LOCUS       sequence-22487-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Iwai H, Zuger S, Jin J, Tam PH
  TITLE     Highly efficient protein trans-splicing by a naturally split DnaE 
            intein from Nostoc punctiforme.
  JOURNAL   FEBS Lett. 2006 Mar 20. 580(7):1853-8.
  PUBMED    16516207
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2006 Mar 20. 580(7):1853-8."
COMMENT     Sequence Label: pSZBAD43
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             48..70
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(405..422)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      575..661
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cactctctac tgtttctcca tacccgtttt tttgggctat aaataatttt gtttaacttt
       61 aagaaggaga tatacatatg gttaaagtta tcggtcgtcg atccctgggt gtgcaacgca
      121 tctttgatat cggtctgccg caagaccata attttctgct ggctaatggt gctatcgccg
      181 ccaactgtta taacggtacc cagtacaaac ttatcctgaa cggtaaaacc ctgaaaggtg
      241 aaaccaccac cgaagctgtt gacgctgcta ccgcggaaaa agttttcaaa cagtacgcta
      301 acgacaacgg tgttgacggt gaatggacct acgacgacgc taccaaaacc tacaccgtaa
      361 cggaataagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
      421 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
      481 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
      541 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
      601 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
      661 tccgccggga gcggatttga acgttgcgaa gcacggc
//
