LOCUS       sequence-224844-        9587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to Caveolin3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-Cav3-mKate2
FEATURES             Location/Qualifiers
     source          1..9587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(483..507)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             509..1207
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    1329..1410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1411..1431)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1439..1455)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1471..1605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1495..1514)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(2044..2231,2232..2243)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2277..2293)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2277..2293)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2330..2350)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2330..2348)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2369..2385)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2382..2404)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2393..2409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2417..2423,2424..2441,2442..2447))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2462..2483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2600..2617)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2771..3359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2851..2870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3530..4321,4322..4390))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4153..4172
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4391..4495)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4658..4679)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5028..5039,5040..5202)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5575..5591
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5611..5631
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggcggac cagtacaaca ctaacgagga
       61 gaagatcctg agggacagtc acaccaaaga gatcgacttg atcaacaggg accccaagca
      121 aatcaacgaa gatgtcgtga aggtggattt cgaggatgtg atcgccgagc ctgatggcac
      181 ccacagtatg gacggcgtgt ggaaggccag ttacaccacc ttcaccgtct ccaagtactg
      241 gtgctaccgt gtgctatcag ctgtctttgg catcccggtg gcactgctgt ggggcttctg
      301 cttcgcctgc atctccttct gtcacatatg ggccgtgatg ccttgcatta aaagctatct
      361 gatcgagacg caatgcctga gccgcatcta ctctctctgc atccacacct tctgtgaccc
      421 cttgtttgaa gccctaggga agatctttag cagtgttcgg gtggccttac gcaaagaggt
      481 tcacccagct ttcttgtaca aagtggctat ggtgagcgag ctgattaagg agaacatgca
      541 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
      601 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggcggtcg agggcggccc
      661 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca aaaccttcat
      721 caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccccgagg gcttcacatg
      781 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
      841 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg
      901 ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagaccc tgtaccccgc
      961 tgacggcggc ctggaaggca gagccgacat ggccctgaag ctcgtgggcg ggggccacct
     1021 gatctgcaac ttgaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc
     1081 cggcgtctac tatgtggaca gaagactgga aagaatcaag gaggccgaca aagagaccta
     1141 cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca
     1201 cagatgagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     1261 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1321 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1381 atttcacaaa taaagcattt ttttcactgc caactttatt atacatagtt gataattcac
     1441 tggccgtcgt tttacggtac catcgatgat gatccagaca tgataagata cattgatgag
     1501 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1561 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1621 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     1681 ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga agatacggcc
     1741 acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat
     1801 cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt
     1861 caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga
     1921 acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat
     1981 ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga
     2041 atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa
     2101 aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt
     2161 gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat
     2221 tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc
     2281 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     2341 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2401 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2461 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2521 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2581 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2641 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2701 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2761 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2821 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2881 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2941 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3001 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3061 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3121 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3181 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3241 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3301 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3361 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3421 gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3481 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3541 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3601 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3661 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3721 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3781 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3841 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3901 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3961 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4021 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4081 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4141 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4201 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4261 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4321 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4381 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4441 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4501 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4561 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4621 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4681 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4741 acgctggcta agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat
