LOCUS       sequence-224892-        9546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for expression of siRNA-resistant BUB1-WT from the FRT site of 
            FlpIn cells upon addition of doxycyclin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Vleugel M, Hoek TA, Tromer E, Sliedrecht T, Groenewold V, Omerzu M, 
            Kops GJ
  TITLE     Dissecting the roles of human BUB1 in the spindle assembly 
            checkpoint.
  JOURNAL   J Cell Sci. 2015 Aug 15;128(16):2975-82. doi: 10.1242/jcs.169821. 
            Epub 2015 Jul 6.
  PUBMED    26148513
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.169821"; journalName: "J Cell Sci"; date: "2015-08-15- 
            15"; volume: "128"; issue: "16"; pages: "2975-82"
COMMENT     Sequence Label: pcDNA5-LAP-BUB1
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      404..413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(410..412,413..415,416..1126)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(455..476)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(716..735)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1063..1084
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1172..1192
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1205..1249
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4884..4901)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4890..5114
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5247..5266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5398..5445
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5453..6475
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5453..6475
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6605..6726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6642..6661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6696..6715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6775..6791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6775..6791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6788..6810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6799..6815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6823..6829,6830..6847,6848..6853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6868..6889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7006..7023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7177..7765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7257..7276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7936..8727,8728..8796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8559..8578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8797..8901)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8976..8995)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9167..9546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccgagct cggatccgct agcgctaccg gtcgccacca tggtgagcaa
      421 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      481 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      541 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      601 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      661 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      721 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      781 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      841 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      901 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      961 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     1021 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1081 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gaggggatcg
     1141 gtggagctcc accggtggcg gccgctctag agagaatctt tattttcagg gcgccgccaa
     1201 attcaaagaa accgctgctg ctaaattcga acgccagcac atggacagcg gaggtggagg
     1261 tactagtctc gagatggaca ccccggaaaa tgtccttcag atgcttgaag cccacatgca
     1321 gagctacaag ggcaatgacc ctcttggtga atgggaaaga tacatacagt gggtagaaga
     1381 gaattttcct gagaataaag aatacttgat aactttacta gaacatttaa tgaaggaatt
     1441 tttagataag aagaaatacc acaatgaccc aagattcatc agttattgtt taaaatttgc
     1501 tgagtacaac agtgacctcc atcaattttt tgagtttctg tacaaccatg ggattggaac
     1561 cctgtcatcc cctctgtaca ttgcctgggc ggggcatctg gaagcccaag gagagctgca
     1621 gcatgccagt gctgtccttc agagaggaat tcaaaaccag gctgaaccca gagagttcct
     1681 gcaacaacaa tacaggttat ttcagacacg cctcactgaa acccatttgc cagctcaagc
     1741 tagaacctca gaacctctgc ataatgttca ggttttaaat caaatgataa catcaaaatc
     1801 aaatccagga aataacatgg cctgcatttc taagaatcag ggttcagagc tttctggagt
     1861 gatatcttca gcttgtgata aagagtcaaa tatggaacga agagtgatta ccatttctaa
     1921 atcagaatat tctgtgcact catctttggc atccaaagtt gatgttgagc aggttgttat
     1981 gtattgcaag gagaagctta ttcgtgggga atcagaattt tcctttgaag aattgagagc
     2041 ccagaaatac aatcaacgga gaaagcatga gcaatgggta aatgaagaca gacattatat
     2101 gaaaaggaaa gaagcaaatg cttttgaaga acagctatta aaacagaaaa tggatgaact
     2161 tcataagaag ttgcatcagg tggtggagac atcccatgag gatctgcccg cttcccagga
     2221 aaggtccgag gttaatccag cacgtatggg gccaagtgta ggctcccagc aggaactgag
     2281 agcgccatgt cttccagtaa cctatcagca gacaccagtg aacatggaaa agaacccaag
     2341 agaggcacct cctgttgttc ctcctttggc aaatgctatt tctgcagctt tggtgtcccc
     2401 agccaccagc cagagcattg ctcctcctgt tcctttgaaa gcccagacag taacagactc
     2461 catgtttgca gtggccagca aagatgctgg atgtgtgaat aagagtactc atgaattcaa
     2521 gccacagagt ggagcagaga tcaaagaagg gtgtgaaaca cataaggttg ccaacacaag
