LOCUS       sequence-224773-        9779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab40c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab40c
FEATURES             Location/Qualifiers
     source          1..9779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1603..1623)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1631..1647)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1663..1797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1687..1706)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1741..1760
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2236..2423,2424..2435)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2469..2485)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2469..2485)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2522..2542)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2522..2540)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2561..2577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2574..2596)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2585..2601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2609..2615,2616..2633,2634..2639))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2654..2675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2792..2809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2963..3551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3043..3062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3722..4513,4514..4582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4345..4364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4583..4687)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4850..4871)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5220..5231,5232..5394)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5767..5783
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5803..5823
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgggcacc cagggaagcc ctgtgaagag
      781 ctacgactac ctgctcaagt tcctcctggt cggagacagc gacgtgggca agggagagat
      841 cctggatagc cttcaagacg gatctgctga gtctccatat gcctacagca gcggaatcga
      901 ctacaagaca accacgatcc tgttggatgg acggagagtt aaactagagc tttgggacac
      961 ttcgggacaa ggcaggtttt gcaccatttt caggtcgtac tccaggggag cgcaggggat
     1021 cttgttggtg tatgacatca ccaaccgctg gtcatttgat gggatagacc gctggatcag
     1081 ggagattgat gagcatgcac ctggagtgcc ccgtattcta gtgggtaacc ggctacacct
     1141 ggcgttcaag cgacaggttc ccacagaaca ggcacgggca tacgcagaaa agaatggcat
     1201 gacatttttt gaagtgagtc ctctctgcaa cttcaacgtc atcgagtcct ttacagagct
     1261 ctcgcgcatc gtcctgatga ggcatgggat ggagaagttc tggaggccca acagagtatt
     1321 cagtctgcag gacttgtgct gccgtgccat tgtttcctgc acccctgtgc acctaatcga
     1381 taagctgcca cttcctgtgg ccatcaagag ccatttgaag tcattctcca tggcgaacgg
     1441 catgaatgct gtcatgatgc acggccgctc gtattcgctg gccaatgccg ccggaggcag
     1501 caaaggtaac agtcttaaac gctccaaatc catccggccg ccacaaagtc caccacagaa
     1561 ctgcaccagg aacaactgta agatctccta gcctccaggc accaacttta ttatacatag
     1621 ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga catgataaga
     1681 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1741 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1801 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1861 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc
     1921 gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag
     1981 ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt ataatttccc
     2041 taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca
     2101 aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag
     2161 aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca
     2221 agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt
     2281 gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat
     2341 tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta
     2401 atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa
     2461 gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
     2521 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3601 actcacgtta aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4681 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     4741 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4801 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4861 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4921 gggcgatggc ccacgctggc taagaactca tcagcctccc cggtccatct acccacgtac
     4981 caatgcacca attggccaca atgacggcta ctacatggtg ccattccttc ctctttatag
     5041 gaatggagac tacctcctgt ccaacaatgc tcttggatac gagtacgcct acctgttgga
     5101 cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt gaatatctgt
     5161 tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg agttcttgac
     5221 agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa
     5281 ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa
     5341 gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa
     5401 ctagagattc ttgtttaagc ttttaatctc aaaaaacatt aaatgaaatg catacaaggt
     5461 tttatcctgc tttagaactg tttgtattta attatcaaac tataagacag acaatctaat
     5521 gccagtacac gctactcaaa gttgtaaaac ctcagattta acttcagtag aagctgattc
     5581 tcaaaattgt tagtgtcaag cctagctctt ttggggctga aaagcaatcc tgcagtgctg
