LOCUS       sequence-22489-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Iwai H, Zuger S, Jin J, Tam PH
  TITLE     Highly efficient protein trans-splicing by a naturally split DnaE 
            intein from Nostoc punctiforme.
  JOURNAL   FEBS Lett. 2006 Mar 20. 580(7):1853-8.
  PUBMED    16516207
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2006 Mar 20. 580(7):1853-8."
COMMENT     Sequence Label: pSZBAD47
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             56..78
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(413..430)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      583..669
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagaa ataattttgt
       61 ttaactttaa gaaggagata tacatatggt taaagttatc ggtcgtcgat ccctgggtgt
      121 gcaacgcatc tttgatatcg gtctgccgca agaccataat tttctgctgg ctaatggtgc
      181 tatcgccgcc aactgttgga acggtaccca gtacaaactt atcctgaacg gtaaaaccct
      241 gaaaggtgaa accaccaccg aagctgttga cgctgctacc gcggaaaaag ttttcaaaca
      301 gtacgctaac gacaacggtg ttgacggtga atggacctac gacgacgcta ccaaaaacta
      361 caccgtaacg gaataagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
      421 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
      481 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
      541 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      601 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      661 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggccg
//
