LOCUS       sequence-224900-       10152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site 
            of FlpIn cells upon addition of doxycyclin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Vleugel M, Hoek TA, Tromer E, Sliedrecht T, Groenewold V, Omerzu M, 
            Kops GJ
  TITLE     Dissecting the roles of human BUB1 in the spindle assembly 
            checkpoint.
  JOURNAL   J Cell Sci. 2015 Aug 15;128(16):2975-82. doi: 10.1242/jcs.169821. 
            Epub 2015 Jul 6.
  PUBMED    26148513
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.169821"; journalName: "J Cell Sci"; date: "2015-08-15- 
            15"; volume: "128"; issue: "16"; pages: "2975-82"
COMMENT     Sequence Label: pcDNA5-MIS12-LAP-BUB1
FEATURES             Location/Qualifiers
     source          1..10152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      398..407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(404..406,407..409,410..1120)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(449..470)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(710..729)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1057..1078
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1166..1186
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1199..1243
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(5490..5507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5496..5720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5853..5872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6004..6051
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6059..7081
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6059..7081
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7211..7332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7248..7267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7302..7321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7381..7397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7381..7397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7394..7416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7405..7421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7429..7435,7436..7453,7454..7459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7474..7495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7612..7629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7783..8371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7863..7882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8542..9333,9334..9402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9165..9184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9403..9507)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9582..9601)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9773..10152
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccgagct cgctagcgct accggtcgcc accatggtga gcaagggcga
      421 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      481 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      541 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
      601 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      661 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      721 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      781 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      841 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      901 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      961 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     1021 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1081 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggagggg atcggtggag
     1141 ctccaccggt ggcggccgct ctagagagaa tctttatttt cagggcgccg ccaaattcaa
     1201 agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcggaggtg gaggtactag
     1261 ttctgtagac ccaatgacct acgaggccca gttctttggc ttcacgccac aaacgtgcat
     1321 gcttcggatc tacattgcat ttcaagacta cctatttgaa gtgatgcagg ccgttgaaca
     1381 ggttattctg aagaagctgg atggcatccc agactgtgac attagcccag tgcagattcg
     1441 caaatgcaca gagaagtttc tttgcttcat gaaaggacat tttgataacc tttttagcaa
     1501 aatggagcaa ctgtttttgc agctgatttt acgtattccc tcaaacatct tgcttcctga
     1561 agataaatgt aaggagacac cttatagtga ggaagatttt cagcatctcc agaaagaaat
     1621 tgaacagtta caggagaagt acaagactga attatgtact aagcaggccc ttcttgcaga
     1681 attagaagag caaaaaattg ttcaggccaa actcaaacag acgttgactt tctttgatga
     1741 gcttcataat gttggcagag atcatgggac tagtgatttt agggagagtt tagtatccct
     1801 ggttcagaac tccagaaaac tacagaacat tagagacaat gtggaaaagg aatcgaaacg
     1861 actgaaaata tctctcgaga tggacacccc ggaaaatgtc cttcagatgc ttgaagccca
     1921 catgcagagc tacaagggca atgaccctct tggtgaatgg gaaagataca tacagtgggt
     1981 agaagagaat tttcctgaga ataaagaata cttgataact ttactagaac atttaatgaa
     2041 ggaattttta gataagaaga aataccacaa tgacccaaga ttcatcagtt attgtttaaa
     2101 atttgctgag tacaacagtg acctccatca attttttgag tttctgtaca accatgggat
     2161 tggaaccctg tcatcccctc tgtacattgc ctgggcgggg catctggaag cccaaggaga
     2221 gctgcagcat gccagtgctg tccttcagag aggaattcaa aaccaggctg aacccagaga
     2281 gttcctgcaa caacaataca ggttatttca gacacgcctc actgaaaccc atttgccagc
     2341 tcaagctaga acctcagaac ctctgcataa tgttcaggtt ttaaatcaaa tgataacatc
     2401 aaaatcaaat ccaggaaata acatggcctg catttctaag aatcagggtt cagagctttc
     2461 tggagtgata tcttcagctt gtgataaaga gtcaaatatg gaacgaagag tgattaccat
     2521 ttctaaatca gaatattctg tgcactcatc tttggcatcc aaagttgatg ttgagcaggt
     2581 tgttatgtat tgcaaggaga agcttattcg tggggaatca gaattttcct ttgaagaatt
     2641 gagagcccag aaatacaatc aacggagaaa gcatgagcaa tgggtaaatg aagacagaca
     2701 ttatatgaaa aggaaagaag caaatgcttt tgaagaacag ctattaaaac agaaaatgga
     2761 tgaacttcat aagaagttgc atcaggtggt ggagacatcc catgaggatc tgcccgcttc
     2821 ccaggaaagg tccgaggtta atccagcacg tatggggcca agtgtaggct cccagcagga
     2881 actgagagcg ccatgtcttc cagtaaccta tcagcagaca ccagtgaaca tggaaaagaa
     2941 cccaagagag gcacctcctg ttgttcctcc tttggcaaat gctatttctg cagctttggt
     3001 gtccccagcc accagccaga gcattgctcc tcctgttcct ttgaaagccc agacagtaac
     3061 agactccatg tttgcagtgg ccagcaaaga tgctggatgt gtgaataaga gtactcatga
     3121 attcaagcca cagagtggag cagagatcaa agaagggtgt gaaacacata aggttgccaa
     3181 cacaagttct tttcacacaa ctccaaacac atcactggga atggttcagg caacgccatc
     3241 caaagtgcag ccatcaccca ccgtgcacac aaaagaagca ttaggtttca tcatgaatat
     3301 gtttcaggct cctacacttc ctgatatttc tgatgacaaa gatgaatggc aatctctaga