     5641 aaaagcctct cacaggcagc cgatgcggga agaggtgtat tagtcttgat agagaggctg
     5701 caaatagcag gaaacgtgag cagagactcc ctggtgtctg aaacacaggc cagatgggcc
     5761 ctcgagcagg aaacagctat gaccatgatt acgccaagct atcaactttg tatagaaaag
     5821 ttggcacgcg cagattccgt gtgggcctgg taatatctta ccattttaag ctattttaag
     5881 attagggaaa aaaattaaat aaaactactt taaaattact ttccttaaaa agcaacatat
     5941 ttatttagtt tttgtttaac tggctcaaga ttgtaatatt ttacttttac atgttttttt
     6001 aagtacctaa atgtgttcaa aagtaaagta tgttattgtt tttttttttt aatgttagct
     6061 aagctttaaa ttgtaatata ttacttttaa aagtgacttt ttaaaaatgt aaataaaaaa
     6121 gtaactataa atgtgtaatg catgttattt ctgtgggttt ttaattttca tctcaactgg
     6181 gcttacaatt ttaacataat aaaaatcaaa tcaaatcaaa tcaggccctt atttgtcaca
     6241 tacactcttg tatactgaaa taaactaaat gttgagaaaa atttaaatac accaaaataa
     6301 cgcatacctt ttttgtaatt acctaagtaa aacatttagt tcctttttta agtgactcaa
     6361 gattgtaatc tattaattgt aaaagataaa actagtgatc aatctgacgt gcttctatgc
     6421 agcctgtgag attaattgtg atgtttaatt attaaacaat ttaaatggat caaacatttc
     6481 acatgtgctt attgtgtcca acaaaaccag cagaagcact taaaaatatt gcatgacaac
     6541 atgagttttt aatgtaaatt atatatactt cctaaacgaa gttaatcata tttctaaaat
     6601 aataatgtca ttgaaatatt taaataaatg ataagcttta gttgattcaa cataaaattg
     6661 aaagcaataa ggactttttt tttttacagc ggtgaagcaa aagcatgtaa taatcatata
     6721 atataataaa taaataaata aataatggct cttggtgcaa atatgaacac tttaaataaa
     6781 tatagaataa atatgatcaa taagtcataa cgatgtgttt tatgttgatt ataatttaaa
     6841 catgtttcta acgcaagttt taaaaactgt tttaaagtat tattttaagt tataagatca
     6901 atttgactca acattttgta gtgaaaatat gttgtgtaaa attaatataa tatacactat
     6961 aaaaatgcag ggttccacac aatacattaa tgttgtccca acacaaatcg attaagttaa
     7021 caaatatatg tggattgaac ataaaaaatt aagttgtccc aatgaaatct caagaattgt
     7081 gttgtttcag ctcattttaa ataagtagtt ttaacaagta aaaaataaca tatttttgag
     7141 tgtgtatata tatatattac aaaatataat aatataaaat gtaaaagata taaaccatgc
     7201 tacatatatt taagagctat tttgcacatt acttttttgc aagccatttt agtgtttttt
     7261 ttacaaatat atattagcta aatatacaat atcttaaata tacaattaat cagacatgca
     7321 attaatgcat ttaacctaca taaaaataca tgatcaacat atgttttatg ctgattacaa
     7381 ctaaatcaaa cgtaatgaaa ttaatttata aaagcgattt aaatatgtca atattttaag
     7441 ttaaaatcac ttgaaaccta ctataaatga acttaaatta aaaaaatctc ctttaattcc
     7501 aatgcaacag tgttgttttt tcagctgagg acccccggtg cagatggttg gcccccggcc
     7561 cgctattcta aggaagcctt tcttaatttt agggcggcgc tgcatgcgct cagatcagat
     7621 tcacgggtgg agagggttat tttctctttt ttattttttc ttgcctgctc atatttgggc
     7681 ctgtgtgaac cccaggctcc gcctcggccc ccaggagccg ctcttattta aaggggatcc
     7741 tcgccttcag tcttcgtccc agtggaagca tcagtcttgt gataacaact tggtgagtcc
     7801 tacagaccag cgctcgactc taggctttaa atgcattgat ttctctggat tagctaccgg
     7861 gagagtttta actttgctta ttagtcttgt gctcgaactg ctaggtttaa agagtgtttt
     7921 ctctaatgca tgcatgaagc gacagctgaa aaccagaaat agagcgtttg tcctcctgtt
     7981 aaactctagc ctttctttgg catttgtcag tccgagtctt cagctgccgg caggagattg
     8041 ggtttcagta acacttgctg agcacaaact acacgtgctt tcttcagatt taattcatta
     8101 ttaatcattc tagatttaaa tgatgttctt actcattgct gattatttta gccttgcgga
     8161 gcagattaaa tgagcacgtg tttcattaag attgatttaa atataattaa tatttcacca
     8221 ctgtagtttt aaaattgcct tctaagtcat ggttgatttt taataggtat cggctctaat
     8281 tagattacta ttttggtttt taaatgttat ttctatttca tttgtaggct cgtttttaat
     8341 gtgattttat tagattctag ttttaaaatc atctcttagc cttaatgagc agactgaatg
     8401 aacatgtttc attaagatta acttcattat aatgaattat ttaatcattc tagtgggtta
     8461 taaatcagct ctatttaaat taattgtcgt gtttatttct atttaatttt tgattatttt
     8521 ttcagggtcg tttttatgtg attttttccc gtttctaatt ttaaactaat tgtttgattt
     8581 ttatatgatt cgactctttt taaatgaatt gttttgcttt ttttattgag catatatata
     8641 ttatacaatt tcaatcaaca attgttgtgc tttttaaatg ttgtttctat ttcatttcta
     8701 gggtcgattt tattcgattc tagttttaaa atcgtgattt ttaacttcta accttactga
     8761 gcagactgaa tgaacatgtt tcatcaagat taacttcatt atagttaatt attgaatcgt
     8821 gctagtggtt tataaatcag ctctatttaa attaatcatc atcattattt ctatttaatt
     8881 tttattattt ctttaggatt atttttatgt gatttttttc acgttctagt tttaaactca
     8941 atgctcatta ctgctcaccc ttgctgagca gtctttcgtt aagattaaat taattaataa
     9001 ccattctagt tttaaaacag tgtcttaagg catagctgat attttacaag tttactattt
     9061 aatatatctc ctttcttttt ttagggtcga tttcatctga tttgattaat ttttattatc
     9121 ttcactaagc tttttgttct aggttttatt ctcagtatta ttactgttat atttgaactg
     9181 aatttctcct catagtttat tcattttatt tatttatttt ttgtttattc gctattttgt
     9241 attttagttt ttaattagaa attttattag aatcgttttg aactagtttt tatgcactct
     9301 aacattaata ttaaatcata actattattt ttattataat ttatttgtgt ttatcttttt
     9361 gtgtagcttt catgtgtttt tattttagtt ttattatgtt tgcattgaat tttttggact
     9421 agtttatata acgatataca gaaaagccat gacaacaaat gtttttataa tacctattaa
     9481 gtataagcta atcagatttc cactattctg ttcaaagtta acattattag ccattctgat
     9541 ggatcatttg acatttgtca tatgtcttta tgaacactgc tctttcagat gccactttta
     9601 aagctgtttt ttacagtaaa tgaacgttta ttattatgac attatttcct aaggcctcta
     9661 gagtacagca ggatgtggcg tgtgcattac cgtgtaatta tgctgactgt aaatgtgcat
     9721 ttatagactg tcagcatgtc tgactgtgcg ctgttgtgat tggtcctgca cagaccaag
//
