LOCUS       sequence-22486-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Iwai H, Zuger S, Jin J, Tam PH
  TITLE     Highly efficient protein trans-splicing by a naturally split DnaE 
            intein from Nostoc punctiforme.
  JOURNAL   FEBS Lett. 2006 Mar 20. 580(7):1853-8.
  PUBMED    16516207
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2006 Mar 20. 580(7):1853-8."
COMMENT     Sequence Label: pSKBAD2
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(415..432)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      585..671
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctttttatcg cactctctac tgtttctcca tacccgtttt tttgggctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg atcaaaatag ccacacgtaa atatttaggc
      121 aaacaaaatg tctatgacat tggagttgag cgcgaccata attttgcact caaaaatggc
      181 ttcatagctt ctaattgttt caatggtacc cagtacaaac ttatcctgaa cggtaaaacc
      241 ctgaaaggtg aaaccaccac cgaagctgtt gacgctgcta ccgcggaaaa agttttcaaa
      301 cagtacgcta acgacaacgg tgttgacggt gaatggacct acgacgacgc taccaaaacc
      361 tacaccgtaa cggaataagc ttggctgttt tggcggatga gagaagattt tcagcctgat
      421 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
      481 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
      541 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
      601 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      661 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gc
//
