LOCUS       sequence-224895-        2060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Improved genetic encoded calcium sensor based on GCaMP6m.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Yang Y, Liu N, He Y, Liu Y, Ge L, Zou L, Song S, Xiong W, Liu X
  TITLE     Improved calcium sensor GCaMP-X overcomes the calcium channel 
            perturbations induced by the calmodulin in GCaMP.
  JOURNAL   Nat Commun. 2018 Apr 17;9(1):1504. doi: 10.1038/s41467-018-03719-6.
  PUBMED    29666364
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-04-17"; volume: "9(1):1504. doi"; pages: " 
            10.1038/s41467-018-03719-6"
COMMENT     Sequence Label: AAV-syn-GCaMP6m-XC
FEATURES             Location/Qualifiers
     source          1..2060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(389..397,398..415,416..418,419..451,452..454,455..478,
                     479..505,506..562,563..568,569..838,839..856,857..1291,
                     1292..1297,1298..1738)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6m
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKGSYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     775..796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(902..923)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1163..1182)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(1849..1869)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
       61 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
      121 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
      181 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
      241 ctgagagcgc agtcgagaag gtaccggatc cgccaccatg ggcatggacg agctgtacaa
      301 gggtaccgct gcaaccaaaa ttcaggctgc cttccgtgga cacataacaa ggaaaaagct
      361 caaagacgag aagaagggtg ctgaattcat gggttctcat catcatcatc atcatggtat
      421 ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga
      481 tctcgccacc atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag
      541 agctataggt cggctgagct cactcgagaa cgtctatatc aaggccgaca agcagaagaa
      601 cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
      661 ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      721 ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt
      781 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
      841 cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      901 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga
      961 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1021 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1081 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca
     1141 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1201 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1261 caacatcctg gggcacaagc tggagtacaa cctgccggac caactgactg aagagcagat
     1321 cgcagaattt aaagaggctt tctccctatt tgacaaggac ggggatggga caataacaac
     1381 caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag cagagctgca
     1441 ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc ctgagttcct
     1501 gacaatgatg gcaagaaaag ggagctacag ggacacggaa gaagaaatta gagaagcgtt
     1561 cggtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc gccacgtgat
     1621 gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca gggaagcaga
     1681 catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga cagcgaagct
     1741 tgctcttaag ttggctggac ttgatattgg ttcttaagat atcaagctta tcgataatca
     1801 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1861 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1921 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1981 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2041 gggcattgcc accacctgtc
//
