LOCUS       sequence-224788-       14799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab44.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14799)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 14799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab44
FEATURES             Location/Qualifiers
     source          1..14799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6623..6643)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6651..6667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6683..6817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6707..6726)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6761..6780
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7256..7443,7444..7455)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7489..7505)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7489..7505)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7542..7562)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7542..7560)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7581..7597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7594..7616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7605..7621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7629..7635,7636..7653,7654..7659))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7674..7695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7812..7829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7983..8571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8063..8082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8742..9533,9534..9602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9365..9384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9603..9707)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9870..9891)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(10240..10251,10252..10414)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     10787..10803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10823..10843
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt gggtcgactg gaagaaggag gagaaaccaa
      781 ctcaattcct gaacaaagtg gcatcagtgt cattccaagc ccagttgttc agagaagaaa
      841 aataggctca actcgaagac ctctaaaagg gaataatgtg actgcagagt cagatgtgct
      901 tcaaaaagcc actttagctg atgcaaaatt agaaaaagac aatgtatctg aaaatactga
      961 gagcctttca gctgtcaccg attctaatct tttacttcaa catcatggca ctgaaggagc
     1021 aggagaaaca gactcaattc ctaaacaaag tggcatcagc atcatccctg gagatcttca
     1081 aagaaaggag acaaacttag agtcaagtcc agttattcag agaagaaaaa tgggctcaac
     1141 tcgaagacct ctcaaaggaa ataaaggaca aagaaaagga cgagagcttc atgatgaaaa
     1201 tgaaacattt aacagtgaat ttttgcttga tgtacagaaa gaaacattcg gtttggagga
     1261 tgaaccctgt ccagatgaat ctacattaca aaacataaaa gatggagctg aagaacagga
     1321 gaaggaactg tgtgtgaaga atgatgctag tcttgttgga gagtctgaat ctgctctcat
     1381 tcagtcatca tacggttcag atttagcgtc tggagaaacc actgcattga gtgtctgtaa
     1441 catcacagaa ccacaaaccc tcacaaaaac tcacgaagag gcctgggaaa tagttggggg
     1501 tgttgaaaga gcaacagtag aagaagacac agacttgcaa tcaaaaaccg agtcacaaaa
     1561 ccaaacatgt gcctcaatta gtgaagcaga agaaaacatc catgatgtaa atgttttagt
     1621 cagagaagct gaagttactg gagaatcagt tatattggat agtaatgtga ctgcagagtc
     1681 agatgtgatt caaaaagaca ctttagctga tgcagaatta gaaacattgc aagtttctga
     1741 aaatacagag gatgtttctg ctgtgactga tactgatttt ctagtgcaac atcatggcac
     1801 tgaacgagca gaagggacag acacaatttc tgtacaaagt ggcatcaaca tcattcctag
     1861 agagtctcac ggagaagagg cgaactcaaa gtcgagtcca gttgttcaga ggagaaaaat
     1921 gggctcaact cgaagacctc tcaaaggaaa taaaggacaa agaaaaggac aagagcttga
     1981 tgatgaaaag gaaacatttc actgtgaatt tttgcttgat gtagaaaaag aaacatttag
