LOCUS       sequence-22495-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Martins LM, Turk BE, Cowling V, Borg A, Jarrell ET, Cantley LC, 
            Downward J
  TITLE     Binding specificity and regulation of the serine protease and PDZ 
            domains of HtrA2/Omi.
  JOURNAL   J Biol Chem. 2003 Dec 5. 278(49):49417-27.
  PUBMED    14512424
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Dec 5. 278(49):49417-27."
COMMENT     Sequence Label: pGEX2TK-PDZ(HtrA2)
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(425..447)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(592..610)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        678..782
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctggttccgc gtggatctcg tcgtgcatct gttggatcct cctccggaat cagtgggtcc
       61 cagcggcgct acattggggt gatgatgctg accctgagtc ccagcatcct tgctgaacta
      121 cagcttcgag aaccaagctt tcccgatgtt cagcatggtg tactcatcca taaagtcatc
      181 ctgggctccc ctgcacaccg ggctggtctg cggcctggtg atgtgatttt ggccattggg
      241 gagcagatgg tacaaaatgc tgaagatgtt tatgaagctg ttcgaaccca atcccagttg
      301 gcagtgcaga tccggcgggg acgagaaaca ctgaccttat atgtgacccc tgaggtcaca
      361 gaatgagaat tcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg
      421 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      481 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
      541 tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga
      601 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      661 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      721 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      781 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      841 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      901 cacgagtggg ttac
//
