agcggttgct cgaagcacgt gcggggttat cttaaaaggg attgcagctt gtagtcctgc
     4081 ttgagagaac gtgcgggcga tttgccttaa ccccaccatt tttccggagc gagttacgaa
     4141 gacaaaacct cttcgttgac cgatgtactc ttgtagaaag tgcataaact tctgaggata
     4201 agttataata atcctctttt ctgtctgacg gttcttaagc tgggagaaag aaatggtagc
     4261 ttgttggaaa caaatctgac taatctccaa gcttaagact tcagaggagc gtttacctcc
     4321 ttggagcatt gtctgggcga tcaaccaatc ccgggcattg atttttttta gctcttttag
     4381 gaaggacgct gtttgcaaac tgttcatcgc atctgttttt actatttccc tggttttaaa
     4441 aaatgttcga ctattttctt gtttagaagg ttgcgctata gcgactattc cttgagtcat
     4501 cctgtttagg aatcttgtta aggaaatata gcttgctgct cgaacttgtt tagtaccttc
     4561 ggtccaagaa gtcttggcag aggaaacttt tttaatcgca tctagaatta gattatgatt
     4621 taaaagggaa aactcttgca gattcatatc caaggacaat agaccaatct tttctaaaga
     4681 caaaaaagat cctcgatatg atctacaagt atgtttgttg agtgatgcgg tccaatgcat
     4741 aataacttcg aataaggaga agcttttcat gcgtttccaa taggattctt ggcgaatttt
     4801 taaaacttcc tgataagact tttcgctata ttctaacgac atttcttgct gcaaagataa
     4861 aatcccttta cccatgaaat ccctcgtgat ataacctatc cgtaaaatgt cctgattagt
     4921 gaaataatca ggttgttaac aggatagcac gctcggtatt tttttatata aacatgaaaa
     4981 ctcgttccga aatagaaaat cgcatgcaag atatcgagta tgcgttgtta ggtaaagctc
     5041 tgatatttga agactctact gagtatattc tgaggcagct tgctaattat gagtttaagt
     5101 gttctcatca taaaaacata ttcatagtat ttaaatactt aaaagacaat ggattaccta
     5161 taactgtaga ctcggcttgg gaagagcttt tgcggcgtcg tatcaaagat atggacaaat
     5221 cgtatctcgg gttaatgttg catgatgctt tatcaaatga caagcttaga tccgtttctc
     5281 atacggtttt cctcgatgat ttgagcgtgt gtagcgctga agaaaatttg agtaatttca
     5341 ttttccgctc gtttaatgag tacaatgaaa atccattgcg tagatctccg tttctattgc
     5401 ttgagcgtat aaagggaagg cttgacagtg ctatagcaaa gactttttct attcgcagcg
     5461 ctagaggccg gtctatttat gatatattct cacagtcaga aattggagtg ctggctcgta
     5521 taaaaaaaag acgagcaacg ttctctgaga atcaaaattc tttctttgat gccttcccaa
     5581 caggatacaa ggatattgat gataaaggag ttatcttagc taaaggtaat ttcgtgatta
     5641 tagcagctag gccatctata gggaaaactg ctttagctat agacatggcg ataaatcttg
     5701 cggttactca acagcgtaga gttggtttcc tatctctaga aatgagcgca ggtcaaattg
     5761 ttgagcggat tattgctaat ttaacaggaa tatctggtga aaaattacaa agaggggatc
     5821 tctctaaaga agaattattc cgagtagaag aagctggaga aacagttaga gaatcacatt
     5881 tttatatctg cagtgatagt cagtataagc ttaatttaat cgcgaatcag atccggttgc
     5941 tgagaaagga agatcgagta gacgtaatat ttatcgatta cttgcagttg atcaactcat
     6001 cggttggaga aaatcgtcaa aatgaaatag cagatatatc tagaacctta agaggtttag
     6061 cctcagagct aaacattcct atagtttgtt tatcccaact atctagaaaa gttgaggata
     6121 gagcaaataa agttcccatg ctttcagatt tgcgagacag cggtcaaata gagcaagacg
     6181 cagatgtgat tttgtttatc aataggaagg aatcgtcttc taattgtgag ataactgttg
     6241 ggaaaaatag acatggatcg gttttctctt cggtattaca tttcgatcca aaaattagta
     6301 aattctccgc tattaaaaaa gtatggtaaa ttatagtaac tgccacttca tcaaaagtcc
     6361 tatccacctt gaaaatcaga agtttggaag aagacctggt caatctatta agatatctcc
     6421 caaattggct caaaatggga tggtagaagt tataggtctt gattttcttt catctcatta
