LOCUS       sequence-224775-        9582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab11a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab11a
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1406..1426)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1434..1450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1466..1600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1490..1509)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1544..1563
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2039..2226,2227..2238)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2272..2288)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2288)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2325..2345)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2325..2343)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2364..2380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2377..2399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2388..2404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2412..2418,2419..2436,2437..2442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2457..2478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2595..2612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2766..3354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2846..2865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3525..4316,4317..4385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4148..4167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4386..4490)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4653..4674)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5023..5034,5035..5197)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5570..5586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5606..5626
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtcaccatg gggacacgag acgacgaata
      781 cgactacttg tttaaagtgg tcctaattgg agactctggt gtggggaaga gtaacctgct
      841 gtctcgtttc actcgcaatg aattcaacct cgagagcaaa agcactattg gagtggagtt
      901 cgccacacgt agcattcagg tggatggaaa gaccgtcaag gctcagatct gggacacggc
      961 aggacaggag cgctaccgag ccatcacttc agcgtattac cgtggagctg tgggggccct
     1021 cctagtgtat gacatcgcca agcatctgac ctatgagaat gtggaacgct ggcttaagga
     1081 gctccgagac cacgcagaca gcaacattgt catcatgctg gtgggcaata aaagtgactt
     1141 gcgtcacctt cgggctgtgc ccactgacga agcacgtgca tttgcagaga aaaacggtct
     1201 gtccttccta gagacctcag ctttggattc caccaatgta gagactgctt ttcagaccat
     1261 cctgactgaa atctaccgga tcgtatccca aaagcagatg tctgaccgcc gagacaatga
     1321 catgtcacct agcaacaatg tggtgtccat ccaggtgcaa cctactgaga ataaaccaaa
     1381 gatgcagtgc tgccagagca tctagcaact ttattataca tagttgataa ttcactggcc
     1441 gtcgttttac ggtaccatcg atgatgatcc agacatgata agatacattg atgagtttgg
     1501 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1561 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1621 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     1681 caaatgtggt atggctgatt atgatcctct agatcagatc tgcgaagata cggccacggg
     1741 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
     1801 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
     1861 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
     1921 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
     1981 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
     2041 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     2101 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     2161 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     2221 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
     2281 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     2341 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2401 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2461 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2521 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2581 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2641 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3181 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3361 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaaggcatt
     3421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3481 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3541 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3601 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3661 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3721 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3781 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3841 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3901 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3961 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4021 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4081 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4141 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4201 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4261 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4321 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4381 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4441 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4501 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4561 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4621 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4681 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccacgct
     4741 ggctaagaac tcatcagcct ccccggtcca tctacccacg taccaatgca ccaattggcc
     4801 acaatgacgg ctactacatg gtgccattcc ttcctcttta taggaatgga gactacctcc
     4861 tgtccaacaa tgctcttgga tacgagtacg cctacctgtt ggacccaggt cattgcacaa
     4921 caccagaaat gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca
