LOCUS       sequence-224754-       11727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lux-reporter vector, integration at sacA, ampr, cmr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 11727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS3Clux
FEATURES             Location/Qualifiers
     source          1..11727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    123..144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(159..164,165..182,183..189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             231..242
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1058..1085
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1092..1112
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             1164..2246
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGVTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     RBS             2248..2256
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2263..3246
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAAADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
     CDS             3270..4712
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             4726..5649
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVALRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             5674..6786
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQAELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     complement(7602..7621)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7721..7743
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7781..7799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7867..7971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7972..8040,8041..8832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8190..8209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9003..9591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9492..9511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9745..9762
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10593..10612
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgatggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat atactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcctag
     1141 taaggtcgac aggaggactc tctatgaaat ttggaaactt tttgcttaca taccaacctc
     1201 cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg
     1261 agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg
     1321 gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa
     1381 ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat
     1441 tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg
     1501 actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg
     1561 ggctgataaa gaatggcgtg acagagggat atatggaagc tgataatgaa catatcaagt
     1621 tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg
     1681 tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa
     1741 gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc
     1801 aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata acatctgtag
     1861 atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt
     1921 cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt tatgatttca
     1981 ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat cgccgtattg
     2041 attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac ataattcaaa
     2101 aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct aatggaacag
     2161 tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca tttcttaaag
     2221 aaaaacaacg ttcgctatta tattagctaa ggaggtaaag aaatgaaatt tggattgttc
     2281 ttccttaact tcatcaattc aacaactgtt caagaacaaa gtatagttcg catgcaggaa
     2341 ataacggagt atgttgataa gttgaatttt gaacagattt tagtgtatga aaatcatttt
     2401 tcagataatg gtgttgtcgg cgctcctctg actgtttctg gttttctgct cggtttaaca
     2461 gagaaaatta aaattggttc attaaatcac atcattacaa ctcatcatcc tgtccgcata
     2521 gcggaggaag cttgcttatt ggatcagtta agtgaaggga gatttatttt agggtttagt
     2581 gattgcgaaa aaaaagatga aatgcatttt tttaatcgcc cggttgaata tcaacagcaa
     2641 ctatttgaag agtgttatga aatcattaac gatgctttaa caacaggcta ttgtaatcca
     2701 gataacgatt tttatagctt ccctaaaata tctgtaaatc cccatgctta tacgccaggc
     2761 ggacctcgga aatatgtaac agcaaccagt catcatattg ttgagtgggc ggccaaaaaa
     2821 ggtattcctc tcatctttaa gtgggatgat tctaatgatg ttagatatga atatgctgaa
     2881 agatataaag ccgctgcgga taaatatgac gttgacctat cagagataga ccatcagtta
     2941 atgatattag ttaactataa cgaagatagt aataaagcta aacaagagac gcgtgcattt
     3001 attagtgatt atgttcttga aatgcaccct aatgaaaatt tcgaaaataa acttgaagaa
     3061 ataattgcag aaaacgctgt cggaaattat acggagtgta taactgcggc taagttggca
     3121 attgaaaagt gtggtgcgaa aagtgtattg ctgtcctttg aaccaatgaa tgatttgatg
     3181 agccaaaaaa atgtaatcaa tattgttgat gataatatta agaagtacca catgaaatat