     6481 ccatgcatta gcagctatcc aaagattgct gactgcaacg aattacaagg ggaacacaaa
     6541 aggggttgtt ttatccagag aatcaaatag ttttcaattt gaaggatgga taccaagaat
     6601 ccgttttaca aaaactgaat tcttagaggc ttatggagtt aagcggtata aaacatccag
     6661 aaataagtat gagtttagtg gaaaagaagc tgaaactgct ttagaagcct tataccattt
     6721 aggacatcaa ccgtttttaa tagtggcaac tagaactcga tggactaatg gaacacaaat
     6781 agtagaccgt taccaaactc tttctccgat cattaggatt tacgaaggat gggaaggttt
     6841 aactgacgaa gaaaatatag atatagactt aacacctttt aattcaccat ctacacggaa
     6901 acataaaggg ttcgttgtag agccatgtcc tatcttggta gatcaaatag aatcctactt
     6961 tgtaatcaag cctgcaaatg tataccaaga aataaaaatg cgcttcccaa atgcatcaaa
     7021 gtatgcttac acatttatcg actgggtgat tacagcagct gcgaaaaaga gacgaaaatt
     7081 aactaaggat aattcttggc cagaaaactt gttcttaaac gttaacgtta aaagtcttgc
     7141 atatatttta aggatgaatc ggtacatttg tacaaggaac tggaaaaaaa tcgagttagc
     7201 tatcgataaa tgtatagaaa tcgccattca gcttggttgg ttatctagaa gaaaacgcat
     7261 tgaatttctg gattcttcta aactctctaa aaaagaaatt ctatatctaa ataaagagcg
     7321 ttttgaagaa ataactaaga aatctaaaga acaaatggaa caattagaac aagaatctat
     7381 taattaatag caaacttgaa actaaaaacc taatttattt aaagctcaaa ataaaaaaga
     7441 gttttaaaat gggaaattct ggtttttatt tgtataacac tcaaaactgc gtctttgctg
     7501 ataatatcaa agttgggcaa atgacagagc cgctcaagga ccagcaaata atccttggga
     7561 caacatcaac acctgtcgca gccaaaatga cagcttctga tggaatatct ttaacagtct
     7621 ccaataatcc atcaaccaat gcttctatta caattggttt ggatgcggaa aaagcttacc
     7681 agcttattct agaaaagttg ggagatcaaa ttcttggtgg aattgctgat actattgttg
     7741 atagtacagt ccaagatatt ttagacaaaa tcacaacaga cccttctcta ggtttgttga
     7801 aagcttttaa caactttcca atcactaata aaattcaatg caacgggtta ttcactccca
     7861 ggaacattga aactttatta ggaggaactg aaataggaaa attcacagtc acacccaaaa
     7921 gctctgggag catgttctta gtctcagcag atattattgc atcaagaatg gaaggcggcg
     7981 ttgttctagc tttggtacga gaaggtgatt ctaagcccta cgcgattagt tatggatact
     8041 catcaggcgt tcctaattta tgtagtctaa gaaccagaat tattaataca ggattgactc
     8101 cgacaacgta ttcattacgt gtaggcggtt tagaaagcgg tgtggtatgg gttaatgccc
     8161 tttctaatgg caatgatatt ttaggaataa caaatacttc taatgtatct tttttggagg
     8221 taatacctca aacaaacgct taaacaattt ttattggatt tttcttatag gttttatatt
     8281 tagagaaaaa agttcgaatt acggggtttg ttatgcaaaa taaaagcaaa gtgagggacg
     8341 attttattaa aattgttaaa gatgtgaaaa aagatttccc cgaattagac ctaaaaatac
     8401 gagtaaacaa ggaaaaagta actttcttaa attctccctt agaactctac cataaaagtg
     8461 tctcactaat tctaggactg cttcaacaaa tagaaaactc tttaggatta ttcccagact
     8521 ctcctgttct tgaaaaatta gaggataaca gtttaaagct aaaaaaggct ttgattatgc
     8581 ttatcttgtc tagaaaagac atgttttcca aggctgaata gataacttac tctaacgttg
     8641 gagttgattt gcacacctta gttttttgct cttttaaggg aggaactgga aaaacaacac
     8701 tttctctaaa cgtgggatgc aacttggccc aatttttagg gaaaaaagtg ttacttgctg
     8761 acctagaccc gcaatccaat ttatcttctg gattgggggc tagtgtcaga agtaaccaaa
     8821 aaggcttaca cgacatagta tacacatcaa acgatttaaa atcaatcatt tgcgaaacaa
     8881 aaaaagatag tgtggaccta attcctgcat catttttatc cgaacagttt agagaattgg
