LOCUS       sequence-224768-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab23
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1466..1486)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1494..1510)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1526..1660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1550..1569)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1604..1623
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2099..2286,2287..2298)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2332..2348)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2332..2348)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2385..2405)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2385..2403)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2424..2440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2437..2459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2448..2464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2472..2478,2479..2496,2497..2502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2517..2538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2655..2672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2826..3414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2906..2925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3585..4376,4377..4445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4208..4227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4446..4550)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4713..4734)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5083..5094,5095..5257)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5630..5646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5666..5686
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgctggag gaggacatgg aggtggccat
      781 caaggtggtg gtggtgggaa acggagcggt gggcaagtcc agcatgatcc agaggtactg
      841 caagggcatc ttcaccaagg actacaagaa gaccatcggc gtggactttc tggagaggca
      901 gatcatagtg aacggcgagg acgtcagact gatgttgtgg gacacggcgg ggcaggagga
      961 gtttgatgcc atcactaaag cttattacag aggagctcag gcctgtgtgc tggttttctc
     1021 caccaccgac agagagtcat tcgaggccat cagcagctgg aaggagaagg tggagatgga
     1081 ggtgggcgac attcccactg tcctggtgca gaacaaaatc gacctgctgg acgacaccgt
     1141 catcaagaat gaagaggctg aaggtcttgc taaaaggctg aagttgagat tctaccggac
     1201 gtcagtgaag gaggatctga atgtcaatga agtttttaag tatctagctg acaaatatct
     1261 gcagcgactc aagcagcagt cagcggagga gacggatatc attcacacat ccagcaataa
     1321 aataggcgtc ttcaacacaa caggcagcgg tcatccaaac cagaacagca gcgatttgaa
     1381 tggccgagag atcgtaaacc tgcgaccaaa caagcagcgg acgaagaaaa cgaaaaaccc
     1441 cttcggcagc tgcagactcc tgtagcaact ttattataca tagttgataa ttcactggcc
     1501 gtcgttttac ggtaccatcg atgatgatcc agacatgata agatacattg atgagtttgg
     1561 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1621 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1681 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     1741 caaatgtggt atggctgatt atgatcctct agatcagatc tgcgaagata cggccacggg
     1801 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
     1861 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
     1921 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
     1981 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
     2041 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
     2101 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     2161 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     2221 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     2281 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
     2341 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     2401 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2461 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2521 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2581 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2641 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3241 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaaggcatt
     3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4561 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4621 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4681 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4741 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccacgct
     4801 ggctaagaac tcatcagcct ccccggtcca tctacccacg taccaatgca ccaattggcc
     4861 acaatgacgg ctactacatg gtgccattcc ttcctcttta taggaatgga gactacctcc
     4921 tgtccaacaa tgctcttgga tacgagtacg cctacctgtt ggacccaggt cattgcacaa
     4981 caccagaaat gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca
     5041 ctctgttcca cacaggtcag aggtttgtcc aggagttctt gacagaggtg taaaaagtac
     5101 tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag
     5161 taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg
     5221 tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta
     5281 agcttttaat ctcaaaaaac attaaatgaa atgcatacaa ggttttatcc tgctttagaa
     5341 ctgtttgtat ttaattatca aactataaga cagacaatct aatgccagta cacgctactc
     5401 aaagttgtaa aacctcagat ttaacttcag tagaagctga ttctcaaaat tgttagtgtc
     5461 aagcctagct cttttggggc tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc
