LOCUS       sequence-224971-        9463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human RNF169 and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-RNF169
FEATURES             Location/Qualifiers
     source          1..9463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        44..292
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     44..64
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     215..234
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        314..389
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        424..803
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        805..1082
                     /label=chicken beta-actin promoter
     intron          1083..2094
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2018..2040
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2107..2126
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2107..2125
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2127..2136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2131..2140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4277..4293)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4313..4537
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          4758..5696
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5653..5673
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5731..5740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5737..5739,5740..5742,5743..6456)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(5782..5803)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    6487..6598
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6716..6733)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6716..6732)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6725..6747)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6874..7329
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6961..6980)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7171..7192
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7355..7459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7460..7528,7529..8320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7678..7697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8491..9079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8980..8999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9233..9250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9367..9388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9403..9408,9409..9426,9427..9433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9441..9457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9446..9463,1..5)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 acaggaaaca gctatgacca tgaggcgcgc cccccccttc accgagggcc tatttcccat
       61 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
      121 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
      181 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
      241 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgactccag
      301 tctttctaga agagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      361 aacttgaaaa agtggcaccg agtcggtgct ttttttgatc caagggtggg gcgcgccgga
      421 ttcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      481 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      541 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      601 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      661 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      721 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      781 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      841 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      901 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      961 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     1021 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     1081 cgggagtcgc tgcgtcgcgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     1141 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     1201 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
     1261 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
     1321 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1381 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1441 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1501 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1561 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1621 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1681 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1741 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1801 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1861 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1921 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1981 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     2041 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagatcctt
     2101 aattaataat acgactcact ataggggcca ccatggcggc tgcaggtccg agtactcggg
     2161 cctcttccgc ggcggcagca gccgctctga gtcggcgggg ccggcggggc cgctgtgacg
     2221 agacggcggc agctaagact ggggccccag gcccggcttc tggaccttcg ctgttggtgt
     2281 tgtcgccgcc gttgctgcag ccgccgctgc cgccgcggcc ggaggaatcg ggctgcgccg
     2341 ggtgcctgga gccccccgga gaagcagcgg ccctgccgtg cggccactcg ctttgccgag
     2401 gctgcgccca acgcgccgcc gacgcggcgg gcccgggttg ccctcgctgc cgcgcccgcg
     2461 gcccaggctg ggcccgccgt cgggcccgcg acgacggcca ggccgactca gaggtgctgg
     2521 gcgagtgcgc ccgccgcagc caacccgagc gctgccgccc gcgccgggac gggggcgcgg
     2581 ctgccgcggg gcccaggcca gagcaggagc cgcgtgccgc gcctgcggag ccagacttta
     2641 tattcagagc accaatcaaa ttaagcaagc ctggggaact tcgtgaggaa tatgaaagct
     2701 tgagaaagct gagagaagaa aagttacaag aggaaaaacc ctctgaagat caaatccaca
     2761 agctgttacc agaggataca gaaacaggga aaaggaaaat ggatgaacag aaaaaaagag
     2821 atgaaccatt agtactgaaa acaaatctgg aacgttgtcc tgcacgtctc tcagattcag
     2881 agaatgaaga accttctcga ggccagatga cacagacaca tcgctcggca tttgtttcca
     2941 agaacaactc ctactcctta gctttcctgg cagggaagct aaactccaag gtggaaagga
     3001 gtcagagctg tagtgacaca gcccaggaaa gagcgaagag cagagtcaga gcagttccag
     3061 gcaacaaagc caaggtcaca actatgactc cagcctccaa ccccatcatt ggtgtcctct
     3121 tgtcaactca aaacaaccgc tgcgtctcgg cccctgactt aaccatcgaa aagcgtctac
     3181 ccttcagctc cctttcatcc ttggcttccc tgcataagcc agagcgttct gtcagccctg
     3241 agagcaatga cagcatctcc gaagaactaa accatttcaa gcccattgtc tgctcaccat
     3301 gtactcctcc caagagactc cctgatggcc gtgtgctaag tcctctcatc atcaaatcaa
     3361 ctccacgcaa cctaaacaga agcctgcaga agcagacttc ttatgaggcc agtccacgga
     3421 tcctcaaaaa gtgggaacag atctttcagg agcggcagat caaaaagacc ctttcaaaag
     3481 ccactcttac ctctctggct cctgaaatgg gggaagagtt actaggctct gaaggtatcc
     3541 attctagcaa ggagaagcca cttgtggctg taaatacaag attatctggt gggcaggtcc
     3601 tctctgagta tactggaccc acctctgctg atcttgatca tttcccctct gttagccaaa
     3661 caaaagcaga acaggacagt gataataaaa gtagcactga gatcccactg gaaacctgct
     3721 gttcctcaga actcaaaggg ggaggcagtg ggacttcttt ggagagggag cagtttgagg
     3781 ggttagggtc aactccagat gccaagttag acaaaacctg tataagcaga gccatgaaaa
     3841 tcaccacagt taattcagtg ctaccccaaa acagtgtttt gggtggagtc ctcaaaacaa
     3901 agcaacaatt gaagacatta aatcattttg atctgactaa tggtgttcta gttgagagcc
     3961 taagtgaaga gccacttcct tctttgcgtc gaggccggaa aagacactgc aagaccaagc
     4021 acttagaaca aaatggctcc cttaaaaaac tgcgacaaac cagtggggag gtgggtctgg
     4081 ccccaacaga cccagtcctg cgagagatgg agcagaagct tcagcaagag gaagaagacc
     4141 gacagttggc tctgcagttg cagcgcatgt tcgacaatga gaggcggact gtgagccggc
     4201 gaaaaggaag tgtggatcag tatctcctac ggtccagcaa catggccggg gccaagtaga
     4261 cgcgtaaatg attgcagatc cactagttct agagctcgct gatcagcctc gactgtgcct
     4321 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4381 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4441 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4501 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4561 tggggctcga gatccactag ttctagcctc gaggctagag cggccccggt gcctagagaa
     4621 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     4681 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     4741 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     4801 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     4861 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     4921 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     4981 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     5041 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     5101 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     5161 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     5221 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     5281 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     5341 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     5401 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     5461 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     5521 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     5581 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     5641 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     5701 cgtgagcgat cgcatcgaca gtgtttaaac gccaccatgg tgagcaaggg cgaggagctg
     5761 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5821 agcgtgaggg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     5881 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagccacggc
     5941 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6001 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg cacctacaag
     6061 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6121 gtcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
     6181 cacaacatct atatcatggc cgtcaagcag aagaacggca tcaaggtgaa cttcaagatc
     6241 cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6301 atcggcgacg gccccgtgct gctgcccgac agccactacc tgagcaccca gtccgtgctg
     6361 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttccg caccgccgcc
     6421 gggatcactc tcggcatgga cgagctgtac aagtgagaat tcctagagct cgctgatcag
     6481 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6541 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6601 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6661 aggattggga agagaatagc aggcatgcgt ttaaacatta gtcgacaggg ccgccactgg
     6721 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6781 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6841 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     6901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6961 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7021 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7081 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7141 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7201 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7261 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7321 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7681 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7981 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8161 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9241 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9301 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9361 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9421 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cac
//