     8941 atattcatag aggacctagt aacaacttaa agttatttct gaatgagtac tgcgctcctt
     9001 tttatgacat ctgcataata gacactccac ctagcctagg agggttaacg aaagaagctt
     9061 ttgttgcagg agacaaatta attgcttgtt taactccaga acctttttct attctagggt
     9121 tacaaaagat acgtgaattc ttaagttcgg tcggaaaacc tgaagaagaa cacattcttg
     9181 gaatagcttt gtctttttgg gatgatcgta actcgactaa ccaaatgtat atagacatta
     9241 tcgagtctat ttacaaaaac aagctttttt caacaaaaat tcgtcgagat atttctctca
     9301 gccgttctct tcttaaagaa gattctgtag ctaatgtcta tccaaattct agggccgcag
     9361 aagatattct gaagttaacg catgaaatag caaatatttt gcatatcgaa tatgaacgag
     9421 attactctca gaggacaacg tgaacaaact aaaaaaagaa gcgaatgtct tttttaaaaa
     9481 aaatcaaact gccgcttctt tagattttaa gaagacgctt ccttccattg aactattctc
     9541 agcaactttg aattctgagg aaagtcagag tttggatcaa ttatttttat cagagtccca
     9601 aaactattcg gatgaagaat tttatcaaga agacatccta gcggtaaaac tgcttactgg
     9661 tcagataaaa tccatacaga agcaacacgt acttctttta ggagaaaaaa tctataatgc
     9721 tagaaaaatc ctgagtaagg atcacttctc ctcaacaact ttttcatctt ggatagagtt
     9781 agtttttaga actaagtctt ctgcttacaa tgctcttgca tattacgagc tttttataaa
     9841 cctccccaac caaactctac aaaaagagtt tcaatcgatc ccctataaat ccgcatatat
     9901 tttggccgct agaaaaggcg atttaaaaac caaggtcgat gtgataggga aagtatgtgg
     9961 aatgtcgaac tcatcggcga taagggtgtt ggatcaattt cttccttcat ctagaaacaa
    10021 agacgttaga gaaacgatag ataagtctga ttcagagaag aatcgccaat tatctgattt
    10081 cttaatagag atacttcgca tcatgtgttc cggagtctct ttgccctcct ataacgaaaa
    10141 tcttctacaa cagctttttg aactttttaa gcaaaagagc tgatcctccg tcagctcata
    10201 tatatatcta ttatatatat atatttaggg atttgatttt acgagagaga tttgcaactc
    10261 ttggtggtag actttgcaac tcttggtggt agactttgca actcttggtg gtagactttg
    10321 caactcttgg tggtagactt ggtcataatg gacttttgtt gaaaaatttc ttaaaatctt
    10381 agagctccga ttttgaatag ctttggttaa gaaaatgggc tcgatggctt tccataaaag
    10441 taggttgttc ttaacttttg gggacgcgtc ggaaatttgg ttatctactt tatctcatct
    10501 aactagaaaa aattatgcgt ctgggattaa ctttcttgtt tctttagaga ttctggattt
    10561 atcggaaacc ttgataaagg ctatttctct tgaccacagc gaatctttgt ttaaaatcaa
    10621 gtctctagat gtttttaatg gaaaagtcgt ttcagaggcc tctaaacagg ctagagcggc
    10681 atgctacata tctttcacaa agtttttgta tagattgacc aagggatata ttaaacccgc
    10741 tattccattg aaagattttg gaaacactac attttttaaa atccgagaca aaatcaaaac
    10801 agaatcgatt tctaagcagg aatggacagt tttttttgaa gcgctccgga tagtgaatta
    10861 tagagactat ttaatcggta aattgattgt acaagggatc cgtaagttag acgaaatttt
    10921 gtctttgcgc acagacgatc tattttttgc atccaatcag atttcctttc gcattaaaaa
    10981 aagacagaat aaagaaacca aaattctaat cacatttcct atcagcttaa tggaggagtt
    11041 gcaaaaatac acttgtggga gaaatgggag agtatttgtt tctaaaatag ggattcctgt
    11101 aacaacaagt caggttgcgc ataattttag gcttgcagag ttctatagtg ctatgaaaaa
    11161 aaaattactc ctagagtact tcgtgcaagc gctttgattc atttaaagca aataggatta
    11221 aaagatgagg aaatcatgcg tatttcctgt ctttcatcga gacaaagtgt gtgttcttat
    11281 tgttctgggg ggcgccaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    11341 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgcc
//
