LOCUS       sequence-224976-       12791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a fusion protein of human BRCA1 with the 
            human RNF169 Ubiquitin binding domain and BFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 12791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-BRCA1-RNF169UBD
FEATURES             Location/Qualifiers
     source          1..12791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        38..417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..696
                     /label=chicken beta-actin promoter
     intron          697..1708
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1632..1654
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        1721..1739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7391..7409
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7411..7420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7420..7443
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7605..7621)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7641..7865
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          8086..9024
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8981..9001
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      9059..9068
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9065..9067,9068..9070,9071..9784)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9110..9131)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    9815..9926
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10044..10061)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10044..10060)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10053..10075)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10202..10657
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10289..10308)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10499..10520
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10683..10787
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10788..10856,10857..11648)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11006..11025)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11819..12407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12308..12327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12561..12578
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12695..12716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12731..12736,12737..12754,12755..12761)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12769..12785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(12774..12791,1..5)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 acaggaaaca gctatgacca tgaggcgcgc cggattcgac attgattatt gactagttat
       61 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      121 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      181 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      241 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      301 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      361 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      421 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      481 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      541 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtc gcgccttcgc
      721 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      781 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      841 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      901 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      961 cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1021 gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1081 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     1141 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
     1201 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1261 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1321 cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     1381 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     1441 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg gcgaagcggt
     1501 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1561 cccttctcca tctccagcct cggggctgcc gcagggggac ggctgccttc gggggggacg
     1621 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     1681 tgttcatgcc ttcttctttt tcctacagat ccttaattaa taatacgact cactataggg
     1741 gccgccacca tggatttatc tgctcttcgc gttgaagaag tacaaaatgt cattaatgct
     1801 atgcagaaaa tcttagagtg tcccatctgt ctggagttga tcaaggaacc tgtctccaca
     1861 aagtgtgacc acatattttg caaattttgc atgctgaaac ttctcaacca gaagaaaggg
     1921 ccttcacagt gtcctttatg taagaatgat ataaccaaaa ggagcctaca agaaagtacg
     1981 agatttagtc aacttgttga agagctattg aaaatcattt gtgcttttca gcttgacaca
     2041 ggtttggagt atgcaaacag ctataatttt gcaaaaaagg aaaataactc tcctgaacat
     2101 ctaaaagatg aagtttctat catccaaagt atgggctaca gaaaccgtgc caaaagactt
     2161 ctacagagtg aacccgaaaa tccttccttg caggaaacca gtctcagtgt ccaactctct
     2221 aaccttggaa ctgtgagaac tctgaggaca aagcagcgga tacaacctca aaagacgtct
     2281 gtctacattg aattgggatc tgattcttct gaagataccg ttaataaggc aacttattgc
     2341 agtgtgggag atcaagaatt gttacaaatc acccctcaag gaaccaggga tgaaatcagt
     2401 ttggattctg caaaaaaggc tgcttgtgaa ttttctgaga cggatgtaac aaatactgaa
     2461 catcatcaac ccagtaataa tgatttgaac accactgaga agcgtgcagc tgagaggcat
     2521 ccagaaaagt atcagggtag ttctgtttca aacttgcatg tggagccatg tggcacaaat
