LOCUS       sequence-224974-       12542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human BRCA1 and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 12542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-BRCA1
FEATURES             Location/Qualifiers
     source          1..12542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        38..417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..696
                     /label=chicken beta-actin promoter
     intron          697..1708
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1632..1654
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1721..1740
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1721..1739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1741..1750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7356..7372)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7392..7616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          7837..8775
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8732..8752
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8810..8819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8816..8818,8819..8821,8822..9535)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(8861..8882)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    9566..9677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9795..9812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9795..9811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9804..9826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9953..10408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10040..10059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10250..10271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10434..10538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10539..10607,10608..11399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10757..10776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11570..12158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12059..12078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12312..12329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12446..12467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12482..12487,12488..12505,12506..12512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12520..12536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(12525..12542,1..5)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 acaggaaaca gctatgacca tgaggcgcgc cggattcgac attgattatt gactagttat
       61 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      121 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      181 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      241 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      301 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      361 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      421 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      481 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      541 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtc gcgccttcgc
      721 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      781 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      841 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      901 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      961 cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1021 gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1081 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     1141 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
     1201 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1261 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1321 cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     1381 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     1441 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg gcgaagcggt
     1501 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1561 cccttctcca tctccagcct cggggctgcc gcagggggac ggctgccttc gggggggacg
     1621 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     1681 tgttcatgcc ttcttctttt tcctacagat ccttaattaa taatacgact cactataggg
     1741 gccaccatgg atttatctgc tcttcgcgtt gaagaagtac aaaatgtcat taatgctatg
     1801 cagaaaatct tagagtgtcc catctgtctg gagttgatca aggaacctgt ctccacaaag
     1861 tgtgaccaca tattttgcaa attttgcatg ctgaaacttc tcaaccagaa gaaagggcct
     1921 tcacagtgtc ctttatgtaa gaatgatata accaaaagga gcctacaaga aagtacgaga
     1981 tttagtcaac ttgttgaaga gctattgaaa atcatttgtg cttttcagct tgacacaggt
     2041 ttggagtatg caaacagcta taattttgca aaaaaggaaa ataactctcc tgaacatcta
     2101 aaagatgaag tttctatcat ccaaagtatg ggctacagaa accgtgccaa aagacttcta
     2161 cagagtgaac ccgaaaatcc ttccttgcag gaaaccagtc tcagtgtcca actctctaac
     2221 cttggaactg tgagaactct gaggacaaag cagcggatac aacctcaaaa gacgtctgtc
     2281 tacattgaat tgggatctga ttcttctgaa gataccgtta ataaggcaac ttattgcagt
     2341 gtgggagatc aagaattgtt acaaatcacc cctcaaggaa ccagggatga aatcagtttg
     2401 gattctgcaa aaaaggctgc ttgtgaattt tctgagacgg atgtaacaaa tactgaacat
     2461 catcaaccca gtaataatga tttgaacacc actgagaagc gtgcagctga gaggcatcca
     2521 gaaaagtatc agggtagttc tgtttcaaac ttgcatgtgg agccatgtgg cacaaatact
     2581 catgccagct cattacagca tgagaacagc agtttattac tcactaaaga cagaatgaat
     2641 gtagaaaagg ctgaattctg taataaaagc aaacagcctg gcttagcaag gagccaacat
     2701 aacagatggg ctggaagtaa ggaaacatgt aatgataggc ggactcccag cacagaaaaa
