LOCUS       sequence-224893-        4928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rat Syntaxin 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner 
            RA, Wang AL, Brose N, Rhee J, Brunger AT
  TITLE     Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and 
            Munc18.
  JOURNAL   Neuron. 2017 Aug 2;95(3):591-607.e10. doi: 
            10.1016/j.neuron.2017.07.004.
  PUBMED    28772123
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2017.07"; journalName: "Neuron"; date: "2017-08-2-
            2"; volume: "95"; issue: "3"; pages: "591-607.e10"
COMMENT     Sequence Label: pDC216
FEATURES             Location/Qualifiers
     source          1..4928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     175..194
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        175..193
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    194..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             312..332
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      1207..1254
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1477..1496)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1502..1521)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        1563..1654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1655..1723,1724..2515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1873..1892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             2598..3680
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2617..2636)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      3766..4354
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4255..4274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4508..4525
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4623..4640
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4624..4640
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt
       61 tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg
      121 ttatcagtat ttattatcat ttagaataaa ttttgtgtcg cccttccgcg aaattaatac
      181 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      241 acttttagga ggtaaaacat atgagctatt ggcaccatca ccaccaccac gactacgata
      301 tcccgaccag cgagaatctg tattttcagg gtaaagatcg tacccaggag ctgcgtaccg
      361 cgaaagacag cgacgatgat gacgacgtga ccgttacggt cgatcgtgac cgcttcatgg
      421 acgaattctt cgagcaagtc gaagagatcc gcggctttat tgataagatc gcagagaacg
      481 tggaagaggt taagcgcaaa cactccgcga ttctggcaag cccgaatcca gacgaaaaga
      541 cgaaagaaga actggaagag ttgatgtccg acattaagaa aaccgcgaac aaagttcgta
      601 gcaagctgaa atctatcgag cagagcatcg aacaggaaga gggtctgaac cgctccagcg
      661 ccgatctgcg tattcgtaaa acgcagcaca gcactctgag ccgcaaattc gtcgaagtca
      721 tgagcgagta caatgcaacc caaagcgact accgcgagcg cagcaaaggc cgtattcaac
      781 gtcaactgga gatcaccggt cgtaccacca cctctgaaga gttggaagat atgctggaga
      841 gcggtaaccc ggcgattttt gcgtctggca tcatcatgga cagcagcatc agcaagcaag
      901 cgctgtgcga aattgaaacg cgccatagcg aaatcattaa actggagaac agcattcgtg
      961 agttgcatga tatgttcatg gacatggcaa tgctggttga gagccagggt gagatgatcg
     1021 atcgtattga atacaatgtg gagcatgccg ttgattatgt cgagcgtgcg gtgagcgaca
     1081 cgaagaaggc cgttaagtac cagtcgaaag ctcgtcgtaa gaagatcatg attatcatct
     1141 ctagcgtgat tctgggtatt atcattgctt ccacgatcgg tggcattttt ggctaactcg
     1201 agccccctag cataacccct tggggcctct aaacgggtct tgaggggttt tttgcccctg
     1261 agacgcgtca atcgagttcg tacctaaggg cgacacccca taattagccc gggcgaaagg
     1321 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca
     1381 tggggagtcc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     1441 tcaggtggga ccaccgcgct actgccgcca ggcaaacaag gggtgttatg agccatattc
     1501 aggtataaat gggctcgcga taatgttcag aattggttaa ttggttgtaa cactgacccc
     1561 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1621 ataaatgctt caataatatt gaaaaaggaa gaatatgagt attcaacatt tccgtgtcgc
     1681 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1741 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1801 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1861 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1921 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1981 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2041 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2101 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2161 agccatacca aacgacgagc gtgacaccac gatgcctgta gcgatggcaa caacgttgcg
     2221 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2281 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2341 tgctgataaa tccggagccg gtgagcgtgg ttctcgcggt atcatcgcag cgctggggcc
     2401 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2461 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaagcggc
     2521 gcgccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
     2581 aattcagggt ggtgaatatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
     2641 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
     2701 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
     2761 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
     2821 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
     2881 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
     2941 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
     3001 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
     3061 ccatcaacag tattattttc tcccatgagg acggtacgcg actgggcgtg gagcatctgg
     3121 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
     3181 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
     3241 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
     3301 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
     3361 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
     3421 ataccgaaga tagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
     3481 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     3541 gcaatcagct gttgccagtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
     3601 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3661 gactggaaag cgggcagtga ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc
     3721 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3781 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3841 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3901 accaaatact gttcttctag tgtagccgta gttagcccac cacttcaaga actctgtagc
     3961 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4021 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4081 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4141 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4201 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4261 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4321 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4381 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4441 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4501 cgagcgcagc gagtcagtga gcgaggaagc ggaaggcgag agtagggaac tgccaggcat
     4561 caaactaagc agaaggcccc tgacggatgg cctttttgcg tttctacaaa ctctttctgt
     4621 gttgtaaaac gacggccagt cttaagctcg ggccccctgg gcggttctga taacgagtaa
     4681 tcgttaatcc gcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt
     4741 agggaaagag catttgtcag aatatttaag ggcgcctgtc actttgcttg atatatgaga
     4801 attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata cgttgctttt
     4861 tcgattgatg aacacctata attaaactat tcatctatta tttatgattt tttgtatata
     4921 caatattt
//
