LOCUS       sequence-224798-       11819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to alpha 
            actinin 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 11819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-actn2-mKate2
FEATURES             Location/Qualifiers
     source          1..11819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2718..2742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2744..3439
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    3561..3642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3643..3663)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3671..3687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3703..3837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3727..3746)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(4276..4463,4464..4475)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4509..4525)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4509..4525)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4562..4582)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4562..4580)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4601..4617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4614..4636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4625..4641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4649..4655,4656..4673,4674..4679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4694..4715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4832..4849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5003..5591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5083..5102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5762..6553,6554..6622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6385..6404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6623..6727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6890..6911)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(7260..7271,7272..7434)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     7807..7823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7843..7863
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catgatgaat cagatcgagc tttcagtgcc
       61 ttatgataac ggatatgaaa tagacgagta catgatgcag gaggatgaat gggaccggga
      121 tttactgctg gatccagcat gggaaaagca acaaaggaag acgttcactg cctggtgtaa
      181 ctctcacctg agaaaagctg ggacgcagat cgagaacata gaggaggact ttagaaatgg
      241 cctcaaacta atgctgctgc ttgaagtcat ctcaggtgag aggttaccca agcctgacag
      301 agggaagatg cgttttcaca agatagctaa tgtcaataaa gccctggatt tcatcaccag
      361 taaaggtgtg aaactggtct ccattggagc tgaagaaatt gtggatggta atgtgaaaat
      421 gactcttgga atgatctgga caatcatcct tcgcttcgcc attcaggata tctctgttga
      481 agaaacttcg gccaaagaag gacttctgct gtggtgtcag agaaagactg ccccgtaccg
      541 aaatgtcaat gttcaaaact tccatgtcag ctggaaggat ggattggcgc tgtgcgctct
      601 catccacagg cacaggcctg acctcattga ctacgccaag ctcaacaagg atgatccact
      661 gggtaattta aatctggcat ttgacattgc tgaaaaacat ctggacatcc ccaaaatgct
      721 ggatgcagag gacatcctga gcacccctaa acctgatgag agggctatta tgacatacgt
      781 gtcctgcttt taccatgcat ttgctggagc tgagcaggct gaaactgctg cgaacaggat
      841 ctgtaaggtg cttggagtga atcaggaaaa tgagaagttg atggaggatt atgaaaggct
      901 ggctagtgag ctgttggagt ggatcagacg taccattcct tggctggaga atcgtgcagc
      961 tgagaagacc atggttgaga tgcagaggaa gctggaagat tttcgagatt accgcaggat
     1021 gcacaaacca cccaaagtgc aggagaaatg tcagctggaa atcagcttca acacgctgca
     1081 gaccaaactc cgcatcagca accggcctgc gtttatgccc tctgagggca aaatggtgtc
     1141 ggacatagcg agtgcgtggc aagggctgga acaagcagag aagggctatg aggaatggct
     1201 gctgactgag atcaggaggc tggaaagagt ggagcatcta gctgagaaat tcagacagaa
