LOCUS       sequence-224774-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab21
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(777..872)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(909..928)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(910..928)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(933..949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(933..949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1051..1070)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1062..1871
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1129..1148)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2018..2606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2507..2526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2760..2777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2936..2963)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3055..3141)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3190..3212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3204..3221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3205..3221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggcgg cggctggagg gaagacgtac tcgttcaagg tggtgctgct gggcgagggt
      181 tgcgtcggga aaacatccct ggttctgcga tactgcgaga acaaattcaa cgacaaacac
      241 atcaccacgc ttcaggcatc gttcctcaca aagaagctca acataacagg aaagagagta
      301 aatctggcta tatgggatac ggctggtcag gagcgttttc atgcccttgg gcccatctac
      361 tacagagatt ccaacggcgc gatattggtg tacgacgtca cagatgagga ctcttttcag
      421 aaggtgaaga actgggtgaa agaattaagg aaaatgttgg ggaatgaaat ctgtttatgt
      481 atagtaggca ataaaataga ccttgaaaaa gagcggcatg tttcagtcga ggaagcagag
      541 gggtatgcag agtcagttgg agcgaaacac tatcacacat ccgccaaatt aaacaaaggc
      601 atcgaggagc tgtttctgga cctgtgtaaa cgcatgatgg agacagctca gctggaggag
      661 cggtcgaggg ggaacggagc cagtaacagc agcacaggcc ggaggggtgt acagattgtg
      721 gatgatgagc cgcaggccgc cgtcccagcc ggaggctgtt gcacatccgg ctaagccaac
      781 tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca
      841 ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt taacgcggcc
      901 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      961 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     1021 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1081 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     1141 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     1201 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     1261 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     1321 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     1381 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     1441 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     1501 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     1561 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     1621 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1681 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     1741 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     1801 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     1861 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     1921 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     1981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2821 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2881 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     2941 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3001 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3061 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3121 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     3181 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccctgca
     3241 gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt t
//