     3241 acctaatagg taccaggagg aaggcaaata tgactaaaaa aatttcattc attattaacg
     3301 gccaggttga aatctttccc gaaggtgatg atttagtgca atccattaat tttggtgata
     3361 atagtgttta cctgccaata ttgaatgact ctcatgtaaa aaacattatt gattgtaatg
     3421 gaaataacga attacggttg cataacattg tcaattttct ctatacggta gggcaaagat
     3481 ggaaaaatga agaatactca agacgcagga catacattcg tgacttaaaa aaatatatgg
     3541 gatattcaga agaaatggct aagctagagg ccaattggat atctatgatt ttatgttcta
     3601 aaggcggcct ttatgatgtt gtagaaaatg aacttggttc tcgccatatc atggatgaat
     3661 ggctacctca ggatgaaagt tatgttcggg cttttccgaa aggtaaatct gtacatctgt
     3721 tggcaggtaa tgttccatta tctgggatca tgtctatatt acgcgcaatt ttaactaaga
     3781 atcagtgtat tataaaaaca tcgtcaaccg atccttttac cgctaatgca ttagcgttaa
     3841 gttttattga tgtagaccct aatcatccga taacgcgctc tttatctgtt atatattggc
     3901 cccaccaagg tgatacatca ctcgcaaaag aaattatgca acatgcggat gttattgtcg
     3961 cttggggagg gccagatgcg attaattggg cggtagagca tgcgccatct tatgctgatg
     4021 tgattaaatt tggttctaaa aagagtcttt gcattatcga taatcctgtt gatttgacgt
     4081 ccgcagcgac aggtgcggct catgatgttt gtttttacga tcagcgagct tgtttttctg
     4141 cccaaaacat atattacatg ggaaatcatt atgaggaatt taagttagcg ttgatagaaa
     4201 aacttaatct atatgcgcat atattaccga atgccaaaaa agattttgat gaaaaggcgg
     4261 cctattcttt agttcaaaaa gaaagcttgt ttgctggatt aaaagtagag gtggatattc
     4321 atcaacgttg gatgattatt gagtcaaatg caggtgtgga atttaatcaa ccacttggca
     4381 gatgtgtgta ccttcatcac gtcgataata ttgagcaaat attgccttat gttcaaaaaa
     4441 ataagacgca aaccatatct atttttcctt gggagtcatc atttaaatat cgagatgcgt
     4501 tagcattaaa aggtgcggaa aggattgtag aagcaggaat gaataacata tttcgagttg
     4561 gtggatctca tgacggaatg agaccgttgc aacgattagt gacatatatt tctcatgaaa
     4621 ggccatctaa ctatacggct aaggatgttg cggttgaaat agaacagact cgattcctgg
     4681 aagaagataa gttccttgta tttgtcccat aatggaggta aaagtatgga aaatgaatca
     4741 aaatataaaa ccatcgacca cgttatttgt gttgaaggaa ataagaaaat tcatgtttgg
     4801 gaaacgctgc cagaagaaaa cagcccaaag agaaagaatg ccattattat tgcgtctggt
     4861 tttgcccgca ggatggatca ttttgctggt ctggcggaat atttatcgcg gaatggattt
     4921 catgtgatcc gctatgattc gcttcaccac gttggattga gttcagggac aattgatgaa
     4981 tttacaatgt ctataggaaa gcagagcttg ttagcagtgg ttgattggtt aactacacga
     5041 aaaataaata acttcggtat gttggcttca agcttatctg cgcggatagc ttatgcaagc
     5101 ctatctgaaa tcaatgcttc gtttttaatc accgcagtcg gtgttgttaa cttaagatat
     5161 tctcttgaaa gagctttagg gtttgattat ctcagtctac ccattaatga attgccggat
     5221 aatcttgatt ttgaaggcca taaattgggt gctgaagtct ttgcgagaga ttgtcttgat
     5281 tttggttggg aagatttagc ttctacaatt aataacatga tgtatcttga tataccgttt
     5341 attgctttta ctgcaaataa cgataattgg gtcaagcaag atgaagttat cacattgtta
     5401 tcaaatattc gtagtaatcg atgcaagata tattctttgt taggaagttc gcatgacttg
     5461 agtgaaaatt tagtggccct gcgcaatttt tatcaatcgg ttacgaaagc cgctatcgcg
     5521 atggataatg atcatctgga tattgatgtt gatattactg aaccgtcatt tgaacattta
     5581 actattgcga cagtcaatga acgccgaatg agaattgaga ttgaaaatca agcaatttct
     5641 ctgtcttaaa gaatcctgag gaggaaaaca ggtatgactt catatgttga taaacaagaa
     5701 attacagcaa gctcagaaat tgatgatttg attttttcga gcgatccatt agtgtggtct
     5761 tacgacgagc aggaaaaaat cagaaagaaa cttgtgcttg atgcatttcg taatcattat
     5821 aaacattgtc gagaatatcg tcactactgt caggcacaca aagtagatga caatattacg
     5881 gaaattgatg acatacctgt attcccaaca tcggttttta agtttactcg cttattaact
     5941 tctcaggaaa acgagattga aagttggttt accagtagcg gcacgaatgg tttaaaaagt
     6001 caggtggcgc gtgacagatt aagtattgag agactcttag gctctgtgag ttatggcatg
     6061 aaatatgttg gtagttggtt tgatcatcaa atagaattag tcaatttggg accagataga
     6121 tttaatgctc ataatatttg gtttaaatat gttatgagtt tggtggaatt gttatatcct
     6181 acgacattta ccgtaacaga agaacgaata gattttgtta aaacattgaa tagtcttgaa
     6241 cgaataaaaa atcaagggaa agatctttgt cttattggtt cgccatactt tatttattta
     6301 ctctgccatt atatgaaaga taaaaaaatc tcattttctg gagataaaag cctttatatc
     6361 ataaccggag gcggctggaa aagttacgaa aaagaatctc tgaaacgtga tgatttcaat
     6421 catcttttat ttgatacttt caatctcagt gatattagtc agatccgaga tatatttaat
     6481 caagctgaac tcaacacttg tttctttgag gatgaaatgc agcgtaaaca tgttccgccg
     6541 tgggtatatg cgcgagcgct tgatcctgaa acgttgaaac ctgtacctga tggaacgccg
     6601 gggttgatga gttatatgga tgcgtcagca accagttatc cagcatttat tgttaccgat
     6661 gatgtcggga taattagcag agaatatggt aagtatcccg gcgtgctcgt tgaaatttta
     6721 cgtcgcgtca atacgaggac gcagaaaggg tgtgctttaa gcttaaccga agcgtttgat
     6781 agttgatatc cagcaggatc ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
     6841 aaaaataata ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta
     6901 tgtactattt aattctgcgt gacatcccat cgatcagacc agtttttaat ttgtgtgttt
     6961 ccatgtgtcc agtttggcgc gccccatccg accattgact gccactggat tcactgcatg
     7021 acgctgccga gacagctgac gctttcagga cagaagctga ttcagcagcc gctgcctgag
