LOCUS       sequence-224765-        9581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab21
FEATURES             Location/Qualifiers
     source          1..9581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1405..1425)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1433..1449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1465..1599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1489..1508)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1543..1562
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2038..2225,2226..2237)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2271..2287)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2271..2287)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2324..2344)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2324..2342)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2363..2379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2376..2398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2387..2403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2411..2417,2418..2435,2436..2441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2456..2477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2594..2611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2765..3353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2845..2864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3524..4315,4316..4384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4147..4166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4385..4489)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4652..4673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5022..5033,5034..5196)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5569..5585
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5605..5625
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcggcg gctggaggga agacgtactc
      781 gttcaaggtg gtgctgctgg gcgagggttg cgtcgggaaa acatccctgg ttctgcgata
      841 ctgcgagaac aaattcaacg acaaacacat caccacgctt caggcatcgt tcctcacaaa
      901 gaagctcaac ataacaggaa agagagtaaa tctggctata tgggatacgg ctggtcagga
      961 gcgttttcat gcccttgggc ccatctacta cagagattcc aacggcgcga tattggtgta
     1021 cgacgtcaca gatgaggact cttttcagaa ggtgaagaac tgggtgaaag aattaaggaa
     1081 aatgttgggg aatgaaatct gtttatgtat agtaggcaat aaaatagacc ttgaaaaaga
     1141 gcggcatgtt tcagtcgagg aagcagaggg gtatgcagag tcagttggag cgaaacacta
     1201 tcacacatcc gccaaattaa acaaaggcat cgaggagctg tttctggacc tgtgtaaacg
     1261 catgatggag acagctcagc tggaggagcg gtcgaggggg aacggagcca gtaacagcag
     1321 cacaggccgg aggggtgtac agattgtgga tgatgagccg caggccgccg tcccagccgg
     1381 aggctgttgc acatccggct aagccaactt tattatacat agttgataat tcactggccg
     1441 tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga tgagtttgga
     1501 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     1561 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     1621 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     1681 aaatgtggta tggctgatta tgatcctcta gatcagatct gcgaagatac ggccacgggt
     1741 gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat gaatcacata
     1801 cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc aggtcaaggt
     1861 gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca atgaacatgt
     1921 ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata acatttaaat
     1981 taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa tagaatgaag
     2041 tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag taaaaagtac
     2101 ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt aattgtactc
     2161 aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta aaattactca
     2221 agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca gatccactag
     2281 ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     2341 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2401 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2461 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2521 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2581 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2641 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2701 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2761 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2821 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2881 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2941 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3001 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3061 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taaggcattt
     3421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3541 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4501 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4561 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4621 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4681 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccacgctg
     4741 gctaagaact catcagcctc cccggtccat ctacccacgt accaatgcac caattggcca
     4801 caatgacggc tactacatgg tgccattcct tcctctttat aggaatggag actacctcct
     4861 gtccaacaat gctcttggat acgagtacgc ctacctgttg gacccaggtc attgcacaac
     4921 accagaaatg ccctctgatc tgcaaaagac gtgaatatct gttcagacac ccatatccac
     4981 tctgttccac acaggtcaga ggtttgtcca ggagttcttg acagaggtgt aaaaagtact
     5041 caaaaatttt actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt
     5101 aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt
     5161 acttgagtat taaggaagta aaagtaaaag caagaaagaa aactagagat tcttgtttaa
