LOCUS       sequence-224890-        9188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express BUB1-WT fused to LacI for recruitment of BUB1 to 
            LacO spots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Vleugel M, Hoek TA, Tromer E, Sliedrecht T, Groenewold V, Omerzu M, 
            Kops GJ
  TITLE     Dissecting the roles of human BUB1 in the spindle assembly 
            checkpoint.
  JOURNAL   J Cell Sci. 2015 Aug 15;128(16):2975-82. doi: 10.1242/jcs.169821. 
            Epub 2015 Jul 6.
  PUBMED    26148513
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.169821"; journalName: "J Cell Sci"; date: "2015-08-15- 
            15"; volume: "128"; issue: "16"; pages: "2975-82"
COMMENT     Sequence Label: pLacI-LAP-BUB1
FEATURES             Location/Qualifiers
     source          1..9188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(255..274)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             1316..1336
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      1355..1364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1361..1363,1364..1366,1367..2077)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1361..1363,1364..1366,1367..2077)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1406..1427)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1667..1686)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2014..2035
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2123..2143
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2156..2200
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     polyA_signal    5612..5733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5649..5668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5703..5722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5740..6195)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5877..5898)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6089..6108
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6222..6326
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6328..6685
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6328..6519
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6351..6371)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6536..6671
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6720..7514
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6720..7514
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6774..6793)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7384..7403
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7702..7721)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7746..7793
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8122..8710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8611..8630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8885..9188
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcatggtgaa
      241 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
      301 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
      361 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
      421 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
      481 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
      541 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
      601 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
      661 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
      721 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
      781 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
      841 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
      901 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
      961 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     1021 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     1081 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     1141 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     1201 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     1261 ggccgattca ttaatgcagc tggcacgaca ggtttcccga agcagcctga ggcctcctaa
     1321 gaagaagagg aaggttgggc tagcgctacc ggtcgccacc atggtgagca agggcgagga
     1381 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1441 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1501 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1561 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1621 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1681 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1741 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1801 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1861 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1921 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1981 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2041 cgccgggatc actctcggca tggacgagct gtacaagtcc ggaggggatc ggtggagctc
     2101 caccggtggc ggccgctcta gagagaatct ttattttcag ggcgccgcca aattcaaaga
     2161 aaccgctgct gctaaattcg aacgccagca catggacagc ggaggtggag gtactagtct
     2221 cgagatggac accccggaaa atgtccttca gatgcttgaa gcccacatgc agagctacaa
     2281 gggcaatgac cctcttggtg aatgggaaag atacatacag tgggtagaag agaattttcc
     2341 tgagaataaa gaatacttga taactttact agaacattta atgaaggaat ttttagataa
     2401 gaagaaatac cacaatgacc caagattcat cagttattgt ttaaaatttg ctgagtacaa
     2461 cagtgacctc catcaatttt ttgagtttct gtacaaccat gggattggaa ccctgtcatc
     2521 ccctctgtac attgcctggg cggggcatct ggaagcccaa ggagagctgc agcatgccag
     2581 tgctgtcctt cagagaggaa ttcaaaacca ggctgaaccc agagagttcc tgcaacaaca
     2641 atacaggtta tttcagacac gcctcactga aacccatttg ccagctcaag ctagaacctc
     2701 agaacctctg cataatgttc aggttttaaa tcaaatgata acatcaaaat caaatccagg
     2761 aaataacatg gcctgcattt ctaagaatca gggttcagag ctttctggag tgatatcttc
     2821 agcttgtgat aaagagtcaa atatggaacg aagagtgatc acgatttcta aatcagaata
     2881 ttctgtgcac tcatctttgg catccaaagt tgatgttgag caggttgtta tgtattgcaa
     2941 ggagaagctt attcgtgggg aatcagaatt ttcctttgaa gaattgagag cccagaaata
