LOCUS       sequence-224799-        9581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to Rab3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-Rab3b
FEATURES             Location/Qualifiers
     source          1..9581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1406..1426)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1434..1450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1466..1600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1490..1509)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1544..1563
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2039..2226,2227..2238)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2272..2288)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2288)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2325..2345)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2325..2343)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2364..2380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2377..2399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2388..2404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2412..2418,2419..2436,2437..2442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2457..2478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2595..2612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2766..3354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2846..2865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3525..4316,4317..4385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4148..4167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4386..4490)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4653..4674)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5023..5034,5035..5197)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5570..5586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5606..5626
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcaaaa gcagaccagc gctttggtca
      781 gagagatgga tctgatcaaa acttcgacta catgttcaaa ctcctgatca tcggcaacag
      841 cagcgtagga aaaaccagct tcttattccg atacgcagac gactccttca gcagctcgtt
      901 tgtcagcaca gtcggcatcg acttcaaggt gaagacggtg tacagaaacg acaagcgagt
      961 gaagctccag atatgggata cggcgggaca ggagcggtat agaactatta ccacagctta
     1021 ctatagagga gccatgggct tcatcctcat gtatgacatc actaatgagg agtcctataa
     1081 tgcggtgcag gactgggcaa cacaaattaa gacttattca tgggataacg ctcaggtgat
     1141 actagtggga aacaaatgtg acatggatga ggagagggtg gtgccttttg agaagggaaa
     1201 acacttggct gaccagttag ggtttgagta ctatgaagcc agcgccaaag agaacatcaa
     1261 cgtcagacaa gtatttgaac gcttagtcga cattatctgc gtgaagatgt cagagagagt
     1321 tgatgtggaa ccacccatgg tcactggtgc caaaaccacc cggctaaccg ataagccacc
     1381 ccaactacct cagaattgtt gctgacaact ttattataca tagttgataa ttcactggcc
     1441 gtcgttttac ggtaccatcg atgatgatcc agacatgata agatacattg atgagtttgg
     1501 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1561 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1621 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     1681 caaatgtggt atggctgatt atgatcctct agatcagatc tgcgaagata cggccacggg
     1741 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
     1801 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
     1861 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
     1921 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
     1981 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
     2041 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     2101 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     2161 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     2221 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
     2281 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     2341 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2401 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2461 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2521 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2581 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2641 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3181 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3361 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaaggcatt
     3421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3481 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3541 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3601 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3661 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3721 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3781 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3841 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3901 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3961 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4021 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4081 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4141 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4201 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4261 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4321 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4381 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4441 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4501 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4561 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4621 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4681 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccacgct
     4741 ggctaagaac tcatcagcct ccccggtcca tctacccacg taccaatgca ccaattggcc
     4801 acaatgacgg ctactacatg gtgccattcc ttcctcttta taggaatgga gactacctcc
     4861 tgtccaacaa tgctcttgga tacgagtacg cctacctgtt ggacccaggt cattgcacaa
     4921 caccagaaat gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca
     4981 ctctgttcca cacaggtcag aggtttgtcc aggagttctt gacagaggtg taaaaagtac
     5041 tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag
     5101 taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg
     5161 tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta
     5221 agcttttaat ctcaaaaaac attaaatgaa atgcatacaa ggttttatcc tgctttagaa
     5281 ctgtttgtat ttaattatca aactataaga cagacaatct aatgccagta cacgctactc
     5341 aaagttgtaa aacctcagat ttaacttcag tagaagctga ttctcaaaat tgttagtgtc
     5401 aagcctagct cttttggggc tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc
     5461 agccgatgcg ggaagaggtg tattagtctt gatagagagg ctgcaaatag caggaaacgt
     5521 gagcagagac tccctggtgt ctgaaacaca ggccagatgg gccctcgagc aggaaacagc
     5581 tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttggcac gcgcagattc
     5641 cgtgtgggcc tggtaatatc ttaccatttt aagctatttt aagattaggg aaaaaaatta
     5701 aataaaacta ctttaaaatt actttcctta aaaagcaaca tatttattta gtttttgttt
     5761 aactggctca agattgtaat attttacttt tacatgtttt tttaagtacc taaatgtgtt
     5821 caaaagtaaa gtatgttatt gttttttttt tttaatgtta gctaagcttt aaattgtaat
     5881 atattacttt taaaagtgac tttttaaaaa tgtaaataaa aaagtaacta taaatgtgta
     5941 atgcatgtta tttctgtggg tttttaattt tcatctcaac tgggcttaca attttaacat
     6001 aataaaaatc aaatcaaatc aaatcaggcc cttatttgtc acatacactc ttgtatactg
     6061 aaataaacta aatgttgaga aaaatttaaa tacaccaaaa taacgcatac cttttttgta
     6121 attacctaag taaaacattt agttcctttt ttaagtgact caagattgta atctattaat
     6181 tgtaaaagat aaaactagtg atcaatctga cgtgcttcta tgcagcctgt gagattaatt
     6241 gtgatgttta attattaaac aatttaaatg gatcaaacat ttcacatgtg cttattgtgt
     6301 ccaacaaaac cagcagaagc acttaaaaat attgcatgac aacatgagtt tttaatgtaa
     6361 attatatata cttcctaaac gaagttaatc atatttctaa aataataatg tcattgaaat
     6421 atttaaataa atgataagct ttagttgatt caacataaaa ttgaaagcaa taaggacttt
     6481 tttttttaca gcggtgaagc aaaagcatgt aataatcata taatataata aataaataaa
     6541 taaataatgg ctcttggtgc aaatatgaac actttaaata aatatagaat aaatatgatc
     6601 aataagtcat aacgatgtgt tttatgttga ttataattta aacatgtttc taacgcaagt
     6661 tttaaaaact gttttaaagt attattttaa gttataagat caatttgact caacattttg
     6721 tagtgaaaat atgttgtgta aaattaatat aatatacact ataaaaatgc agggttccac
     6781 acaatacatt aatgttgtcc caacacaaat cgattaagtt aacaaatata tgtggattga
     6841 acataaaaaa ttaagttgtc ccaatgaaat ctcaagaatt gtgttgtttc agctcatttt
     6901 aaataagtag ttttaacaag taaaaaataa catatttttg agtgtgtata tatatatatt
     6961 acaaaatata ataatataaa atgtaaaaga tataaaccat gctacatata tttaagagct
     7021 attttgcaca ttactttttt gcaagccatt ttagtgtttt ttttacaaat atatattagc
     7081 taaatataca atatcttaaa tatacaatta atcagacatg caattaatgc atttaaccta
     7141 cataaaaata catgatcaac atatgtttta tgctgattac aactaaatca aacgtaatga
     7201 aattaattta taaaagcgat ttaaatatgt caatatttta agttaaaatc acttgaaacc
     7261 tactataaat gaacttaaat taaaaaaatc tcctttaatt ccaatgcaac agtgttgttt
     7321 tttcagctga ggacccccgg tgcagatggt tggcccccgg cccgctattc taaggaagcc
     7381 tttcttaatt ttagggcggc gctgcatgcg ctcagatcag attcacgggt ggagagggtt
     7441 attttctctt ttttattttt tcttgcctgc tcatatttgg gcctgtgtga accccaggct
     7501 ccgcctcggc ccccaggagc cgctcttatt taaaggggat cctcgccttc agtcttcgtc
     7561 ccagtggaag catcagtctt gtgataacaa cttggtgagt cctacagacc agcgctcgac
     7621 tctaggcttt aaatgcattg atttctctgg attagctacc gggagagttt taactttgct
     7681 tattagtctt gtgctcgaac tgctaggttt aaagagtgtt ttctctaatg catgcatgaa
     7741 gcgacagctg aaaaccagaa atagagcgtt tgtcctcctg ttaaactcta gcctttcttt
     7801 ggcatttgtc agtccgagtc ttcagctgcc ggcaggagat tgggtttcag taacacttgc
     7861 tgagcacaaa ctacacgtgc tttcttcaga tttaattcat tattaatcat tctagattta
     7921 aatgatgttc ttactcattg ctgattattt tagccttgcg gagcagatta aatgagcacg
     7981 tgtttcatta agattgattt aaatataatt aatatttcac cactgtagtt ttaaaattgc
     8041 cttctaagtc atggttgatt tttaataggt atcggctcta attagattac tattttggtt
     8101 tttaaatgtt atttctattt catttgtagg ctcgttttta atgtgatttt attagattct
     8161 agttttaaaa tcatctctta gccttaatga gcagactgaa tgaacatgtt tcattaagat
     8221 taacttcatt ataatgaatt atttaatcat tctagtgggt tataaatcag ctctatttaa
     8281 attaattgtc gtgtttattt ctatttaatt tttgattatt ttttcagggt cgtttttatg
     8341 tgattttttc ccgtttctaa ttttaaacta attgtttgat ttttatatga ttcgactctt
     8401 tttaaatgaa ttgttttgct ttttttattg agcatatata tattatacaa tttcaatcaa
     8461 caattgttgt gctttttaaa tgttgtttct atttcatttc tagggtcgat tttattcgat
     8521 tctagtttta aaatcgtgat ttttaacttc taaccttact gagcagactg aatgaacatg
     8581 tttcatcaag attaacttca ttatagttaa ttattgaatc gtgctagtgg tttataaatc
     8641 agctctattt aaattaatca tcatcattat ttctatttaa tttttattat ttctttagga
     8701 ttatttttat gtgatttttt tcacgttcta gttttaaact caatgctcat tactgctcac
     8761 ccttgctgag cagtctttcg ttaagattaa attaattaat aaccattcta gttttaaaac
     8821 agtgtcttaa ggcatagctg atattttaca agtttactat ttaatatatc tcctttcttt
     8881 ttttagggtc gatttcatct gatttgatta atttttatta tcttcactaa gctttttgtt
     8941 ctaggtttta ttctcagtat tattactgtt atatttgaac tgaatttctc ctcatagttt
     9001 attcatttta tttatttatt ttttgtttat tcgctatttt gtattttagt ttttaattag
     9061 aaattttatt agaatcgttt tgaactagtt tttatgcact ctaacattaa tattaaatca
     9121 taactattat ttttattata atttatttgt gtttatcttt ttgtgtagct ttcatgtgtt
     9181 tttattttag ttttattatg tttgcattga attttttgga ctagtttata taacgatata
     9241 cagaaaagcc atgacaacaa atgtttttat aatacctatt aagtataagc taatcagatt
     9301 tccactattc tgttcaaagt taacattatt agccattctg atggatcatt tgacatttgt
     9361 catatgtctt tatgaacact gctctttcag atgccacttt taaagctgtt ttttacagta
     9421 aatgaacgtt tattattatg acattatttc ctaaggcctc tagagtacag caggatgtgg
     9481 cgtgtgcatt accgtgtaat tatgctgact gtaaatgtgc atttatagac tgtcagcatg
     9541 tctgactgtg cgctgttgtg attggtcctg cacagaccaa g
//