     7081 ctaaaagcca tgcgcagaaa tgagaaaaaa atacacatca acatgcatgg atcatctggt
     7141 gcgcttccag tggaaaaacc tgaaagaact gagattctac tggaagacat tcatacggag
     7201 tctggctttt caatcagtat ccgcggaacg gctacgtttt ccttccataa agacgagggg
     7261 attgttacgc tggaacgaaa gagctttgac ggaaaaagaa cagaagcgag acattgccgc
     7321 atcaaggatt tgcataccgt acacatgttt ctcgacgcgt catctgtgga aatctttatc
     7381 aataacggag aagaggtctt tagtgcaaga tattttcctt tcccgggaaa tcatgaagta
     7441 acagccagtg cgaccgggaa atcctggcgt aatagcgaag aggcccgcac cgatcgccct
     7501 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     7561 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     7621 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7681 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7741 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7801 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7861 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7921 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8101 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8161 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8221 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8281 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8341 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8401 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8461 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8521 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8881 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8941 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9001 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9061 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9121 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9181 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9241 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9301 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9361 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9421 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9481 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9541 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9601 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9661 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9721 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9781 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcaggac agcacatgac
     9841 caggagcttc gtcgccgggc ttatgaagaa gtggagaaaa aagagcccat cgctaacagc
     9901 gatccgcacc gccagcattt tcatatcatg ccgccggttg ggctgctgaa tgacccgaat
     9961 ggcgtgattt attggaaggg cagctatcat gtattctttc agtggcagcc gtttcagacg
    10021 gggcacggcg caaaattttg ggggcattat acgacacagg atgttgtgaa ttggaagcgg
    10081 gaagagattg cgctggctcc gagtgattgg tttgataaaa acggctgcta ctcgggcagc
    10141 gctgtcacga aagacgatcg gctctatctt ttttacacag gaaatgtcag ggatcaggat
    10201 ggaaatcggg aaacgtatca atgccttgct gtttctgacg acgggctgtc ctttgagaaa
    10261 aagggtgtcg tcgcccgcct tccggaagga tatacggcgc attttcgcga tccgaaggta
    10321 tgggagcatg aaggcacatg gtatatggtg attggtgcgc aaacagagaa tttgaaaggg
    10381 caggctgtgt tgtttgcttc tgataacctg acagagtgga gatttcttgg cccgataacc
    10441 ggcgcgggct tcaacgggct ggacgatttt ggcgcgccta aacggatcaa ggagatggcg
    10501 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
    10561 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
    10621 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggagc
    10681 tgtaatataa aaaccttctt caactaacgg ggcaggttag tgacattaga aaaccgactg
    10741 taaaaagtac agtcggcatt atctcatatt ataaaagcca gtcattaggc ctatctgaca
    10801 attcctgaat agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta
    10861 cctgtaaaga tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa
    10921 taatgggtag aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag
    10981 tccatggaat aatagaaaga gaaaaagcat tttcaggtat aggtgttttg ggaaacaatt
    11041 tccccgaacc attatatttc tctacatcag aaaggtataa atcataaaac tctttgaagt
    11101 cattctttac aggagtccaa ataccagaga atgttttaga tacaccatca aaaattgtat
    11161 aaagtggctc taacttatcc caataaccta actctccgtc gctattgtaa ccagttctaa
    11221 aagctgtatt tgagtttatc acccttgtca ctaagaaaat aaatgcaggg taaaatttat
    11281 atccttcttg ttttatgttt cggtataaaa cactaatatc aatttctgtg gttatactaa
    11341 aagtcgtttg ttggttcaaa taatgattaa atatctcttt tctcttccaa ttgtctaaat
    11401 caattttatt aaagttcatt tgatatgcct cctaaatttt tatctaaagt gaatttagga
    11461 ggcttacttg tctgctttct tcattagaat caatcctttt ttaaaagtca atattactgt
    11521 aacataaata tatattttaa aaatatccca ctttatccaa ttttcgtttg ttgaactaat
    11581 gggtgcttta gttgaagaat aaaagaccac attaaaaaat gtggtctttt gtgttttttt
    11641 aaaggatttg agcgtagcga aaaatccttt tctttcttat cttgataata agggtaacta
    11701 ttgccgatga taagctgtca aacatga
//