     3361 tcaaaatgaa gatgcatttg aagcccagtt tcaaaaaaat gtaaggtcat ctggggcttg
     3421 gggagtcaat aagatcatct cttctttgtc atctgctttt catgtgtttg aagatggaaa
     3481 caaagaaaat tatggattac cacagcctaa aaataaaccc acaggagcca ggacctttgg
     3541 agaacgctct gtcagcagac ttccttcaaa accaaaggag gaagtgcctc atgctgaaga
     3601 gtttttggat gactcaactg tatggggtat tcgctgcaac aaaaccctgg cacccagtcc
     3661 taagagccca ggagacttca catctgctgc acaacttgcg tctacaccat tccacaagct
     3721 tccagtggag tcagtgcaca ttttagaaga taaagaaaat gtggtagcaa aacagtgtac
     3781 ccaggcgact ttggattctt gtgaggaaaa catggtggtg ccttcaaggg atggaaaatt
     3841 cagtccaatt caagagaaaa gcccaaaaca ggccttgtcg tctcacatgt attcagcatc
     3901 cttacttcgt ctgagccagc ctgctgcagg tggggtactt acctgtgagg cagagttggg
     3961 cgttgaggct tgcagactca cagacactga cgctgccatt gcagaagatc caccagatgc
     4021 tattgctggg ctccaagcag aatggatgca gatgagttca cttgggactg ttgatgctcc
     4081 aaacttcatt gttgggaacc catgggatga taagctgatt ttcaaacttt tatctgggct
     4141 ttctaaacca gtgagttcct atccaaatac ttttgaatgg caatgtaaac ttccagccat
     4201 caagcccaag actgaatttc aattgggttc taagctggtc tatgtccatc accttcttgg
     4261 agaaggagcc tttgcccagg tgtacgaagc tacccaggga gatctgaatg atgctaaaaa
     4321 taaacagaaa tttgttttaa aggtccaaaa gcctgccaac ccctgggaat tctacattgg
     4381 gacccagttg atggaaagac taaagccatc tatgcagcac atgtttatga agttctattc
     4441 tgcccactta ttccagaatg gcagtgtatt agtaggagag ctctacagct atggaacatt
     4501 attaaatgcc attaacctct ataaaaatac ccctgaaaaa gtgatgcctc aaggtcttgt
     4561 catctctttt gctatgagaa tgctttacat gattgagcaa gtgcatgact gtgaaatcat
     4621 tcatggagac attaaaccag acaatttcat acttggaaac ggatttttgg aacaggatga
     4681 tgaagatgat ttatctgctg gcttggcact gattgacctg ggtcagagta tagatatgaa
     4741 actttttcca aaaggaacta tattcacagc aaagtgtgaa acatctggtt ttcagtgtgt
     4801 tgagatgctc agcaacaaac catggaacta ccagatcgat tactttgggg ttgctgcaac
     4861 agtatattgc atgctctttg gcacttacat gaaagtgaaa aatgaaggag gagagtgtaa
     4921 gcctgaaggt ctttttagaa ggcttcctca tttggatatg tggaatgaat tttttcatgt
     4981 tatgttgaat attccagatt gtcatcatct tccatctttg gatttgttaa ggcaaaagct
     5041 gaagaaagta tttcaacaac actatactaa caagattagg gccctacgta ataggctaat
     5101 tgtactgctc ttagaatgta aacgatcacg aaaataagga tccaccggat ctagataact
     5161 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5221 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaacc aaatggccaa
     5281 gggaaccact gaagaaatga aatatgttct gggccaactt gttggtctga attctcctaa
     5341 ctccattttg aaagctgcta aaactttata tgaacactat agtggtggtg aaagtcataa
     5401 ttcttcatcc tccaagactt ttgaaaaaaa aaggggaaaa aaatgagtcg agcatgcatc
     5461 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5521 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5581 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5641 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5701 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5761 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5821 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5881 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5941 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac
     6001 ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa
     6061 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     6121 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     6181 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     6241 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     6301 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     6361 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     6421 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     6481 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     6541 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     6601 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     6661 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     6721 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     6781 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     6841 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     6901 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     6961 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     7021 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     7081 gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7141 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7201 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7261 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7321 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7381 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7441 cggaagcata aagtgtaaag cctgtggtgc ctaatgagtg agctaactca cattaattgc
     7501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8401 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8461 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8521 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8581 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctactatacg
     8641 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8701 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8761 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8821 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8881 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8941 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9001 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9061 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9121 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9181 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9241 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9301 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9361 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9421 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9541 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     9601 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     9661 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     9721 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     9781 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     9841 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     9901 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     9961 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
    10021 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
    10081 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
    10141 gctattacca tg
//