     5521 agccgatgcg ggaagaggtg tattagtctt gatagagagg ctgcaaatag caggaaacgt
     5581 gagcagagac tccctggtgt ctgaaacaca ggccagatgg gccctcgagc aggaaacagc
     5641 tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttggcac gcgcagattc
     5701 cgtgtgggcc tggtaatatc ttaccatttt aagctatttt aagattaggg aaaaaaatta
     5761 aataaaacta ctttaaaatt actttcctta aaaagcaaca tatttattta gtttttgttt
     5821 aactggctca agattgtaat attttacttt tacatgtttt tttaagtacc taaatgtgtt
     5881 caaaagtaaa gtatgttatt gttttttttt tttaatgtta gctaagcttt aaattgtaat
     5941 atattacttt taaaagtgac tttttaaaaa tgtaaataaa aaagtaacta taaatgtgta
     6001 atgcatgtta tttctgtggg tttttaattt tcatctcaac tgggcttaca attttaacat
     6061 aataaaaatc aaatcaaatc aaatcaggcc cttatttgtc acatacactc ttgtatactg
     6121 aaataaacta aatgttgaga aaaatttaaa tacaccaaaa taacgcatac cttttttgta
     6181 attacctaag taaaacattt agttcctttt ttaagtgact caagattgta atctattaat
     6241 tgtaaaagat aaaactagtg atcaatctga cgtgcttcta tgcagcctgt gagattaatt
     6301 gtgatgttta attattaaac aatttaaatg gatcaaacat ttcacatgtg cttattgtgt
     6361 ccaacaaaac cagcagaagc acttaaaaat attgcatgac aacatgagtt tttaatgtaa
     6421 attatatata cttcctaaac gaagttaatc atatttctaa aataataatg tcattgaaat
     6481 atttaaataa atgataagct ttagttgatt caacataaaa ttgaaagcaa taaggacttt
     6541 ttttttttac agcggtgaag caaaagcatg taataatcat ataatataat aaataaataa
     6601 ataaataatg gctcttggtg caaatatgaa cactttaaat aaatatagaa taaatatgat
     6661 caataagtca taacgatgtg ttttatgttg attataattt aaacatgttt ctaacgcaag
     6721 ttttaaaaac tgttttaaag tattatttta agttataaga tcaatttgac tcaacatttt
     6781 gtagtgaaaa tatgttgtgt aaaattaata taatatacac tataaaaatg cagggttcca
     6841 cacaatacat taatgttgtc ccaacacaaa tcgattaagt taacaaatat atgtggattg
     6901 aacataaaaa attaagttgt cccaatgaaa tctcaagaat tgtgttgttt cagctcattt
     6961 taaataagta gttttaacaa gtaaaaaata acatattttt gagtgtgtat atatatatat
     7021 tacaaaatat aataatataa aatgtaaaag atataaacca tgctacatat atttaagagc
     7081 tattttgcac attacttttt tgcaagccat tttagtgttt tttttacaaa tatatattag
     7141 ctaaatatac aatatcttaa atatacaatt aatcagacat gcaattaatg catttaacct
     7201 acataaaaat acatgatcaa catatgtttt atgctgatta caactaaatc aaacgtaatg
     7261 aaattaattt ataaaagcga tttaaatatg tcaatatttt aagttaaaat cacttgaaac
     7321 ctactataaa tgaacttaaa ttaaaaaaat ctcctttaat tccaatgcaa cagtgttgtt
     7381 ttttcagctg aggacccccg gtgcagatgg ttggcccccg gcccgctatt ctaaggaagc
     7441 ctttcttaat tttagggcgg cgctgcatgc gctcagatca gattcacggg tggagagggt
     7501 tattttctct tttttatttt ttcttgcctg ctcatatttg ggcctgtgtg aaccccaggc
     7561 tccgcctcgg cccccaggag ccgctcttat ttaaagggga tcctcgcctt cagtcttcgt
     7621 cccagtggaa gcatcagtct tgtgataaca acttggtgag tcctacagac cagcgctcga
     7681 ctctaggctt taaatgcatt gatttctctg gattagctac cgggagagtt ttaactttgc
     7741 ttattagtct tgtgctcgaa ctgctaggtt taaagagtgt tttctctaat gcatgcatga
     7801 agcgacagct gaaaaccaga aatagagcgt ttgtcctcct gttaaactct agcctttctt
     7861 tggcatttgt cagtccgagt cttcagctgc cggcaggaga ttgggtttca gtaacacttg
     7921 ctgagcacaa actacacgtg ctttcttcag atttaattca ttattaatca ttctagattt
     7981 aaatgatgtt cttactcatt gctgattatt ttagccttgc ggagcagatt aaatgagcac
     8041 gtgtttcatt aagattgatt taaatataat taatatttca ccactgtagt tttaaaattg
     8101 ccttctaagt catggttgat ttttaatagg tatcggctct aattagatta ctattttggt
     8161 ttttaaatgt tatttctatt tcatttgtag gctcgttttt aatgtgattt tattagattc
     8221 tagttttaaa atcatctctt agccttaatg agcagactga atgaacatgt ttcattaaga
     8281 ttaacttcat tataatgaat tatttaatca ttctagtggg ttataaatca gctctattta
     8341 aattaattgt cgtgtttatt tctatttaat ttttgattat tttttcaggg tcgtttttat
     8401 gtgatttttt cccgtttcta attttaaact aattgtttga tttttatatg attcgactct
     8461 ttttaaatga attgttttgc tttttttatt gagcatatat atattataca atttcaatca
     8521 acaattgttg tgctttttaa atgttgtttc tatttcattt ctagggtcga ttttattcga
     8581 ttctagtttt aaaatcgtga tttttaactt ctaaccttac tgagcagact gaatgaacat
     8641 gtttcatcaa gattaacttc attatagtta attattgaat cgtgctagtg gtttataaat
     8701 cagctctatt taaattaatc atcatcatta tttctattta atttttatta tttctttagg
     8761 attattttta tgtgattttt ttcacgttct agttttaaac tcaatgctca ttactgctca
     8821 cccttgctga gcagtctttc gttaagatta aattaattaa taaccattct agttttaaaa
     8881 cagtgtctta aggcatagct gatattttac aagtttacta tttaatatat ctcctttctt
     8941 tttttagggt cgatttcatc tgatttgatt aatttttatt atcttcacta agctttttgt
     9001 tctaggtttt attctcagta ttattactgt tatatttgaa ctgaatttct cctcatagtt
     9061 tattcatttt atttatttat tttttgttta ttcgctattt tgtattttag tttttaatta
     9121 gaaattttat tagaatcgtt ttgaactagt ttttatgcac tctaacatta atattaaatc
     9181 ataactatta tttttattat aatttatttg tgtttatctt tttgtgtagc tttcatgtgt
     9241 ttttatttta gttttattat gtttgcattg aattttttgg actagtttat ataacgatat
     9301 acagaaaagc catgacaaca aatgttttta taatacctat taagtataag ctaatcagat
     9361 ttccactatt ctgttcaaag ttaacattat tagccattct gatggatcat ttgacatttg
     9421 tcatatgtct ttatgaacac tgctctttca gatgccactt ttaaagctgt tttttacagt
     9481 aaatgaacgt ttattattat gacattattt cctaaggcct ctagagtaca gcaggatgtg
     9541 gcgtgtgcat taccgtgtaa ttatgctgac tgtaaatgtg catttataga ctgtcagcat
     9601 gtctgactgt gcgctgttgt gattggtcct gcacagacca ag
//