     4801 tggccacaat gacggctact acatggtgcc attccttcct ctttatagga atggagacta
     4861 cctcctgtcc aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg
     4921 cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat
     4981 atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa
     5041 agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt
     5101 aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa
     5161 aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt
     5221 gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt tatcctgctt
     5281 tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc cagtacacgc
     5341 tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc aaaattgtta
     5401 gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa aagcctctca
     5461 caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca aatagcagga
     5521 aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccct cgagcaggaa
     5581 acagctatga ccatgattac gccaagctat caactttgta tagaaaagtt ggcacgcgca
     5641 gattccgtgt gggcctggta atatcttacc attttaagct attttaagat tagggaaaaa
     5701 aattaaataa aactacttta aaattacttt ccttaaaaag caacatattt atttagtttt
     5761 tgtttaactg gctcaagatt gtaatatttt acttttacat gtttttttaa gtacctaaat
     5821 gtgttcaaaa gtaaagtatg ttattgtttt ttttttttaa tgttagctaa gctttaaatt
     5881 gtaatatatt acttttaaaa gtgacttttt aaaaatgtaa ataaaaaagt aactataaat
     5941 gtgtaatgca tgttatttct gtgggttttt aattttcatc tcaactgggc ttacaatttt
     6001 aacataataa aaatcaaatc aaatcaaatc aggcccttat ttgtcacata cactcttgta
     6061 tactgaaata aactaaatgt tgagaaaaat ttaaatacac caaaataacg catacctttt
     6121 ttgtaattac ctaagtaaaa catttagttc cttttttaag tgactcaaga ttgtaatcta
     6181 ttaattgtaa aagataaaac tagtgatcaa tctgacgtgc ttctatgcag cctgtgagat
     6241 taattgtgat gtttaattat taaacaattt aaatggatca aacatttcac atgtgcttat
     6301 tgtgtccaac aaaaccagca gaagcactta aaaatattgc atgacaacat gagtttttaa
     6361 tgtaaattat atatacttcc taaacgaagt taatcatatt tctaaaataa taatgtcatt
     6421 gaaatattta aataaatgat aagctttagt tgattcaaca taaaattgaa agcaataagg
     6481 actttttttt tttacagcgg tgaagcaaaa gcatgtaata atcatataat ataataaata
     6541 aataaataaa taatggctct tggtgcaaat atgaacactt taaataaata tagaataaat
     6601 atgatcaata agtcataacg atgtgtttta tgttgattat aatttaaaca tgtttctaac
     6661 gcaagtttta aaaactgttt taaagtatta ttttaagtta taagatcaat ttgactcaac
     6721 attttgtagt gaaaatatgt tgtgtaaaat taatataata tacactataa aaatgcaggg
     6781 ttccacacaa tacattaatg ttgtcccaac acaaatcgat taagttaaca aatatatgtg
     6841 gattgaacat aaaaaattaa gttgtcccaa tgaaatctca agaattgtgt tgtttcagct
     6901 cattttaaat aagtagtttt aacaagtaaa aaataacata tttttgagtg tgtatatata
     6961 tatattacaa aatataataa tataaaatgt aaaagatata aaccatgcta catatattta
     7021 agagctattt tgcacattac ttttttgcaa gccattttag tgtttttttt acaaatatat
     7081 attagctaaa tatacaatat cttaaatata caattaatca gacatgcaat taatgcattt
     7141 aacctacata aaaatacatg atcaacatat gttttatgct gattacaact aaatcaaacg
     7201 taatgaaatt aatttataaa agcgatttaa atatgtcaat attttaagtt aaaatcactt
     7261 gaaacctact ataaatgaac ttaaattaaa aaaatctcct ttaattccaa tgcaacagtg
     7321 ttgttttttc agctgaggac ccccggtgca gatggttggc ccccggcccg ctattctaag
     7381 gaagcctttc ttaattttag ggcggcgctg catgcgctca gatcagattc acgggtggag
     7441 agggttattt tctctttttt attttttctt gcctgctcat atttgggcct gtgtgaaccc
     7501 caggctccgc ctcggccccc aggagccgct cttatttaaa ggggatcctc gccttcagtc
     7561 ttcgtcccag tggaagcatc agtcttgtga taacaacttg gtgagtccta cagaccagcg
     7621 ctcgactcta ggctttaaat gcattgattt ctctggatta gctaccggga gagttttaac
     7681 tttgcttatt agtcttgtgc tcgaactgct aggtttaaag agtgttttct ctaatgcatg
     7741 catgaagcga cagctgaaaa ccagaaatag agcgtttgtc ctcctgttaa actctagcct
     7801 ttctttggca tttgtcagtc cgagtcttca gctgccggca ggagattggg tttcagtaac
     7861 acttgctgag cacaaactac acgtgctttc ttcagattta attcattatt aatcattcta
     7921 gatttaaatg atgttcttac tcattgctga ttattttagc cttgcggagc agattaaatg
     7981 agcacgtgtt tcattaagat tgatttaaat ataattaata tttcaccact gtagttttaa
     8041 aattgccttc taagtcatgg ttgattttta ataggtatcg gctctaatta gattactatt
     8101 ttggttttta aatgttattt ctatttcatt tgtaggctcg tttttaatgt gattttatta
     8161 gattctagtt ttaaaatcat ctcttagcct taatgagcag actgaatgaa catgtttcat
     8221 taagattaac ttcattataa tgaattattt aatcattcta gtgggttata aatcagctct
     8281 atttaaatta attgtcgtgt ttatttctat ttaatttttg attatttttt cagggtcgtt
     8341 tttatgtgat tttttcccgt ttctaatttt aaactaattg tttgattttt atatgattcg
     8401 actcttttta aatgaattgt tttgcttttt ttattgagca tatatatatt atacaatttc
     8461 aatcaacaat tgttgtgctt tttaaatgtt gtttctattt catttctagg gtcgatttta
     8521 ttcgattcta gttttaaaat cgtgattttt aacttctaac cttactgagc agactgaatg
     8581 aacatgtttc atcaagatta acttcattat agttaattat tgaatcgtgc tagtggttta
     8641 taaatcagct ctatttaaat taatcatcat cattatttct atttaatttt tattatttct
     8701 ttaggattat ttttatgtga tttttttcac gttctagttt taaactcaat gctcattact
     8761 gctcaccctt gctgagcagt ctttcgttaa gattaaatta attaataacc attctagttt
     8821 taaaacagtg tcttaaggca tagctgatat tttacaagtt tactatttaa tatatctcct
     8881 ttcttttttt agggtcgatt tcatctgatt tgattaattt ttattatctt cactaagctt
     8941 tttgttctag gttttattct cagtattatt actgttatat ttgaactgaa tttctcctca
     9001 tagtttattc attttattta tttatttttt gtttattcgc tattttgtat tttagttttt
     9061 aattagaaat tttattagaa tcgttttgaa ctagttttta tgcactctaa cattaatatt
     9121 aaatcataac tattattttt attataattt atttgtgttt atctttttgt gtagctttca
     9181 tgtgttttta ttttagtttt attatgtttg cattgaattt tttggactag tttatataac
     9241 gatatacaga aaagccatga caacaaatgt ttttataata cctattaagt ataagctaat
     9301 cagatttcca ctattctgtt caaagttaac attattagcc attctgatgg atcatttgac
     9361 atttgtcata tgtctttatg aacactgctc tttcagatgc cacttttaaa gctgtttttt
     9421 acagtaaatg aacgtttatt attatgacat tatttcctaa ggcctctaga gtacagcagg
     9481 atgtggcgtg tgcattaccg tgtaattatg ctgactgtaa atgtgcattt atagactgtc
     9541 agcatgtctg actgtgcgct gttgtgattg gtcctgcaca gaccaag
//
