LOCUS       sequence-224843-       10560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to EHD2b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-EHD2b
FEATURES             Location/Qualifiers
     source          1..10560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2384..2404)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2412..2428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2444..2578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2468..2487)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2522..2541
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3017..3204,3205..3216)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3250..3266)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3250..3266)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3303..3323)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3303..3321)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3342..3358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3355..3377)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3366..3382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3390..3396,3397..3414,3415..3420))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3435..3456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3573..3590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3744..4332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3824..3843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4503..5294,5295..5363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5126..5145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5364..5468)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5631..5652)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6001..6012,6013..6175)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6548..6564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6584..6604
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgtcacgc tggggacgta agaatgtcaa
      781 gaaggccccc gaggtgatca ggacagtgac cgagggcctg aagtcactct accgaaaaaa
      841 gctcctgcct ctggagcagt attacggctt ccatgacttc cactcaccga gtctggagga
      901 cgccgacttt gacaacaaac ccatggtgct ggtcgttggg cagtactcca cgggaaagac
      961 caccttcatc aagtatctgc ttgaacagga tgtccctggg agccgcatag ggcctgagcc
     1021 caccaccgac tgcttcaccg ctatcatgca cggagacgta gaggggctca ttcccggaaa
     1081 tgccctcatc gtggatccca acaagccctt ccgcaaactc aaccccttcg gaaacacctt
     1141 tctcaacaga ttccagtgcg ctcagctgcc caaccaggtc ctggagagca tcagcattat
     1201 tgacacccca ggaatcctgt ctggggccaa acagagagtg agtcgaggat atgatttccc
     1261 agcagttctc cgctggtttg ctgagcgtgt ggaccgaatc atccttctct ttgatgccca
     1321 caagttagag atctcagatg aattctcaga ggctatcgga gctctcaaag gcaatgaaga
     1381 caaactccgt gttgtgctga acaaggctga tatggttgat acccagcagc ttatgcgggt
     1441 gtacggagct ttaatgtggt ccctggggaa agtgtttggt actcctgagg ttctgagagt
     1501 gtacatcggt tccttctggt ctgaacctct catggtgacc gacaaccgca agctgttcga
     1561 attggaggag gaagatctgt ttgctgatat ccagaatctg ccacgtaatg cagccctacg
     1621 caaacttaat gacttggtca agagggcccg tcttgtgagg gtacatgccc atattatcag
     1681 ttacttgaaa caagagatgc catcggtctt cagaaaagac aataaaaaga agaacctgat
     1741 ctaccagctc ccggtcatct tctccaagat ccaactacaa catcacatct ctcctggaga
     1801 cttccctgac tgtgccaaga tgcaggagca actaatggca catgacttca ccaagtttaa
     1861 agccctgaag cccaacctta tgaatatgct ggatgagctg ttgtccagtg acattgccaa
     1921 actgatgccc cttcttaggc aggaagaaat agaggctggg gttcaaccag gtgtccaagg
     1981 tggagctttc ttgggaaccc gtgccggacc ctttgtggaa ggtgacccct ttggagaggt
     2041 tgcgaatgaa aatggcgaag tgggtgaaga agatgaggaa tgggtggtga caaaagacaa
     2101 gcccaagtat gatgagatct tctacaacct cgcaccgaat gaaggcaaac tgagtggcac
     2161 caaagccaaa gattggatgg tgagcacaag gctccccaac tctgttttgg gtcgtatctg
     2221 gaagctttcg gatgtggatc gtgacggcat gctggatgat gaggagtttg ccctggccag
     2281 ccatctgatt gaagtgaaac tggatggtca tgggctgccc cctgagctcc ctgcccgtct
     2341 cgtgccacca tccaagcgcc ggcaaaaagg ctctgatgct tagcaacttt attatacata
     2401 gttgataatt cactggccgt cgttttacgg taccatcgat gatgatccag acatgataag
     2461 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2521 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2581 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2641 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag atcagatctg
     2701 cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca
     2761 gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc
     2821 ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc
     2881 aaaggaccaa tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca
     2941 gaataaataa catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc
     3001 aagggaaaat agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat
     3061 tgtaaggagt aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta
     3121 ttgattttta attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt
     3181 aatcaagtaa aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca
     3241 agcccagcag atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     3301 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     3361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3541 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3601 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3661 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3721 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3781 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3841 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3901 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3961 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4021 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4081 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4141 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4201 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4261 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4321 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4381 aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4441 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4501 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4561 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4621 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4681 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4741 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4801 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4861 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4921 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4981 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5041 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5101 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5161 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5221 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5281 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5341 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5401 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5461 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     5521 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5581 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5641 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5701 agggcgatgg cccacgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta
     5761 ccaatgcacc aattggccac aatgacggct actacatggt gccattcctt cctctttata
     5821 ggaatggaga ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg
     5881 acccaggtca ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg
     5941 ttcagacacc catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga
     6001 cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa
     6061 attttactca attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa
     6121 agtactccat taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa
     6181 actagagatt cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg
     6241 ttttatcctg ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa
     6301 tgccagtaca cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt
     6361 ctcaaaattg ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct
     6421 gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct
     6481 gcaaatagca ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc
     6541 cctcgagcag gaaacagcta tgaccatgat tacgccaagc tatcaacttt gtatagaaaa
     6601 gttggcacgc gcagattccg tgtgggcctg gtaatatctt accattttaa gctattttaa
     6661 gattagggaa aaaaattaaa taaaactact ttaaaattac tttccttaaa aagcaacata
     6721 tttatttagt ttttgtttaa ctggctcaag attgtaatat tttactttta catgtttttt
     6781 taagtaccta aatgtgttca aaagtaaagt atgttattgt tttttttttt taatgttagc
     6841 taagctttaa attgtaatat attactttta aaagtgactt tttaaaaatg taaataaaaa
     6901 agtaactata aatgtgtaat gcatgttatt tctgtgggtt tttaattttc atctcaactg
     6961 ggcttacaat tttaacataa taaaaatcaa atcaaatcaa atcaggccct tatttgtcac