     2041 tttggagaat gaaccctgtc cagatgaatc tacattacaa aacatgaaag atggagctga
     2101 agaacaggag aaggaaatat gtctggagaa tgatgttggt cttgttgaag agtctgaatc
     2161 tgctctcatt cagtcatata gttcagattt agtgtctgaa gaatccactg cattgagtgt
     2221 ctgtaacatc acagaatcac aaaccctcac aaaaattcaa gaagagtcct gggaaatagt
     2281 tggagatgtt gaaagagaat cagtagatgg agatacagac ttacaattag aaacagagtc
     2341 acaaaaccaa acatgtgact caaccagtaa agcagaacaa aacatccatg atgtaaatgt
     2401 attagtcaga gaagctgaag gtattgggga atcagttaaa gtggacagca atgtgattgc
     2461 agagtcagat gtgcttcaaa aagccacttt agctgatcca aaattggaaa cattgcacgt
     2521 atctgaaaat actaaggatg tttctgctgt cacggattct gatcttctag tgcaacatga
     2581 accaactgta gaagcaggag aaacagacac ggttcctgaa aaaagtggaa tcagcatcat
     2641 tcctggagat tctcacaacg aagaggcaaa ctcaaagtca agtccagttg ttcagaggag
     2701 aaaaatgggc tcaactcgaa gacctctcaa aggaaataaa ggacaaagaa aaggacgaga
     2761 gcttcaagat gaaaatgaga cacttaataa tgaaattagg tttgatgtag agaaagaaac
     2821 atttggtttg gagaatgaac cctgtccaga tgaatctgca ttacaaaaca taaaagttgg
     2881 ggctgaagaa caggagaagg aaatatgtgt gaagaatgat gctggtgttg ttgaagagta
     2941 tgaatctgat ctcattcagt cattttatgg ttcggattta gtgtctgaag aatccactgc
     3001 attaagtgtc tgccacatcg aagaagcaca aactctcaca attagtcatg aacaatttca
     3061 gaataatcct ggagatgcag aaagagcagc agtagttgaa gatgctgccg atactgaaga
     3121 tacttcagaa actgaagcta cagttacaat gaagagcact acaaatactg catcacatgt
     3181 ttttcaaacg tctcctagtt caagcacaga tgcatttaat atatctacaa atgtcgagga
     3241 agatacttca ggagacacag acacaatttt caagcagagt aacatcaacg ttgttcatga
     3301 agatatttat agaggggaag ctcacccaaa gttaagtcca gttgttcaga agagaaaaat
     3361 gggctcaact cgaagacctc tcagggggaa caaaggacaa aaaaaagata atgatgaaaa
     3421 ggaaatctca tttctggaaa atgaacccaa tccagatgag cttatataca ctgcaatggg
     3481 tatcagtgat atttcagaag ctcaaaccca cgtaaaagtt cacgaagagt cccaggacaa
     3541 ccatgaagtt gttgaaaaag caacagtagg ggaaagcaca caatcacaaa cagaaaacaa
     3601 agtgagtgtg ttagtcagag aagctgaagt tcctgaaaat acagttgtga ttaatacaac
     3661 tgaggagtcg tatttgtttc aatacgtctc tgattcaaaa ctgaccatat ctgactatga
     3721 acatgaggct ccagtaattc caaacataat ttccaaacaa agtgacttca gcattcctgg
     3781 agattctcac aaagaagagg aaaactcaca attaagtcca gttgttcaaa agagaaaaat
     3841 gggctcaact cgaagacctc tcaaagggaa taaggggcaa ggaaagcaga gagaagaatc
     3901 caaggtaaag aatagagata atgaggcagg gtcagaagag gaaaacacag aaacacattc
     3961 aaaagaccag gaacaaaatg tggcaaaaga caatatgaat ataatagata atgtcaatga
     4021 agaaaaaaag gcaacaaagg cattggatac atccatgcag gaactatgtg aacaagagca
     4081 aagatcagat gtatcacaaa cacatgctga tacatcacag gatgaaacca gctcacattt
     4141 attggacatc ccagaaagaa ctgtagattt tgttagggag tctacgagtc agcctgatct
     4201 tgttctgtct gatgtgttgc ttatgacaga agctcaaacg tatttgtcat cagaagataa
     4261 tgtcatgggt aacagttcaa tatctataac agaggtacat gaaatgcatg cagactccaa
     4321 tacatccaag agaaaaatgg ggtcaactag aaagagagga aagaggatgg tgaatgaaga
     4381 ggaagaggaa gctgaaaata caattggttc tggagccaca agttccaagg agacattaac
     4441 tcctaaaaaa ttaattgcag aatcttctgt acataaacca ctgtttagtt catatgttat
     4501 taacgaagac catgacagta aatcagatgt gcagactgag ctctcagatc aagattatct
     4561 aaattctacc ataaacaccg aaccaatgaa agaaagagaa agttctccag atgttgttgc
     4621 tgaaatggtt tctgactttg ctttgggaca tgttgacact gaagtagttc ctgagcaaag
     4681 ggtcattcct gaagagccac ataaagatca agcaaacctg aatccagttg ttcaaaaacg
     4741 aaaaatgggg tcaacacgta aaactcttag agggaacaaa gggcagagaa aagaggaaga
     4801 atcgaaagaa attaatgaag gaatgacaca ggatcggagt agagaactac atttggcaag
     4861 tgaaagtaaa cttggtgaag aggttcaaca aaaccaatct cttattaata tgacaaacaa