     5221 gcttttaatc tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct gctttagaac
     5281 tgtttgtatt taattatcaa actataagac agacaatcta atgccagtac acgctactca
     5341 aagttgtaaa acctcagatt taacttcagt agaagctgat tctcaaaatt gttagtgtca
     5401 agcctagctc ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca
     5461 gccgatgcgg gaagaggtgt attagtcttg atagagaggc tgcaaatagc aggaaacgtg
     5521 agcagagact ccctggtgtc tgaaacacag gccagatggg ccctcgagca ggaaacagct
     5581 atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttggcacg cgcagattcc
     5641 gtgtgggcct ggtaatatct taccatttta agctatttta agattaggga aaaaaattaa
     5701 ataaaactac tttaaaatta ctttccttaa aaagcaacat atttatttag tttttgttta
     5761 actggctcaa gattgtaata ttttactttt acatgttttt ttaagtacct aaatgtgttc
     5821 aaaagtaaag tatgttattg tttttttttt ttaatgttag ctaagcttta aattgtaata
     5881 tattactttt aaaagtgact ttttaaaaat gtaaataaaa aagtaactat aaatgtgtaa
     5941 tgcatgttat ttctgtgggt ttttaatttt catctcaact gggcttacaa ttttaacata
     6001 ataaaaatca aatcaaatca aatcaggccc ttatttgtca catacactct tgtatactga
     6061 aataaactaa atgttgagaa aaatttaaat acaccaaaat aacgcatacc ttttttgtaa
     6121 ttacctaagt aaaacattta gttccttttt taagtgactc aagattgtaa tctattaatt
     6181 gtaaaagata aaactagtga tcaatctgac gtgcttctat gcagcctgtg agattaattg
     6241 tgatgtttaa ttattaaaca atttaaatgg atcaaacatt tcacatgtgc ttattgtgtc
     6301 caacaaaacc agcagaagca cttaaaaata ttgcatgaca acatgagttt ttaatgtaaa
     6361 ttatatatac ttcctaaacg aagttaatca tatttctaaa ataataatgt cattgaaata
     6421 tttaaataaa tgataagctt tagttgattc aacataaaat tgaaagcaat aaggactttt
     6481 tttttttaca gcggtgaagc aaaagcatgt aataatcata taatataata aataaataaa
     6541 taaataatgg ctcttggtgc aaatatgaac actttaaata aatatagaat aaatatgatc
     6601 aataagtcat aacgatgtgt tttatgttga ttataattta aacatgtttc taacgcaagt
     6661 tttaaaaact gttttaaagt attattttaa gttataagat caatttgact caacattttg
     6721 tagtgaaaat atgttgtgta aaattaatat aatatacact ataaaaatgc agggttccac
     6781 acaatacatt aatgttgtcc caacacaaat cgattaagtt aacaaatata tgtggattga
     6841 acataaaaaa ttaagttgtc ccaatgaaat ctcaagaatt gtgttgtttc agctcatttt
     6901 aaataagtag ttttaacaag taaaaaataa catatttttg agtgtgtata tatatatatt
     6961 acaaaatata ataatataaa atgtaaaaga tataaaccat gctacatata tttaagagct
     7021 attttgcaca ttactttttt gcaagccatt ttagtgtttt ttttacaaat atatattagc
     7081 taaatataca atatcttaaa tatacaatta atcagacatg caattaatgc atttaaccta
     7141 cataaaaata catgatcaac atatgtttta tgctgattac aactaaatca aacgtaatga
     7201 aattaattta taaaagcgat ttaaatatgt caatatttta agttaaaatc acttgaaacc
     7261 tactataaat gaacttaaat taaaaaaatc tcctttaatt ccaatgcaac agtgttgttt
     7321 tttcagctga ggacccccgg tgcagatggt tggcccccgg cccgctattc taaggaagcc
     7381 tttcttaatt ttagggcggc gctgcatgcg ctcagatcag attcacgggt ggagagggtt
     7441 attttctctt ttttattttt tcttgcctgc tcatatttgg gcctgtgtga accccaggct
     7501 ccgcctcggc ccccaggagc cgctcttatt taaaggggat cctcgccttc agtcttcgtc
     7561 ccagtggaag catcagtctt gtgataacaa cttggtgagt cctacagacc agcgctcgac
     7621 tctaggcttt aaatgcattg atttctctgg attagctacc gggagagttt taactttgct
     7681 tattagtctt gtgctcgaac tgctaggttt aaagagtgtt ttctctaatg catgcatgaa
     7741 gcgacagctg aaaaccagaa atagagcgtt tgtcctcctg ttaaactcta gcctttcttt
     7801 ggcatttgtc agtccgagtc ttcagctgcc ggcaggagat tgggtttcag taacacttgc
     7861 tgagcacaaa ctacacgtgc tttcttcaga tttaattcat tattaatcat tctagattta
     7921 aatgatgttc ttactcattg ctgattattt tagccttgcg gagcagatta aatgagcacg
     7981 tgtttcatta agattgattt aaatataatt aatatttcac cactgtagtt ttaaaattgc
     8041 cttctaagtc atggttgatt tttaataggt atcggctcta attagattac tattttggtt
     8101 tttaaatgtt atttctattt catttgtagg ctcgttttta atgtgatttt attagattct
     8161 agttttaaaa tcatctctta gccttaatga gcagactgaa tgaacatgtt tcattaagat
     8221 taacttcatt ataatgaatt atttaatcat tctagtgggt tataaatcag ctctatttaa
     8281 attaattgtc gtgtttattt ctatttaatt tttgattatt ttttcagggt cgtttttatg
     8341 tgattttttc ccgtttctaa ttttaaacta attgtttgat ttttatatga ttcgactctt
     8401 tttaaatgaa ttgttttgct ttttttattg agcatatata tattatacaa tttcaatcaa
     8461 caattgttgt gctttttaaa tgttgtttct atttcatttc tagggtcgat tttattcgat
     8521 tctagtttta aaatcgtgat ttttaacttc taaccttact gagcagactg aatgaacatg
     8581 tttcatcaag attaacttca ttatagttaa ttattgaatc gtgctagtgg tttataaatc
     8641 agctctattt aaattaatca tcatcattat ttctatttaa tttttattat ttctttagga
     8701 ttatttttat gtgatttttt tcacgttcta gttttaaact caatgctcat tactgctcac
     8761 ccttgctgag cagtctttcg ttaagattaa attaattaat aaccattcta gttttaaaac
     8821 agtgtcttaa ggcatagctg atattttaca agtttactat ttaatatatc tcctttcttt
     8881 ttttagggtc gatttcatct gatttgatta atttttatta tcttcactaa gctttttgtt
     8941 ctaggtttta ttctcagtat tattactgtt atatttgaac tgaatttctc ctcatagttt
     9001 attcatttta tttatttatt ttttgtttat tcgctatttt gtattttagt ttttaattag
     9061 aaattttatt agaatcgttt tgaactagtt tttatgcact ctaacattaa tattaaatca
     9121 taactattat ttttattata atttatttgt gtttatcttt ttgtgtagct ttcatgtgtt
     9181 tttattttag ttttattatg tttgcattga attttttgga ctagtttata taacgatata
     9241 cagaaaagcc atgacaacaa atgtttttat aatacctatt aagtataagc taatcagatt
     9301 tccactattc tgttcaaagt taacattatt agccattctg atggatcatt tgacatttgt
     9361 catatgtctt tatgaacact gctctttcag atgccacttt taaagctgtt ttttacagta
     9421 aatgaacgtt tattattatg acattatttc ctaaggcctc tagagtacag caggatgtgg
     9481 cgtgtgcatt accgtgtaat tatgctgact gtaaatgtgc atttatagac tgtcagcatg
     9541 tctgactgtg cgctgttgtg attggtcctg cacagaccaa g
//