     7021 atacactctt gtatactgaa ataaactaaa tgttgagaaa aatttaaata caccaaaata
     7081 acgcatacct tttttgtaat tacctaagta aaacatttag ttcctttttt aagtgactca
     7141 agattgtaat ctattaattg taaaagataa aactagtgat caatctgacg tgcttctatg
     7201 cagcctgtga gattaattgt gatgtttaat tattaaacaa tttaaatgga tcaaacattt
     7261 cacatgtgct tattgtgtcc aacaaaacca gcagaagcac ttaaaaatat tgcatgacaa
     7321 catgagtttt taatgtaaat tatatatact tcctaaacga agttaatcat atttctaaaa
     7381 taataatgtc attgaaatat ttaaataaat gataagcttt agttgattca acataaaatt
     7441 gaaagcaata aggacttttt ttttttacag cggtgaagca aaagcatgta ataatcatat
     7501 aatataataa ataaataaat aaataatggc tcttggtgca aatatgaaca ctttaaataa
     7561 atatagaata aatatgatca ataagtcata acgatgtgtt ttatgttgat tataatttaa
     7621 acatgtttct aacgcaagtt ttaaaaactg ttttaaagta ttattttaag ttataagatc
     7681 aatttgactc aacattttgt agtgaaaata tgttgtgtaa aattaatata atatacacta
     7741 taaaaatgca gggttccaca caatacatta atgttgtccc aacacaaatc gattaagtta
     7801 acaaatatat gtggattgaa cataaaaaat taagttgtcc caatgaaatc tcaagaattg
     7861 tgttgtttca gctcatttta aataagtagt tttaacaagt aaaaaataac atatttttga
     7921 gtgtgtatat atatatatta caaaatataa taatataaaa tgtaaaagat ataaaccatg
     7981 ctacatatat ttaagagcta ttttgcacat tacttttttg caagccattt tagtgttttt
     8041 tttacaaata tatattagct aaatatacaa tatcttaaat atacaattaa tcagacatgc
     8101 aattaatgca tttaacctac ataaaaatac atgatcaaca tatgttttat gctgattaca
     8161 actaaatcaa acgtaatgaa attaatttat aaaagcgatt taaatatgtc aatattttaa
     8221 gttaaaatca cttgaaacct actataaatg aacttaaatt aaaaaaatct cctttaattc
     8281 caatgcaaca gtgttgtttt ttcagctgag gacccccggt gcagatggtt ggcccccggc
     8341 ccgctattct aaggaagcct ttcttaattt tagggcggcg ctgcatgcgc tcagatcaga
     8401 ttcacgggtg gagagggtta ttttctcttt tttatttttt cttgcctgct catatttggg
     8461 cctgtgtgaa ccccaggctc cgcctcggcc cccaggagcc gctcttattt aaaggggatc
     8521 ctcgccttca gtcttcgtcc cagtggaagc atcagtcttg tgataacaac ttggtgagtc
     8581 ctacagacca gcgctcgact ctaggcttta aatgcattga tttctctgga ttagctaccg
     8641 ggagagtttt aactttgctt attagtcttg tgctcgaact gctaggttta aagagtgttt
     8701 tctctaatgc atgcatgaag cgacagctga aaaccagaaa tagagcgttt gtcctcctgt
     8761 taaactctag cctttctttg gcatttgtca gtccgagtct tcagctgccg gcaggagatt
     8821 gggtttcagt aacacttgct gagcacaaac tacacgtgct ttcttcagat ttaattcatt
     8881 attaatcatt ctagatttaa atgatgttct tactcattgc tgattatttt agccttgcgg
     8941 agcagattaa atgagcacgt gtttcattaa gattgattta aatataatta atatttcacc
     9001 actgtagttt taaaattgcc ttctaagtca tggttgattt ttaataggta tcggctctaa
     9061 ttagattact attttggttt ttaaatgtta tttctatttc atttgtaggc tcgtttttaa
     9121 tgtgatttta ttagattcta gttttaaaat catctcttag ccttaatgag cagactgaat
     9181 gaacatgttt cattaagatt aacttcatta taatgaatta tttaatcatt ctagtgggtt
     9241 ataaatcagc tctatttaaa ttaattgtcg tgtttatttc tatttaattt ttgattattt
     9301 tttcagggtc gtttttatgt gattttttcc cgtttctaat tttaaactaa ttgtttgatt
     9361 tttatatgat tcgactcttt ttaaatgaat tgttttgctt tttttattga gcatatatat
     9421 attatacaat ttcaatcaac aattgttgtg ctttttaaat gttgtttcta tttcatttct
     9481 agggtcgatt ttattcgatt ctagttttaa aatcgtgatt tttaacttct aaccttactg
     9541 agcagactga atgaacatgt ttcatcaaga ttaacttcat tatagttaat tattgaatcg
     9601 tgctagtggt ttataaatca gctctattta aattaatcat catcattatt tctatttaat
     9661 ttttattatt tctttaggat tatttttatg tgattttttt cacgttctag ttttaaactc
     9721 aatgctcatt actgctcacc cttgctgagc agtctttcgt taagattaaa ttaattaata
     9781 accattctag ttttaaaaca gtgtcttaag gcatagctga tattttacaa gtttactatt
     9841 taatatatct cctttctttt tttagggtcg atttcatctg atttgattaa tttttattat
     9901 cttcactaag ctttttgttc taggttttat tctcagtatt attactgtta tatttgaact
     9961 gaatttctcc tcatagttta ttcattttat ttatttattt tttgtttatt cgctattttg
    10021 tattttagtt tttaattaga aattttatta gaatcgtttt gaactagttt ttatgcactc
    10081 taacattaat attaaatcat aactattatt tttattataa tttatttgtg tttatctttt
    10141 tgtgtagctt tcatgtgttt ttattttagt tttattatgt ttgcattgaa ttttttggac
    10201 tagtttatat aacgatatac agaaaagcca tgacaacaaa tgtttttata atacctatta
    10261 agtataagct aatcagattt ccactattct gttcaaagtt aacattatta gccattctga
    10321 tggatcattt gacatttgtc atatgtcttt atgaacactg ctctttcaga tgccactttt
    10381 aaagctgttt tttacagtaa atgaacgttt attattatga cattatttcc taaggcctct
    10441 agagtacagc aggatgtggc gtgtgcatta ccgtgtaatt atgctgactg taaatgtgca
    10501 tttatagact gtcagcatgt ctgactgtgc gctgttgtga ttggtcctgc acagaccaag
//