     2761 aaggtagatc tgaatgctga tcccctgtgt gagagaaaag aatggaataa gcagaaactg
     2821 ccatgctcag agaatcctag agatactgaa gatgttcctt ggataacact aaatagcagc
     2881 attcagaaag ttaatgagtg gttttccaga agtgatgaac tgttaggttc tgatgactca
     2941 catgatgggg agtctgaatc aaatgccaaa gtagctgatg tattggacgt tctaaatgag
     3001 gtagatgaat attctggttc ttcagagaaa atagacttac tggccagtga tcctcatgag
     3061 gctttaatat gtaaaagtga aagagttcac tccaaatcag tagagagtaa tattgaagac
     3121 aaaatatttg ggaaaaccta tcggaagaag gcaagcctcc ccaacttaag ccatgtaact
     3181 gaaaatctaa ttataggagc atttgttact gagccacaga taatacaaga gcgtcccctc
     3241 acaaataaat taaagcgtaa aaggagacct acatcaggcc ttcatcctga ggattttatc
     3301 aagaaagcag atttggcagt tcaaaagact cctgaaatga taaatcaggg aactaaccaa
     3361 acggagcaga atggtcaagt gatgaatatt actaatagtg gtcatgagaa taaaacaaaa
     3421 ggtgattcta ttcagaatga gaaaaatcct aacccaatag aatcactcga aaaagaatct
     3481 gctttcaaaa cgaaagctga acctataagc agcagtataa gcaatatgga actcgaatta
     3541 aatatccaca attcaaaagc acctaaaaag aataggctga ggaggaagtc ttctaccagg
     3601 catattcatg cgcttgaact agtagtcagt agaaatctaa gcccacctaa ttgtactgaa
     3661 ttgcaaattg atagttgttc tagcagtgaa gagataaaga aaaaaaagta caaccaaatg
     3721 ccagtcaggc acagcagaaa cctacaactc atggaaggta aagaacctgc aactggagcc
     3781 aagaagagta acaagccaaa tgaacagaca agtaaaagac atgacagtga tactttccca
     3841 gagctgaagt taacaaatgc acctggttct tttactaagt gttcaaatac cagtgaactt
     3901 aaagaatttg tcaatcctag ccttccaaga gaagaaaaag aagagaaact agaaacagtt
     3961 aaagtgtcta ataatgctga agaccccaaa gatctcatgt taagtggaga aagggttttg
     4021 caaactgaaa gatctgtaga gagtagcagt atttcactgg tacctggtac tgattatggc
     4081 actcaggaaa gtatctcgtt actggaagtt agcactctag ggaaggcaaa aacagaacca
     4141 aataaatgtg tgagtcagtg tgcagcattt gaaaacccca agggactaat tcatggttgt
     4201 tccaaagata atagaaatga cacagaaggc tttaagtatc cattgggaca tgaagttaac
     4261 cacagtcggg aaacaagcat agaaatggaa gaaagtgaac ttgatgctca gtatttgcag
     4321 aatacattca aggtttcaaa gcgccagtca tttgctctgt tttcaaatcc aggaaatgca
     4381 gaagaggaat gtgcaacatt ctctgcccac tctgggtcct taaagaaaca aagtccaaaa
     4441 gtcacttttg aatgtgaaca aaaggaagaa aatcaaggaa agaatgagtc taatatcaag
     4501 cctgtacaga cagttaatat cactgcaggc tttcctgtgg ttggtcagaa agataagcca
     4561 gttgataatg ccaaatgtag tatcaaagga ggctctaggt tttgtctatc atctcagttc
     4621 agaggcaacg aaactggact cattactcca aataaacatg gacttttaca aaacccatat
     4681 cgtataccac cactttttcc catcaagtca tttgttaaaa ctaaatgtaa gaaaaatctg
     4741 ctagaggaaa actttgagga acattcaatg tcacctgaaa gagaaatggg aaatgagaac
     4801 attccaagta cagtgagcac aattagccgt aataacatta gagaaaatgt ttttaaagga
     4861 gccagctcaa gcaatattaa tgaagtaggt tccagtacta atgaagtggg ctccagtatt
     4921 aatgaaatag gttccagtga tgaaaacatt caagcagaac taggtagaaa cagagggcca
     4981 aaattgaatg ctatgcttag attaggggtt ttgcaacctg aggtctataa acaaagtctt
     5041 cctggaagta attgtaagca tcctgaaata aaaaagcaag aatatgaaga agtagttcag
     5101 actgttaata cagatttctc tccatatctg atttcagata acttagaaca gcctatggga
     5161 agtagtcata catctcaggt ttgttctgag acacctgatg acctgttaga tgatggtgaa
     5221 ataaaggaag atactagttt tgctgaaaat gacattaagg aaagttctgc tgtttttagc
     5281 aaaagcgtcc agagaggaga gcttagcagg agtcctagcc ctttcaccca tacacatttg
     5341 gctcagggtt accgaagagg ggccaagaaa ttagagtcct cagaagagaa cttatctagt
     5401 gaggatgaag agcttccctg cttccaacac ttgttatttg gtaaagtaaa caatatacct
     5461 tctcagtcta ctaggcatag caccgttgct accgagtgtc tgtctaagaa cacagaggag
     5521 aatttattat cattgaagaa tagcttaaat gactgcagta accaggtaat attggcaaag
     5581 gcatctcagg aacatcacct tagtgaggaa acaaaatgtt ctgctagctt gttttcttca
     5641 cagtgcagtg aattggaaga cttgactgca aatacaaaca cccaggatcc tttcttgatt
     5701 ggttcttcca aacaaatgag gcatcagtct gaaagccagg gagttggtct gagtgacaag
     5761 gaattggttt cagatgatga agaaagagga acgggcttgg aagaaaataa tcaagaagag
     5821 caaagcatgg attcaaactt aggtgaagca gcatctgggt gtgagagtga aacaagcgtc
     5881 tctgaagact gctcagggct atcctctcag agtgacattt taaccactca gcagagggat
     5941 accatgcaac ataacctgat aaagctccag caggaaatgg ctgaactaga agctgtgtta
     6001 gaacagcatg ggagccagcc ttctaacagc taccctagta taatcagtga ctcttctgcc
     6061 cttgaggacc tgcgaaatcc agaacaaagc acatcagaaa aagcagtatt aacttcacag
     6121 aaaagtagtg aataccctat aagccagaat ccagaaggcc tttctgctga caagtttgag
     6181 gtgtctgcag atagttctac cagtaaaaat aaagaaccag gagtggaaag gtcatcccct
     6241 tctaaatgcc catcattaga tgataggtgg tacatgcaca gttgctctgg gagtcttcag
     6301 aatagaaact acccatctca agaggagctc attaaggttg ttgatgtgga ggagcaacag
     6361 ctggaagagt ctgggccaca cgatttgacg gaaacatctt acttgccaag gcaagatcta
     6421 gagggaaccc cttacctgga atctggaatc agcctcttct ctgatgaccc tgaatctgat
     6481 ccttctgaag acagagcccc agagtcagct cgtgttggca acataccatc ttcaacctct
     6541 gcattgaaag ttccccaatt gaaagttgca gaatctgccc agggtccagc tgctgctcat
     6601 actactgata ctgctgggta taatgcaatg gaagaaagtg tgagcaggga gaagccagaa
     6661 ttgacagctt caacagaaag ggtcaacaaa agaatgtcca tggtggtgtc tggcctgacc
     6721 ccagaagaat ttatgctcgt gtacaagttt gccagaaaac accacatcac tttaactaat
     6781 ctaattactg aagagactac tcatgttgtt atgaaaacag atgctgagtt tgtgtgtgaa
     6841 cggacactga aatattttct aggaattgcg ggaggaaaat gggtagttag ctatttctgg
     6901 gtgacccagt ctattaaaga aagaaaaatg ctgaatgagc atgattttga agtcagagga
     6961 gatgtggtca atggaagaaa ccaccaaggt ccaaagcgag caagagaatc ccaggacaga
     7021 aagatcttca gggggctaga aatctgttgc tatgggccct tcaccaacat gcccacagat