     4921 taaagaagct aagaaaccgg aggatgaatt ggatgaaatg ccttcagcat caagcatctc
     4981 tcaattcaca gaagcatcct cagaaactct cagttcctca tatcttaaat cagaaaagtt
     5041 agattcaaaa gatgagaatg attatcatga tacttgtgaa catgaaaatg cccatcagat
     5101 ttgcgctgat gctaccaaaa gtctacagtc ggaatctgtt cccattgaga ggagaaggaa
     5161 gatgggctcg acccggaaaa atattggaag agaaggaaga atgcaaagaa agcgagatgg
     5221 atatgaggac acagaaatca tagacacaaa tctgcagatg tctcacgacc ctgaaatgag
     5281 ctcatcaggt ataactgcag aagaacaaac aactgaagat tactccaatc agtcagagga
     5341 ttcacttctt ggtgaaatac agcgaatgct aaaaacaact tctcaaagtg catccaattt
     5401 tgaattgatt attgatgagc cgaaatcaaa ttcaccagaa ggctcagaaa tcagatctct
     5461 ttcaccatca cagagcttga caactctaaa tgatccaaac tctccagaaa gacgacggcg
     5521 aaaaatgggt tcaactcgca agaaccctaa acggcagttc aaagcagaaa gagaagatga
     5581 agacaaagta gatcagaaga cagagtcaaa caaagaagaa ataacggagg ggtcgctagt
     5641 gcataatatc acagaagacc ctccaaataa agagtatctt gatgttccca gtgcacacac
     5701 aagcagccag caagaagaga gcgatagccc tgttgttcaa gaaagtgcgt caccatcaac
     5761 taaaaggaag tttgggtcca gacgagctaa taagggcaaa cttggtaggc ttgaggcatc
     5821 tgatggtatg actgaatcag aagatggaga tcataaacca gatggctctg ctgttgagga
     5881 cagacagcac agggatggtt caatgggttg tggtacgagc gtcagcttac aaccagaaaa
     5941 tctgccattt tccagaaatt atgaagttgc tccaaaaggc actggtgaag agattcaagt
     6001 cagaaaacag gacagaatga cggtcaatgt aaagaacagg gattctaaag tggttcagtt
     6061 taatgtggtc atggtgggaa acagctgtgt tggaaagacg tccttcataa gacgtttcca
     6121 tgaaggccag ttcaccgagg attaccgctc cactattggt gttgacactt tcgtacagac
     6181 tgttgagctg cccgacagaa ctgttaaact gcagatatgg gacacagcag gtcaagagag
     6241 gtttcacagc atcaccacgc aagttttcca caaggctgag gggcttctgc tcatgtatga
     6301 gatcacatgt tcaaagagtt tcatttctat acgagattgg atctctcaag cccgggaaag
     6361 ggctcaagat gatgtcgtca tgatgttgct cggaaacaag aatgattcag ttaatcgtga
     6421 agtccagatt caggaaggag cagacctggc cagagagtat aacatacatt tcatggagtg
     6481 cagtgctgca aacggggcta atgtgtcaga atctatgagg actcttgctg agttgttagt
     6541 gcagcgcaag agaaaaaggg aggaacacac aacgctgaga agagaaccac aacagaagaa
     6601 atctggatgc tgttaaccgc ggcaacttta ttatacatag ttgataattc actggccgtc
     6661 gttttacggt accatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     6721 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6781 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6841 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6901 atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc
     6961 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
     7021 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
     7081 tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct
     7141 gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta
     7201 aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg
     7261 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
     7321 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
     7381 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
     7441 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
     7501 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     7561 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7621 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7681 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7741 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7801 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7861 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7921 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8401 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg
     8641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8701 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8761 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8821 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8881 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8941 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9001 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9061 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9121 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9181 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9241 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9301 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9361 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9421 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9481 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9541 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9601 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9661 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9721 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     9781 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     9841 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     9901 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccacgctggc
     9961 taagaactca tcagcctccc cggtccatct acccacgtac caatgcacca attggccaca
    10021 atgacggcta ctacatggtg ccattccttc ctctttatag gaatggagac tacctcctgt
    10081 ccaacaatgc tcttggatac gagtacgcct acctgttgga cccaggtcat tgcacaacac
    10141 cagaaatgcc ctctgatctg caaaagacgt gaatatctgt tcagacaccc atatccactc
    10201 tgttccacac aggtcagagg tttgtccagg agttcttgac agaggtgtaa aaagtactca
    10261 aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa
    10321 aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac
    10381 ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa ctagagattc ttgtttaagc
    10441 ttttaatctc aaaaaacatt aaatgaaatg catacaaggt tttatcctgc tttagaactg
    10501 tttgtattta attatcaaac tataagacag acaatctaat gccagtacac gctactcaaa
    10561 gttgtaaaac ctcagattta acttcagtag aagctgattc tcaaaattgt tagtgtcaag
    10621 cctagctctt ttggggctga aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc
    10681 cgatgcggga agaggtgtat tagtcttgat agagaggctg caaatagcag gaaacgtgag
    10741 cagagactcc ctggtgtctg aaacacaggc cagatgggcc ctcgagcagg aaacagctat
    10801 gaccatgatt acgccaagct atcaactttg tatagaaaag ttggcacgcg cagattccgt
    10861 gtgggcctgg taatatctta ccattttaag ctattttaag attagggaaa aaaattaaat
    10921 aaaactactt taaaattact ttccttaaaa agcaacatat ttatttagtt tttgtttaac
    10981 tggctcaaga ttgtaatatt ttacttttac atgttttttt aagtacctaa atgtgttcaa
    11041 aagtaaagta tgttattgtt tttttttttt aatgttagct aagctttaaa ttgtaatata
    11101 ttacttttaa aagtgacttt ttaaaaatgt aaataaaaaa gtaactataa atgtgtaatg
    11161 catgttattt ctgtgggttt ttaattttca tctcaactgg gcttacaatt ttaacataat
    11221 aaaaatcaaa tcaaatcaaa tcaggccctt atttgtcaca tacactcttg tatactgaaa
    11281 taaactaaat gttgagaaaa atttaaatac accaaaataa cgcatacctt ttttgtaatt
    11341 acctaagtaa aacatttagt tcctttttta agtgactcaa gattgtaatc tattaattgt
    11401 aaaagataaa actagtgatc aatctgacgt gcttctatgc agcctgtgag attaattgtg
    11461 atgtttaatt attaaacaat ttaaatggat caaacatttc acatgtgctt attgtgtcca
    11521 acaaaaccag cagaagcact taaaaatatt gcatgacaac atgagttttt aatgtaaatt
    11581 atatatactt cctaaacgaa gttaatcata tttctaaaat aataatgtca ttgaaatatt
    11641 taaataaatg ataagcttta gttgattcaa cataaaattg aaagcaataa ggactttttt
    11701 tttttacagc ggtgaagcaa aagcatgtaa taatcatata atataataaa taaataaata
    11761 aataatggct cttggtgcaa atatgaacac tttaaataaa tatagaataa atatgatcaa
    11821 taagtcataa cgatgtgttt tatgttgatt ataatttaaa catgtttcta acgcaagttt