     3001 caatcaacgg agaaagcatg agcaatgggt aaatgaagac agacattata tgaaaaggaa
     3061 agaagcaaat gcttttgaag aacagctatt aaaacagaaa atggatgaac ttcataagaa
     3121 gttgcatcag gtggtggaga catcccatga ggatctgccc gcttcccagg aaaggtccga
     3181 ggttaatcca gcacgtatgg ggccaagtgt aggctcccag caggaactga gagcgccatg
     3241 tcttccagta acctatcagc agacaccagt gaacatggaa aagaacccaa gagaggcacc
     3301 tcctgttgtt cctcctttgg caaatgctat ttctgcagct ttggtgtccc cagccaccag
     3361 ccagagcatt gctcctcctg ttcctttgaa agcccagaca gtaacagact ccatgtttgc
     3421 agtggccagc aaagatgctg gatgtgtgaa taagagtact catgaattca agccacagag
     3481 tggagcagag atcaaagaag ggtgtgaaac acataaggtt gccaacacaa gttcttttca
     3541 cacaactcca aacacatcac tgggaatggt tcaggcaacg ccatccaaag tgcagccatc
     3601 acccaccgtg cacacaaaag aagcattagg tttcatcatg aatatgtttc aggctcctac
     3661 acttcctgat atttctgatg acaaagatga atggcaatct ctagatcaaa atgaagatgc
     3721 atttgaagcc cagtttcaaa aaaatgtaag gtcatctggg gcttggggag tcaataagat
     3781 catctcttct ttgtcatctg cttttcatgt gtttgaagat ggaaacaaag aaaattatgg
     3841 attaccacag cctaaaaata aacccacagg agccaggacc tttggagaac gctctgtcag
     3901 cagacttcct tcaaaaccaa aggaggaagt gcctcatgct gaagagtttt tggatgactc
     3961 aactgtatgg ggtattcgct gcaacaaaac cctggcaccc agtcctaaga gcccaggaga
     4021 cttcacatct gctgcacaac ttgcgtctac accattccac aagcttccag tggagtcagt
     4081 gcacatttta gaagataaag aaaatgtggt agcaaaacag tgtacccagg cgactttgga
     4141 ttcttgtgag gaaaacatgg tggtgccttc aagggatgga aaattcagtc caattcaaga
     4201 gaaaagccca aaacaggcct tgtcgtctca catgtattca gcatccttac ttcgtctgag
     4261 ccagcctgct gcaggtgggg tacttacctg tgaggcagag ttgggcgttg aggcttgcag
     4321 actcacagac actgacgctg ccattgcaga agatccacca gatgctattg ctgggctcca
     4381 agcagaatgg atgcagatga gttcacttgg gactgttgat gctccaaact tcattgttgg
     4441 gaacccatgg gatgataagc tgattttcaa acttttatct gggctttcta aaccagtgag
     4501 ttcctatcca aatacttttg aatggcaatg taaacttcca gccatcaagc ccaagactga
     4561 atttcaattg ggttctaagc tggtctatgt ccatcacctt cttggagaag gagcctttgc
     4621 ccaggtgtac gaagctaccc agggagatct gaatgatgct aaaaataaac agaaatttgt
     4681 tttaaaggtc caaaagcctg ccaacccctg ggaattctac attgggaccc agttgatgga
     4741 aagactaaag ccatctatgc agcacatgtt tatgaagttc tattctgccc acttattcca
     4801 gaatggcagt gtattagtag gagagctcta cagctatgga acattattaa atgccattaa
     4861 cctctataaa aatacccctg aaaaagtgat gcctcaaggt cttgtcatct cttttgctat
     4921 gagaatgctt tacatgattg agcaagtgca tgactgtgaa atcattcatg gagacattaa
     4981 accagacaat ttcatacttg gaaacggatt tttggaacag gatgatgaag atgatttatc
     5041 tgctggcttg gcactgattg acctgggtca gagtatagat atgaaacttt ttccaaaagg
     5101 aactatattc acagcaaagt gtgaaacatc tggttttcag tgtgttgaga tgctcagcaa
     5161 caaaccatgg aactaccaga tcgattactt tggggttgct gcaacagtat attgcatgct
     5221 ctttggcact tacatgaaag tgaaaaatga aggaggagag tgtaagcctg aaggtctttt
     5281 tagaaggctt cctcatttgg atatgtggaa tgaatttttt catgttatgt tgaatattcc
     5341 agattgtcat catcttccat ctttggattt gttaaggcaa aagctgaaga aagtatttca
     5401 acaacactat actaacaaga ttagggccct acgtaatagg ctaattgtac tgctcttaga
     5461 atgtaaacga tcacgaaaat aaggatccac cggatctaga taactgatca taatcagcca
     5521 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5581 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5641 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5701 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt
     5761 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     5821 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     5881 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     5941 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6001 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6061 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6121 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6181 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6301 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     6361 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6421 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6481 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6541 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6601 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6661 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     6721 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6781 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6841 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6901 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6961 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7021 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7081 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7141 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7201 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7261 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7321 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7381 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7441 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7501 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     7561 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7621 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7681 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     7741 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     7801 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     7861 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     7921 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     7981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     8821 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     8881 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     8941 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     9001 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     9061 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     9121 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     9181 ttaccatg
//
