LOCUS       sequence-22485-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Iwai H, Zuger S, Jin J, Tam PH
  TITLE     Highly efficient protein trans-splicing by a naturally split DnaE 
            intein from Nostoc punctiforme.
  JOURNAL   FEBS Lett. 2006 Mar 20. 580(7):1853-8.
  PUBMED    16516207
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2006 Mar 20. 580(7):1853-8."
COMMENT     Sequence Label: pSKDuet01
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    174..198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(199..217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(773..790)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(800..817)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    873..897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(898..916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtatagctg cgctgtagac gagtccatgt gctggcggtt caaatttcgc agcagcggtt
       61 tctttaccag actcgagggt accgacgtca gcgatcgcgt ggccggccga tatccaattg
      121 agatctgcca tatgtatatc tccttcttat acttaactaa tatactaaga tggggaattg
      181 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgattatgc ggccgtgtac
      241 aatacgatta ctttctgttc gacttaagca ttatgcggcc gcaagcttaa ttcggcaaat
      301 tatcaacccg catcaaatcc aattcacgtt caaatatttc atcaattggc aacatttgac
      361 catcaacagt cataaactta tggtcttttg ttgcccgaat caatgaacca tcttccaaac
      421 aatactcaaa cacctcttgt tctccgcgat cgtgccattg tgctacaggt tgtgtataaa
      481 tatttccatt attatcaacg ctataaacag tacattcgat gcgcttttct acaattttac
      541 caatcggtaa taatccatat tctactgtca atatttccgt ttcatagctt aaacaggatc
      601 cttccgttac ggtgtaggtt ttggtagcgt cgtcgtaggt ccattcaccg tcaacaccgt
      661 tgtcgttagc gtactgtttg aaaacttttt ccgcggtagc agcgtcaaca gcttcggtgg
      721 tggtttcacc tttcagggtt ttaccgttca ggataagttt gtactgcata tggctgccgc
      781 gcggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg gtatatctcc
      841 ttattaaagt taaacaaaat tatttctaca ggggaattgt tatccgctca caattcccct
      901 atagtgagtc gtattaattt cctaatgcag gagtcgcata agggagagcg tcgagatccc
      961 ggacacatcg aa
//