    11881 taaaaactgt tttaaagtat tattttaagt tataagatca atttgactca acattttgta
    11941 gtgaaaatat gttgtgtaaa attaatataa tatacactat aaaaatgcag ggttccacac
    12001 aatacattaa tgttgtccca acacaaatcg attaagttaa caaatatatg tggattgaac
    12061 ataaaaaatt aagttgtccc aatgaaatct caagaattgt gttgtttcag ctcattttaa
    12121 ataagtagtt ttaacaagta aaaaataaca tatttttgag tgtgtatata tatatattac
    12181 aaaatataat aatataaaat gtaaaagata taaaccatgc tacatatatt taagagctat
    12241 tttgcacatt acttttttgc aagccatttt agtgtttttt ttacaaatat atattagcta
    12301 aatatacaat atcttaaata tacaattaat cagacatgca attaatgcat ttaacctaca
    12361 taaaaataca tgatcaacat atgttttatg ctgattacaa ctaaatcaaa cgtaatgaaa
    12421 ttaatttata aaagcgattt aaatatgtca atattttaag ttaaaatcac ttgaaaccta
    12481 ctataaatga acttaaatta aaaaaatctc ctttaattcc aatgcaacag tgttgttttt
    12541 tcagctgagg acccccggtg cagatggttg gcccccggcc cgctattcta aggaagcctt
    12601 tcttaatttt agggcggcgc tgcatgcgct cagatcagat tcacgggtgg agagggttat
    12661 tttctctttt ttattttttc ttgcctgctc atatttgggc ctgtgtgaac cccaggctcc
    12721 gcctcggccc ccaggagccg ctcttattta aaggggatcc tcgccttcag tcttcgtccc
    12781 agtggaagca tcagtcttgt gataacaact tggtgagtcc tacagaccag cgctcgactc
    12841 taggctttaa atgcattgat ttctctggat tagctaccgg gagagtttta actttgctta
    12901 ttagtcttgt gctcgaactg ctaggtttaa agagtgtttt ctctaatgca tgcatgaagc
    12961 gacagctgaa aaccagaaat agagcgtttg tcctcctgtt aaactctagc ctttctttgg
    13021 catttgtcag tccgagtctt cagctgccgg caggagattg ggtttcagta acacttgctg
    13081 agcacaaact acacgtgctt tcttcagatt taattcatta ttaatcattc tagatttaaa
    13141 tgatgttctt actcattgct gattatttta gccttgcgga gcagattaaa tgagcacgtg
    13201 tttcattaag attgatttaa atataattaa tatttcacca ctgtagtttt aaaattgcct
    13261 tctaagtcat ggttgatttt taataggtat cggctctaat tagattacta ttttggtttt
    13321 taaatgttat ttctatttca tttgtaggct cgtttttaat gtgattttat tagattctag
    13381 ttttaaaatc atctcttagc cttaatgagc agactgaatg aacatgtttc attaagatta
    13441 acttcattat aatgaattat ttaatcattc tagtgggtta taaatcagct ctatttaaat
    13501 taattgtcgt gtttatttct atttaatttt tgattatttt ttcagggtcg tttttatgtg
    13561 attttttccc gtttctaatt ttaaactaat tgtttgattt ttatatgatt cgactctttt
    13621 taaatgaatt gttttgcttt ttttattgag catatatata ttatacaatt tcaatcaaca
    13681 attgttgtgc tttttaaatg ttgtttctat ttcatttcta gggtcgattt tattcgattc
    13741 tagttttaaa atcgtgattt ttaacttcta accttactga gcagactgaa tgaacatgtt
    13801 tcatcaagat taacttcatt atagttaatt attgaatcgt gctagtggtt tataaatcag
    13861 ctctatttaa attaatcatc atcattattt ctatttaatt tttattattt ctttaggatt
    13921 atttttatgt gatttttttc acgttctagt tttaaactca atgctcatta ctgctcaccc
    13981 ttgctgagca gtctttcgtt aagattaaat taattaataa ccattctagt tttaaaacag
    14041 tgtcttaagg catagctgat attttacaag tttactattt aatatatctc ctttcttttt
    14101 ttagggtcga tttcatctga tttgattaat ttttattatc ttcactaagc tttttgttct
    14161 aggttttatt ctcagtatta ttactgttat atttgaactg aatttctcct catagtttat
    14221 tcattttatt tatttatttt ttgtttattc gctattttgt attttagttt ttaattagaa
    14281 attttattag aatcgttttg aactagtttt tatgcactct aacattaata ttaaatcata
    14341 actattattt ttattataat ttatttgtgt ttatcttttt gtgtagcttt catgtgtttt
    14401 tattttagtt ttattatgtt tgcattgaat tttttggact agtttatata acgatataca
    14461 gaaaagccat gacaacaaat gtttttataa tacctattaa gtataagcta atcagatttc
    14521 cactattctg ttcaaagtta acattattag ccattctgat ggatcatttg acatttgtca
    14581 tatgtcttta tgaacactgc tctttcagat gccactttta aagctgtttt ttacagtaaa
    14641 tgaacgttta ttattatgac attatttcct aaggcctcta gagtacagca ggatgtggcg
    14701 tgtgcattac cgtgtaatta tgctgactgt aaatgtgcat ttatagactg tcagcatgtc
    14761 tgactgtgcg ctgttgtgat tggtcctgca cagaccaag
//