     4981 ctctgttcca cacaggtcag aggtttgtcc aggagttctt gacagaggtg taaaaagtac
     5041 tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag
     5101 taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg
     5161 tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta
     5221 agcttttaat ctcaaaaaac attaaatgaa atgcatacaa ggttttatcc tgctttagaa
     5281 ctgtttgtat ttaattatca aactataaga cagacaatct aatgccagta cacgctactc
     5341 aaagttgtaa aacctcagat ttaacttcag tagaagctga ttctcaaaat tgttagtgtc
     5401 aagcctagct cttttggggc tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc
     5461 agccgatgcg ggaagaggtg tattagtctt gatagagagg ctgcaaatag caggaaacgt
     5521 gagcagagac tccctggtgt ctgaaacaca ggccagatgg gccctcgagc aggaaacagc
     5581 tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttggcac gcgcagattc
     5641 cgtgtgggcc tggtaatatc ttaccatttt aagctatttt aagattaggg aaaaaaatta
     5701 aataaaacta ctttaaaatt actttcctta aaaagcaaca tatttattta gtttttgttt
     5761 aactggctca agattgtaat attttacttt tacatgtttt tttaagtacc taaatgtgtt
     5821 caaaagtaaa gtatgttatt gttttttttt tttaatgtta gctaagcttt aaattgtaat
     5881 atattacttt taaaagtgac tttttaaaaa tgtaaataaa aaagtaacta taaatgtgta
     5941 atgcatgtta tttctgtggg tttttaattt tcatctcaac tgggcttaca attttaacat
     6001 aataaaaatc aaatcaaatc aaatcaggcc cttatttgtc acatacactc ttgtatactg
     6061 aaataaacta aatgttgaga aaaatttaaa tacaccaaaa taacgcatac cttttttgta
     6121 attacctaag taaaacattt agttcctttt ttaagtgact caagattgta atctattaat
     6181 tgtaaaagat aaaactagtg atcaatctga cgtgcttcta tgcagcctgt gagattaatt
     6241 gtgatgttta attattaaac aatttaaatg gatcaaacat ttcacatgtg cttattgtgt
     6301 ccaacaaaac cagcagaagc acttaaaaat attgcatgac aacatgagtt tttaatgtaa
     6361 attatatata cttcctaaac gaagttaatc atatttctaa aataataatg tcattgaaat
     6421 atttaaataa atgataagct ttagttgatt caacataaaa ttgaaagcaa taaggacttt
     6481 ttttttttac agcggtgaag caaaagcatg taataatcat ataatataat aaataaataa
     6541 ataaataatg gctcttggtg caaatatgaa cactttaaat aaatatagaa taaatatgat
     6601 caataagtca taacgatgtg ttttatgttg attataattt aaacatgttt ctaacgcaag
     6661 ttttaaaaac tgttttaaag tattatttta agttataaga tcaatttgac tcaacatttt
     6721 gtagtgaaaa tatgttgtgt aaaattaata taatatacac tataaaaatg cagggttcca
     6781 cacaatacat taatgttgtc ccaacacaaa tcgattaagt taacaaatat atgtggattg
     6841 aacataaaaa attaagttgt cccaatgaaa tctcaagaat tgtgttgttt cagctcattt
     6901 taaataagta gttttaacaa gtaaaaaata acatattttt gagtgtgtat atatatatat
     6961 tacaaaatat aataatataa aatgtaaaag atataaacca tgctacatat atttaagagc
     7021 tattttgcac attacttttt tgcaagccat tttagtgttt tttttacaaa tatatattag
     7081 ctaaatatac aatatcttaa atatacaatt aatcagacat gcaattaatg catttaacct
     7141 acataaaaat acatgatcaa catatgtttt atgctgatta caactaaatc aaacgtaatg
     7201 aaattaattt ataaaagcga tttaaatatg tcaatatttt aagttaaaat cacttgaaac
     7261 ctactataaa tgaacttaaa ttaaaaaaat ctcctttaat tccaatgcaa cagtgttgtt
     7321 ttttcagctg aggacccccg gtgcagatgg ttggcccccg gcccgctatt ctaaggaagc
     7381 ctttcttaat tttagggcgg cgctgcatgc gctcagatca gattcacggg tggagagggt
     7441 tattttctct tttttatttt ttcttgcctg ctcatatttg ggcctgtgtg aaccccaggc
     7501 tccgcctcgg cccccaggag ccgctcttat ttaaagggga tcctcgcctt cagtcttcgt
     7561 cccagtggaa gcatcagtct tgtgataaca acttggtgag tcctacagac cagcgctcga
     7621 ctctaggctt taaatgcatt gatttctctg gattagctac cgggagagtt ttaactttgc
     7681 ttattagtct tgtgctcgaa ctgctaggtt taaagagtgt tttctctaat gcatgcatga
     7741 agcgacagct gaaaaccaga aatagagcgt ttgtcctcct gttaaactct agcctttctt
     7801 tggcatttgt cagtccgagt cttcagctgc cggcaggaga ttgggtttca gtaacacttg
     7861 ctgagcacaa actacacgtg ctttcttcag atttaattca ttattaatca ttctagattt
     7921 aaatgatgtt cttactcatt gctgattatt ttagccttgc ggagcagatt aaatgagcac
     7981 gtgtttcatt aagattgatt taaatataat taatatttca ccactgtagt tttaaaattg
     8041 ccttctaagt catggttgat ttttaatagg tatcggctct aattagatta ctattttggt
     8101 ttttaaatgt tatttctatt tcatttgtag gctcgttttt aatgtgattt tattagattc
     8161 tagttttaaa atcatctctt agccttaatg agcagactga atgaacatgt ttcattaaga
     8221 ttaacttcat tataatgaat tatttaatca ttctagtggg ttataaatca gctctattta
     8281 aattaattgt cgtgtttatt tctatttaat ttttgattat tttttcaggg tcgtttttat
     8341 gtgatttttt cccgtttcta attttaaact aattgtttga tttttatatg attcgactct
     8401 ttttaaatga attgttttgc tttttttatt gagcatatat atattataca atttcaatca
     8461 acaattgttg tgctttttaa atgttgtttc tatttcattt ctagggtcga ttttattcga
     8521 ttctagtttt aaaatcgtga tttttaactt ctaaccttac tgagcagact gaatgaacat
     8581 gtttcatcaa gattaacttc attatagtta attattgaat cgtgctagtg gtttataaat
     8641 cagctctatt taaattaatc atcatcatta tttctattta atttttatta tttctttagg
     8701 attattttta tgtgattttt ttcacgttct agttttaaac tcaatgctca ttactgctca
     8761 cccttgctga gcagtctttc gttaagatta aattaattaa taaccattct agttttaaaa
     8821 cagtgtctta aggcatagct gatattttac aagtttacta tttaatatat ctcctttctt
     8881 tttttagggt cgatttcatc tgatttgatt aatttttatt atcttcacta agctttttgt
     8941 tctaggtttt attctcagta ttattactgt tatatttgaa ctgaatttct cctcatagtt
     9001 tattcatttt atttatttat tttttgttta ttcgctattt tgtattttag tttttaatta
     9061 gaaattttat tagaatcgtt ttgaactagt ttttatgcac tctaacatta atattaaatc
     9121 ataactatta tttttattat aatttatttg tgtttatctt tttgtgtagc tttcatgtgt
     9181 ttttatttta gttttattat gtttgcattg aattttttgg actagtttat ataacgatat
     9241 acagaaaagc catgacaaca aatgttttta taatacctat taagtataag ctaatcagat
     9301 ttccactatt ctgttcaaag ttaacattat tagccattct gatggatcat ttgacatttg
     9361 tcatatgtct ttatgaacac tgctctttca gatgccactt ttaaagctgt tttttacagt
     9421 aaatgaacgt ttattattat gacattattt cctaaggcct ctagagtaca gcaggatgtg
     9481 gcgtgtgcat taccgtgtaa ttatgctgac tgtaaatgtg catttataga ctgtcagcat
     9541 gtctgactgt gcgctgttgt gattggtcct gcacagacca ag
//