     7081 caactggaat ggatggtaca gctgtgtggt gcttctgtgg tgaaggagct ttcatcattc
     7141 acccttggca caggtgtcca cccaattgtg gttgtgcagc cagatgcctg gacagaggac
     7201 aatggcttcc atgcaattgg gcagatgtgt gaggcacctg tggtgacccg agagtgggtg
     7261 ttggacagtg tagcactcta ccagtgccag gagctggaca cctacctgat accccagatc
     7321 ccccacagcc actactgaac gcgtaaatga ttgcagatcc actagttcta gagctcgctg
     7381 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7441 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7501 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7561 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7621 tgaggcggaa agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc
     7681 ggccccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     7741 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     7801 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     7861 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     7921 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     7981 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     8041 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     8101 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     8161 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     8221 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     8281 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     8341 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     8401 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     8461 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     8521 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     8581 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     8641 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     8701 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     8761 tttcttccat ttcaggtgtc gtgagcgatc gcatcgacag tgtttaaacg ccaccatggt
     8821 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     8881 cgtaaacggc cacaagttca gcgtgagggg cgagggcgag ggcgatgcca ccaacggcaa
     8941 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     9001 gaccaccctg agccacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     9061 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     9121 ggacgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     9181 ccgcatcgag ctgaagggcg tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     9241 ggagtacaac ttcaacagcc acaacatcta tatcatggcc gtcaagcaga agaacggcat
     9301 caaggtgaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca
     9361 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca gccactacct
     9421 gagcacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     9481 ggagttccgc accgccgccg ggatcactct cggcatggac gagctgtaca agtgagaatt
     9541 cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     9601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     9661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     9721 gggcaggaca gcaaggggga ggattgggaa gagaatagca ggcatgcgtt taaacattag
     9781 tcgacagggc cgccactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9841 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9901 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     9961 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    10021 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    10081 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    10141 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    10201 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10261 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10321 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10381 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
    10441 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10501 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10561 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10621 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10681 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10741 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10801 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10861 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10921 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10981 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    11041 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    11101 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    11161 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    11221 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    11281 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    11341 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    11401 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    11461 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11521 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11701 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    12001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    12061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    12121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    12181 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    12241 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    12301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    12361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    12421 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    12481 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    12541 ac
//