     1261 agctactaac catgagtcct gggctacagg taaggaagag atgttgaccc ggaaggacta
     1321 cgagtcagcg tctctaatgg aagtgcgagc tttgctgcgg aaacacgaag cgtttgagag
     1381 tgacctttct gcacaccagg acagagttga gcagatcgct gctattgctc aggagctcaa
     1441 tgagctggac tatcatgatg ttgcatccgt gaatcaaagg tgtcaaagta tttgtgacct
     1501 ctgggaccat ctgggcacgc tcacccagaa acgcagagag gctttggaga ggacagagaa
     1561 acttttggag acggtggatc agttgtttct ggaatatgca aagagatctg caccctttaa
     1621 taactggatg gaaggagcta tggaagactt gcaggacatg ttcattgtgc acacaattga
     1681 ggaagtccag actctgatag ctgctcatga gcagtttaag gccacccttc ctgaggcaga
     1741 tgcagagaga caggcgatcc ttggcattca gcaggaagtc ctgaagatct tccagagtta
     1801 tggcatccgt ggagatctta caaatcccta cagcactatt actgcagaag aaattgccat
     1861 caaatgggac aaggtgaaga agctggtgcc ccagcgtgac agtgcactgc aggaggagtt
     1921 agcgagacag catgctaacg aaagactcag acgccagttt gctgcccaag ccaacctcat
     1981 cggaccctgg atacagacta ggatggagga aattggtcat tgttctatgg cgatgggcgg
     2041 aactctggag gatcagatga cccaactgaa gcagtatgag catgtgatcg tcagctacaa
     2101 atccaacata gaccgacttg agggagatca ccaactcatt caggagtcac ttatctttga
     2161 caacaaacac actaactaca ctatggagca tgctcgcgtt ggctgggagc tcctattgac
     2221 cacgattgct cgcacaatta atgaaatcga gactcaaatt ttgacccgtg atgcaaaagg
     2281 gatcagccag gagcagatgc atgagttccg gtcctctttc aaccattttg atcggaagaa
     2341 gaacgggggc atggattctg atgactttag ggcctgtctg atctcgatgg gttatgatct
     2401 gggagaggtg gagtttgctc ggatcatgat gctggtggat ccaaatgctt caggcaatgt
     2461 gtcgttccag tccttcattg atttcatgac tagagagaca gcagacacag acacagctga
     2521 acaggtcatt gcttccttca agatcctcgc tgcagacaag ccgtatattc tgtcagatga
     2581 gttgaggcga gagcttcctc cagaccaggc cgagtactgt atcagtcgaa tgcctcctta
     2641 ctgtggcccc ggggccctgc ctggagcctt ggactacacc gccttctcca ccgccctata
     2701 tggagaaagt gaccttgacc cagctttctt gtacaaagtg gctgtgagcg agctgattaa
     2761 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg
     2821 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt
     2881 cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag
     2941 caaaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga
     3001 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     3061 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     3121 cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac
     3181 cctgtacccc gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg
     3241 cgggggccac ctgatctgca acttgaagac cacatacaga tccaagaaac ccgctaagaa
     3301 cctcaagatg cccggcgtct actatgtgga cagaagactg gaaagaatca aggaggccga
     3361 caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
     3421 caaactgggg cacagatgag cggccgcgac tctagatcat aatcagccat accacatttg
     3481 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3541 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3601 atagcatcac aaatttcaca aataaagcat ttttttcact gccaacttta ttatacatag
     3661 ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga catgataaga
     3721 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3781 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3841 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     3901 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc
     3961 gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag
     4021 ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt ataatttccc
     4081 taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca
     4141 aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag
     4201 aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca
     4261 agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt
     4321 gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat
     4381 tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta
     4441 atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa
     4501 gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
     4561 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4801 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5401 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5641 actcacgtta aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6061 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6121 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6181 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6241 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6301 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6361 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6421 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6481 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6541 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6601 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6661 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6721 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     6781 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     6841 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     6901 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     6961 gggcgatggc ccacgctggc taagaactca tcagcctccc cggtccatct acccacgtac
     7021 caatgcacca attggccaca atgacggcta ctacatggtg ccattccttc ctctttatag
     7081 gaatggagac tacctcctgt ccaacaatgc tcttggatac gagtacgcct acctgttgga
     7141 cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt gaatatctgt
     7201 tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg agttcttgac
     7261 agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa
     7321 ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa
     7381 gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa
     7441 ctagagattc ttgtttaagc ttttaatctc aaaaaacatt aaatgaaatg catacaaggt
     7501 tttatcctgc tttagaactg tttgtattta attatcaaac tataagacag acaatctaat
     7561 gccagtacac gctactcaaa gttgtaaaac ctcagattta acttcagtag aagctgattc
     7621 tcaaaattgt tagtgtcaag cctagctctt ttggggctga aaagcaatcc tgcagtgctg
     7681 aaaagcctct cacaggcagc cgatgcggga agaggtgtat tagtcttgat agagaggctg
     7741 caaatagcag gaaacgtgag cagagactcc ctggtgtctg aaacacaggc cagatgggcc
     7801 ctcgagcagg aaacagctat gaccatgatt acgccaagct atcaactttg tatagaaaag
     7861 ttggcacgcg cagattccgt gtgggcctgg taatatctta ccattttaag ctattttaag
     7921 attagggaaa aaaattaaat aaaactactt taaaattact ttccttaaaa agcaacatat
     7981 ttatttagtt tttgtttaac tggctcaaga ttgtaatatt ttacttttac atgttttttt
     8041 aagtacctaa atgtgttcaa aagtaaagta tgttattgtt tttttttttt aatgttagct
     8101 aagctttaaa ttgtaatata ttacttttaa aagtgacttt ttaaaaatgt aaataaaaaa
     8161 gtaactataa atgtgtaatg catgttattt ctgtgggttt ttaattttca tctcaactgg
     8221 gcttacaatt ttaacataat aaaaatcaaa tcaaatcaaa tcaggccctt atttgtcaca
     8281 tacactcttg tatactgaaa taaactaaat gttgagaaaa atttaaatac accaaaataa
     8341 cgcatacctt ttttgtaatt acctaagtaa aacatttagt tcctttttta agtgactcaa
     8401 gattgtaatc tattaattgt aaaagataaa actagtgatc aatctgacgt gcttctatgc
     8461 agcctgtgag attaattgtg atgtttaatt attaaacaat ttaaatggat caaacatttc
     8521 acatgtgctt attgtgtcca acaaaaccag cagaagcact taaaaatatt gcatgacaac
     8581 atgagttttt aatgtaaatt atatatactt cctaaacgaa gttaatcata tttctaaaat
     8641 aataatgtca ttgaaatatt taaataaatg ataagcttta gttgattcaa cataaaattg
     8701 aaagcaataa ggactttttt tttttacagc ggtgaagcaa aagcatgtaa taatcatata
     8761 atataataaa taaataaata aataatggct cttggtgcaa atatgaacac tttaaataaa
     8821 tatagaataa atatgatcaa taagtcataa cgatgtgttt tatgttgatt ataatttaaa