     2581 ttcttttcac acaactccaa acacatcact gggaatggtt caggcaacgc catccaaagt
     2641 gcagccatca cccaccgtgc acacaaaaga agcattaggt ttcatcatga atatgtttca
     2701 ggctcctaca cttcctgata tttctgatga caaagatgaa tggcaatctc tagatcaaaa
     2761 tgaagatgca tttgaagccc agtttcaaaa aaatgtaagg tcatctgggg cttggggagt
     2821 caataagatc atctcttctt tgtcatctgc ttttcatgtg tttgaagatg gaaacaaaga
     2881 aaattatgga ttaccacagc ctaaaaataa acccacagga gccaggacct ttggagaacg
     2941 ctctgtcagc agacttcctt caaaaccaaa ggaggaagtg cctcatgctg aagagttttt
     3001 ggatgactca actgtatggg gtattcgctg caacaaaacc ctggcaccca gtcctaagag
     3061 cccaggagac ttcacatctg ctgcacaact tgcgtctaca ccattccaca agcttccagt
     3121 ggagtcagtg cacattttag aagataaaga aaatgtggta gcaaaacagt gtacccaggc
     3181 gactttggat tcttgtgagg aaaacatggt ggtgccttca agggatggaa aattcagtcc
     3241 aattcaagag aaaagcccaa aacaggcctt gtcgtctcac atgtattcag catccttact
     3301 tcgtctgagc cagcctgctg caggtggggt acttacctgt gaggcagagt tgggcgttga
     3361 ggcttgcaga ctcacagaca ctgacgctgc cattgcagaa gatccaccag atgctattgc
     3421 tgggctccaa gcagaatgga tgcagatgag ttcacttggg actgttgatg ctccaaactt
     3481 cattgttggg aacccatggg atgataagct gattttcaaa cttttatctg ggctttctaa
     3541 accagtgagt tcctatccaa atacttttga atggcaatgt aaacttccag ccatcaagcc
     3601 caagactgaa tttcaattgg gttctaagct ggtctatgtc catcaccttc ttggagaagg
     3661 agcctttgcc caggtgtacg aagctaccca gggagatctg aatgatgcta aaaataaaca
     3721 gaaatttgtt ttaaaggtcc aaaagcctgc caacccctgg gaattctaca ttgggaccca
     3781 gttgatggaa agactaaagc catctatgca gcacatgttt atgaagttct attctgccca
     3841 cttattccag aatggcagtg tattagtagg agagctctac agctatggaa cattattaaa
     3901 tgccattaac ctctataaaa atacccctga aaaagtgatg cctcaaggtc ttgtcatctc
     3961 ttttgctatg agaatgcttt acatgattga gcaagtgcat gactgtgaaa tcattcatgg
     4021 agacattaaa ccagacaatt tcatacttgg aaacggattt ttggaacagg atgatgaaga
     4081 tgatttatct gctggcttgg cactgattga cctgggtcag agtatagata tgaaactttt
     4141 tccaaaagga actatattca cagcaaagtg tgaaacatct ggttttcagt gtgttgagat
     4201 gctcagcaac aaaccatgga actaccagat cgattacttt ggggttgctg caacagtata
     4261 ttgcatgctc tttggcactt acatgaaagt gaaaaatgaa ggaggagagt gtaagcctga
     4321 aggtcttttt agaaggcttc ctcatttgga tatgtggaat gaattttttc atgttatgtt
     4381 gaatattcca gattgtcatc atcttccatc tttggatttg ttaaggcaaa agctgaagaa
     4441 agtatttcaa caacactata ctaacaagat tagggcccta cgtaataggc taattgtact
     4501 gctcttagaa tgtaaacgat cacgaaaata aggatccacc ggatctagat aactgatcat
     4561 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4621 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aaccaaatgg ccaagggaac
     4681 cactgaagaa atgaaatatg ttctgggcca acttgttggt ctgaattctc ctaactccat
     4741 tttgaaagct gctaaaactt tatatgaaca ctatagtggt ggtgaaagtc ataattcttc
     4801 atcctccaag acttttgaaa aaaaaagggg aaaaaaatga gtcgagcatg catctagagg
     4861 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4921 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4981 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5041 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5101 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     5161 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5221 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5281 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5341 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa
     5401 gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc
     5461 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     5521 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     5581 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     5641 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     5701 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     5761 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     5821 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     5881 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     5941 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     6001 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     6061 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     6121 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     6181 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     6241 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     6301 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     6361 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     6421 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     6481 actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6541 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6601 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6661 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6721 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     8941 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9001 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9061 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9121 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     9181 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     9241 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     9301 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     9361 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     9421 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     9481 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     9541 accatg
//
