LOCUS       sequence-224778-        9636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab12
FEATURES             Location/Qualifiers
     source          1..9636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1460..1480)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1488..1504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1520..1654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1544..1563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1598..1617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2093..2280,2281..2292)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2326..2342)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2326..2342)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2379..2399)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2379..2397)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2418..2434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2431..2453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2442..2458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2466..2472,2473..2490,2491..2496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2511..2532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2649..2666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2820..3408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2900..2919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3579..4370,4371..4439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4202..4221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4440..4544)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4707..4728)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5077..5088,5089..5251)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5624..5640
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5660..5680
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggctgagtcg ggcttcgggg tccaacggag
      781 ggccggtgga ggctctcccg ctgtgggagc ttcgcagcgc cgcaggaaga ttccgcccag
      841 agccgctgat tataaactgc aggtgatcat tattggctcc agaggtgtgg ggaaaaccag
      901 cctgatggag agattcacgg atgacacttt ttgtgaggcg tgcaagtcca ctgtgggtgt
      961 ggattttaaa atcaagacag tggagctgag aggaaagaag atcaggttgc agatctggga
     1021 cacggcaggt caggagcggt ttaacagcat cacttcagcg tattaccgcg gtgccaaagg
     1081 cattgttttg gtttatgaca tcaccaagca ggaaactttt gaagacctgc ctaaatggat
     1141 gaagatgata gacaagtatg cgtcagagga tgctgagctc ctattggttg ggaacaaact
     1201 ggactgcgag tctgatcgcg ccatctcacg gcaacaagct gagaggtttg cttcacggat
     1261 atcagggatg cgtttctgtg aggccagtgc caaggataac tttaacgtgg atgagatctt
     1321 ccttaaacta gttgatgaca tcttgagtaa gatgccactt gaagtcccca gtaaggagct
     1381 ttccaacagt gttttgtctc ttcaacccga gcctgaaatc ccaccagagc tgcctccgcc
     1441 acgcatgcgc tgctgttgac aactttatta tacatagttg ataattcact ggccgtcgtt
     1501 ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac
     1561 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1621 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1681 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1741 tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct
     1801 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
     1861 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
     1921 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
     1981 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
     2041 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
     2101 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     2161 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     2221 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     2281 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
     2341 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     2401 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2461 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2521 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2581 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2641 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2701 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2821 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3241 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc
     3481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4561 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4621 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4681 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4741 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca cgctggctaa
     4801 gaactcatca gcctccccgg tccatctacc cacgtaccaa tgcaccaatt ggccacaatg
     4861 acggctacta catggtgcca ttccttcctc tttataggaa tggagactac ctcctgtcca
     4921 acaatgctct tggatacgag tacgcctacc tgttggaccc aggtcattgc acaacaccag
     4981 aaatgccctc tgatctgcaa aagacgtgaa tatctgttca gacacccata tccactctgt
     5041 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa
     5101 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag
     5161 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg
     5221 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt
     5281 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt
     5341 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt
     5401 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct
     5461 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga
     5521 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag
     5581 agactccctg gtgtctgaaa cacaggccag atgggccctc gagcaggaaa cagctatgac
     5641 catgattacg ccaagctatc aactttgtat agaaaagttg gcacgcgcag attccgtgtg
     5701 ggcctggtaa tatcttacca ttttaagcta ttttaagatt agggaaaaaa attaaataaa
     5761 actactttaa aattactttc cttaaaaagc aacatattta tttagttttt gtttaactgg
     5821 ctcaagattg taatatttta cttttacatg tttttttaag tacctaaatg tgttcaaaag
     5881 taaagtatgt tattgttttt tttttttaat gttagctaag ctttaaattg taatatatta
     5941 cttttaaaag tgacttttta aaaatgtaaa taaaaaagta actataaatg tgtaatgcat
     6001 gttatttctg tgggttttta attttcatct caactgggct tacaatttta acataataaa
     6061 aatcaaatca aatcaaatca ggcccttatt tgtcacatac actcttgtat actgaaataa
     6121 actaaatgtt gagaaaaatt taaatacacc aaaataacgc ataccttttt tgtaattacc
     6181 taagtaaaac atttagttcc ttttttaagt gactcaagat tgtaatctat taattgtaaa
     6241 agataaaact agtgatcaat ctgacgtgct tctatgcagc ctgtgagatt aattgtgatg
     6301 tttaattatt aaacaattta aatggatcaa acatttcaca tgtgcttatt gtgtccaaca
     6361 aaaccagcag aagcacttaa aaatattgca tgacaacatg agtttttaat gtaaattata
     6421 tatacttcct aaacgaagtt aatcatattt ctaaaataat aatgtcattg aaatatttaa
     6481 ataaatgata agctttagtt gattcaacat aaaattgaaa gcaataagga cttttttttt
     6541 ttacagcggt gaagcaaaag catgtaataa tcatataata taataaataa ataaataaat
     6601 aatggctctt ggtgcaaata tgaacacttt aaataaatat agaataaata tgatcaataa
     6661 gtcataacga tgtgttttat gttgattata atttaaacat gtttctaacg caagttttaa
     6721 aaactgtttt aaagtattat tttaagttat aagatcaatt tgactcaaca ttttgtagtg
     6781 aaaatatgtt gtgtaaaatt aatataatat acactataaa aatgcagggt tccacacaat
     6841 acattaatgt tgtcccaaca caaatcgatt aagttaacaa atatatgtgg attgaacata
     6901 aaaaattaag ttgtcccaat gaaatctcaa gaattgtgtt gtttcagctc attttaaata
     6961 agtagtttta acaagtaaaa aataacatat ttttgagtgt gtatatatat atattacaaa
     7021 atataataat ataaaatgta aaagatataa accatgctac atatatttaa gagctatttt
     7081 gcacattact tttttgcaag ccattttagt gtttttttta caaatatata ttagctaaat
     7141 atacaatatc ttaaatatac aattaatcag acatgcaatt aatgcattta acctacataa
     7201 aaatacatga tcaacatatg ttttatgctg attacaacta aatcaaacgt aatgaaatta
     7261 atttataaaa gcgatttaaa tatgtcaata ttttaagtta aaatcacttg aaacctacta
     7321 taaatgaact taaattaaaa aaatctcctt taattccaat gcaacagtgt tgttttttca
     7381 gctgaggacc cccggtgcag atggttggcc cccggcccgc tattctaagg aagcctttct
     7441 taattttagg gcggcgctgc atgcgctcag atcagattca cgggtggaga gggttatttt
     7501 ctctttttta ttttttcttg cctgctcata tttgggcctg tgtgaacccc aggctccgcc
     7561 tcggccccca ggagccgctc ttatttaaag gggatcctcg ccttcagtct tcgtcccagt
     7621 ggaagcatca gtcttgtgat aacaacttgg tgagtcctac agaccagcgc tcgactctag
     7681 gctttaaatg cattgatttc tctggattag ctaccgggag agttttaact ttgcttatta
     7741 gtcttgtgct cgaactgcta ggtttaaaga gtgttttctc taatgcatgc atgaagcgac
     7801 agctgaaaac cagaaataga gcgtttgtcc tcctgttaaa ctctagcctt tctttggcat
     7861 ttgtcagtcc gagtcttcag ctgccggcag gagattgggt ttcagtaaca cttgctgagc
     7921 acaaactaca cgtgctttct tcagatttaa ttcattatta atcattctag atttaaatga
     7981 tgttcttact cattgctgat tattttagcc ttgcggagca gattaaatga gcacgtgttt
     8041 cattaagatt gatttaaata taattaatat ttcaccactg tagttttaaa attgccttct
     8101 aagtcatggt tgatttttaa taggtatcgg ctctaattag attactattt tggtttttaa
     8161 atgttatttc tatttcattt gtaggctcgt ttttaatgtg attttattag attctagttt
     8221 taaaatcatc tcttagcctt aatgagcaga ctgaatgaac atgtttcatt aagattaact
     8281 tcattataat gaattattta atcattctag tgggttataa atcagctcta tttaaattaa
     8341 ttgtcgtgtt tatttctatt taatttttga ttattttttc agggtcgttt ttatgtgatt
     8401 ttttcccgtt tctaatttta aactaattgt ttgattttta tatgattcga ctctttttaa
     8461 atgaattgtt ttgctttttt tattgagcat atatatatta tacaatttca atcaacaatt
     8521 gttgtgcttt ttaaatgttg tttctatttc atttctaggg tcgattttat tcgattctag
     8581 ttttaaaatc gtgattttta acttctaacc ttactgagca gactgaatga acatgtttca
     8641 tcaagattaa cttcattata gttaattatt gaatcgtgct agtggtttat aaatcagctc
     8701 tatttaaatt aatcatcatc attatttcta tttaattttt attatttctt taggattatt
     8761 tttatgtgat ttttttcacg ttctagtttt aaactcaatg ctcattactg ctcacccttg
     8821 ctgagcagtc tttcgttaag attaaattaa ttaataacca ttctagtttt aaaacagtgt
     8881 cttaaggcat agctgatatt ttacaagttt actatttaat atatctcctt tcttttttta
     8941 gggtcgattt catctgattt gattaatttt tattatcttc actaagcttt ttgttctagg
     9001 ttttattctc agtattatta ctgttatatt tgaactgaat ttctcctcat agtttattca
     9061 ttttatttat ttattttttg tttattcgct attttgtatt ttagttttta attagaaatt
     9121 ttattagaat cgttttgaac tagtttttat gcactctaac attaatatta aatcataact
     9181 attattttta ttataattta tttgtgttta tctttttgtg tagctttcat gtgtttttat
     9241 tttagtttta ttatgtttgc attgaatttt ttggactagt ttatataacg atatacagaa
     9301 aagccatgac aacaaatgtt tttataatac ctattaagta taagctaatc agatttccac
     9361 tattctgttc aaagttaaca ttattagcca ttctgatgga tcatttgaca tttgtcatat
     9421 gtctttatga acactgctct ttcagatgcc acttttaaag ctgtttttta cagtaaatga
     9481 acgtttatta ttatgacatt atttcctaag gcctctagag tacagcagga tgtggcgtgt
     9541 gcattaccgt gtaattatgc tgactgtaaa tgtgcattta tagactgtca gcatgtctga
     9601 ctgtgcgctg ttgtgattgg tcctgcacag accaag
//