     8881 catgtttcta acgcaagttt taaaaactgt tttaaagtat tattttaagt tataagatca
     8941 atttgactca acattttgta gtgaaaatat gttgtgtaaa attaatataa tatacactat
     9001 aaaaatgcag ggttccacac aatacattaa tgttgtccca acacaaatcg attaagttaa
     9061 caaatatatg tggattgaac ataaaaaatt aagttgtccc aatgaaatct caagaattgt
     9121 gttgtttcag ctcattttaa ataagtagtt ttaacaagta aaaaataaca tatttttgag
     9181 tgtgtatata tatatattac aaaatataat aatataaaat gtaaaagata taaaccatgc
     9241 tacatatatt taagagctat tttgcacatt acttttttgc aagccatttt agtgtttttt
     9301 ttacaaatat atattagcta aatatacaat atcttaaata tacaattaat cagacatgca
     9361 attaatgcat ttaacctaca taaaaataca tgatcaacat atgttttatg ctgattacaa
     9421 ctaaatcaaa cgtaatgaaa ttaatttata aaagcgattt aaatatgtca atattttaag
     9481 ttaaaatcac ttgaaaccta ctataaatga acttaaatta aaaaaatctc ctttaattcc
     9541 aatgcaacag tgttgttttt tcagctgagg acccccggtg cagatggttg gcccccggcc
     9601 cgctattcta aggaagcctt tcttaatttt agggcggcgc tgcatgcgct cagatcagat
     9661 tcacgggtgg agagggttat tttctctttt ttattttttc ttgcctgctc atatttgggc
     9721 ctgtgtgaac cccaggctcc gcctcggccc ccaggagccg ctcttattta aaggggatcc
     9781 tcgccttcag tcttcgtccc agtggaagca tcagtcttgt gataacaact tggtgagtcc
     9841 tacagaccag cgctcgactc taggctttaa atgcattgat ttctctggat tagctaccgg
     9901 gagagtttta actttgctta ttagtcttgt gctcgaactg ctaggtttaa agagtgtttt
     9961 ctctaatgca tgcatgaagc gacagctgaa aaccagaaat agagcgtttg tcctcctgtt
    10021 aaactctagc ctttctttgg catttgtcag tccgagtctt cagctgccgg caggagattg
    10081 ggtttcagta acacttgctg agcacaaact acacgtgctt tcttcagatt taattcatta
    10141 ttaatcattc tagatttaaa tgatgttctt actcattgct gattatttta gccttgcgga
    10201 gcagattaaa tgagcacgtg tttcattaag attgatttaa atataattaa tatttcacca
    10261 ctgtagtttt aaaattgcct tctaagtcat ggttgatttt taataggtat cggctctaat
    10321 tagattacta ttttggtttt taaatgttat ttctatttca tttgtaggct cgtttttaat
    10381 gtgattttat tagattctag ttttaaaatc atctcttagc cttaatgagc agactgaatg
    10441 aacatgtttc attaagatta acttcattat aatgaattat ttaatcattc tagtgggtta
    10501 taaatcagct ctatttaaat taattgtcgt gtttatttct atttaatttt tgattatttt
    10561 ttcagggtcg tttttatgtg attttttccc gtttctaatt ttaaactaat tgtttgattt
    10621 ttatatgatt cgactctttt taaatgaatt gttttgcttt ttttattgag catatatata
    10681 ttatacaatt tcaatcaaca attgttgtgc tttttaaatg ttgtttctat ttcatttcta
    10741 gggtcgattt tattcgattc tagttttaaa atcgtgattt ttaacttcta accttactga
    10801 gcagactgaa tgaacatgtt tcatcaagat taacttcatt atagttaatt attgaatcgt
    10861 gctagtggtt tataaatcag ctctatttaa attaatcatc atcattattt ctatttaatt
    10921 tttattattt ctttaggatt atttttatgt gatttttttc acgttctagt tttaaactca
    10981 atgctcatta ctgctcaccc ttgctgagca gtctttcgtt aagattaaat taattaataa
    11041 ccattctagt tttaaaacag tgtcttaagg catagctgat attttacaag tttactattt
    11101 aatatatctc ctttcttttt ttagggtcga tttcatctga tttgattaat ttttattatc
    11161 ttcactaagc tttttgttct aggttttatt ctcagtatta ttactgttat atttgaactg
    11221 aatttctcct catagtttat tcattttatt tatttatttt ttgtttattc gctattttgt
    11281 attttagttt ttaattagaa attttattag aatcgttttg aactagtttt tatgcactct
    11341 aacattaata ttaaatcata actattattt ttattataat ttatttgtgt ttatcttttt
    11401 gtgtagcttt catgtgtttt tattttagtt ttattatgtt tgcattgaat tttttggact
    11461 agtttatata acgatataca gaaaagccat gacaacaaat gtttttataa tacctattaa
    11521 gtataagcta atcagatttc cactattctg ttcaaagtta acattattag ccattctgat
    11581 ggatcatttg acatttgtca tatgtcttta tgaacactgc tctttcagat gccactttta
    11641 aagctgtttt ttacagtaaa tgaacgttta ttattatgac attatttcct aaggcctcta
    11701 gagtacagca ggatgtggcg tgtgcattac cgtgtaatta tgctgactgt aaatgtgcat
    11761 ttatagactg tcagcatgtc tgactgtgcg ctgttgtgat tggtcctgca cagaccaag
//