     2581 actcatgcca gctcattaca gcatgagaac agcagtttat tactcactaa agacagaatg
     2641 aatgtagaaa aggctgaatt ctgtaataaa agcaaacagc ctggcttagc aaggagccaa
     2701 cataacagat gggctggaag taaggaaaca tgtaatgata ggcggactcc cagcacagaa
     2761 aaaaaggtag atctgaatgc tgatcccctg tgtgagagaa aagaatggaa taagcagaaa
     2821 ctgccatgct cagagaatcc tagagatact gaagatgttc cttggataac actaaatagc
     2881 agcattcaga aagttaatga gtggttttcc agaagtgatg aactgttagg ttctgatgac
     2941 tcacatgatg gggagtctga atcaaatgcc aaagtagctg atgtattgga cgttctaaat
     3001 gaggtagatg aatattctgg ttcttcagag aaaatagact tactggccag tgatcctcat
     3061 gaggctttaa tatgtaaaag tgaaagagtt cactccaaat cagtagagag taatattgaa
     3121 gacaaaatat ttgggaaaac ctatcggaag aaggcaagcc tccccaactt aagccatgta
     3181 actgaaaatc taattatagg agcatttgtt actgagccac agataataca agagcgtccc
     3241 ctcacaaata aattaaagcg taaaaggaga cctacatcag gccttcatcc tgaggatttt
     3301 atcaagaaag cagatttggc agttcaaaag actcctgaaa tgataaatca gggaactaac
     3361 caaacggagc agaatggtca agtgatgaat attactaata gtggtcatga gaataaaaca
     3421 aaaggtgatt ctattcagaa tgagaaaaat cctaacccaa tagaatcact cgaaaaagaa
     3481 tctgctttca aaacgaaagc tgaacctata agcagcagta taagcaatat ggaactcgaa
     3541 ttaaatatcc acaattcaaa agcacctaaa aagaataggc tgaggaggaa gtcttctacc
     3601 aggcatattc atgcgcttga actagtagtc agtagaaatc taagcccacc taattgtact
     3661 gaattgcaaa ttgatagttg ttctagcagt gaagagataa agaaaaaaaa gtacaaccaa
     3721 atgccagtca ggcacagcag aaacctacaa ctcatggaag gtaaagaacc tgcaactgga
     3781 gccaagaaga gtaacaagcc aaatgaacag acaagtaaaa gacatgacag tgatactttc
     3841 ccagagctga agttaacaaa tgcacctggt tcttttacta agtgttcaaa taccagtgaa
     3901 cttaaagaat ttgtcaatcc tagccttcca agagaagaaa aagaagagaa actagaaaca
     3961 gttaaagtgt ctaataatgc tgaagacccc aaagatctca tgttaagtgg agaaagggtt
     4021 ttgcaaactg aaagatctgt agagagtagc agtatttcac tggtacctgg tactgattat
     4081 ggcactcagg aaagtatctc gttactggaa gttagcactc tagggaaggc aaaaacagaa
     4141 ccaaataaat gtgtgagtca gtgtgcagca tttgaaaacc ccaagggact aattcatggt
     4201 tgttccaaag ataatagaaa tgacacagaa ggctttaagt atccattggg acatgaagtt
     4261 aaccacagtc gggaaacaag catagaaatg gaagaaagtg aacttgatgc tcagtatttg
     4321 cagaatacat tcaaggtttc aaagcgccag tcatttgctc tgttttcaaa tccaggaaat
     4381 gcagaagagg aatgtgcaac attctctgcc cactctgggt ccttaaagaa acaaagtcca
     4441 aaagtcactt ttgaatgtga acaaaaggaa gaaaatcaag gaaagaatga gtctaatatc
     4501 aagcctgtac agacagttaa tatcactgca ggctttcctg tggttggtca gaaagataag
     4561 ccagttgata atgccaaatg tagtatcaaa ggaggctcta ggttttgtct atcatctcag
     4621 ttcagaggca acgaaactgg actcattact ccaaataaac atggactttt acaaaaccca
     4681 tatcgtatac caccactttt tcccatcaag tcatttgtta aaactaaatg taagaaaaat
     4741 ctgctagagg aaaactttga ggaacattca atgtcacctg aaagagaaat gggaaatgag
     4801 aacattccaa gtacagtgag cacaattagc cgtaataaca ttagagaaaa tgtttttaaa
     4861 ggagccagct caagcaatat taatgaagta ggttccagta ctaatgaagt gggctccagt
     4921 attaatgaaa taggttccag tgatgaaaac attcaagcag aactaggtag aaacagaggg
     4981 ccaaaattga atgctatgct tagattaggg gttttgcaac ctgaggtcta taaacaaagt
     5041 cttcctggaa gtaattgtaa gcatcctgaa ataaaaaagc aagaatatga agaagtagtt
     5101 cagactgtta atacagattt ctctccatat ctgatttcag ataacttaga acagcctatg
     5161 ggaagtagtc atacatctca ggtttgttct gagacacctg atgacctgtt agatgatggt
     5221 gaaataaagg aagatactag ttttgctgaa aatgacatta aggaaagttc tgctgttttt
     5281 agcaaaagcg tccagagagg agagcttagc aggagtccta gccctttcac ccatacacat
     5341 ttggctcagg gttaccgaag aggggccaag aaattagagt cctcagaaga gaacttatct
     5401 agtgaggatg aagagcttcc ctgcttccaa cacttgttat ttggtaaagt aaacaatata
     5461 ccttctcagt ctactaggca tagcaccgtt gctaccgagt gtctgtctaa gaacacagag
     5521 gagaatttat tatcattgaa gaatagctta aatgactgca gtaaccaggt aatattggca
     5581 aaggcatctc aggaacatca ccttagtgag gaaacaaaat gttctgctag cttgttttct
     5641 tcacagtgca gtgaattgga agacttgact gcaaatacaa acacccagga tcctttcttg
     5701 attggttctt ccaaacaaat gaggcatcag tctgaaagcc agggagttgg tctgagtgac
     5761 aaggaattgg tttcagatga tgaagaaaga ggaacgggct tggaagaaaa taatcaagaa
     5821 gagcaaagca tggattcaaa cttaggtgaa gcagcatctg ggtgtgagag tgaaacaagc
     5881 gtctctgaag actgctcagg gctatcctct cagagtgaca ttttaaccac tcagcagagg
     5941 gataccatgc aacataacct gataaagctc cagcaggaaa tggctgaact agaagctgtg
     6001 ttagaacagc atgggagcca gccttctaac agctacccta gtataatcag tgactcttct
     6061 gcccttgagg acctgcgaaa tccagaacaa agcacatcag aaaaagcagt attaacttca
     6121 cagaaaagta gtgaataccc tataagccag aatccagaag gcctttctgc tgacaagttt
     6181 gaggtgtctg cagatagttc taccagtaaa aataaagaac caggagtgga aaggtcatcc
     6241 ccttctaaat gcccatcatt agatgatagg tggtacatgc acagttgctc tgggagtctt
     6301 cagaatagaa actacccatc tcaagaggag ctcattaagg ttgttgatgt ggaggagcaa
     6361 cagctggaag agtctgggcc acacgatttg acggaaacat cttacttgcc aaggcaagat
     6421 ctagagggaa ccccttacct ggaatctgga atcagcctct tctctgatga ccctgaatct
     6481 gatccttctg aagacagagc cccagagtca gctcgtgttg gcaacatacc atcttcaacc
     6541 tctgcattga aagttcccca attgaaagtt gcagaatctg cccagggtcc agctgctgct
     6601 catactactg atactgctgg gtataatgca atggaagaaa gtgtgagcag ggagaagcca
     6661 gaattgacag cttcaacaga aagggtcaac aaaagaatgt ccatggtggt gtctggcctg
     6721 accccagaag aatttatgct cgtgtacaag tttgccagaa aacaccacat cactttaact
     6781 aatctaatta ctgaagagac tactcatgtt gttatgaaaa cagatgctga gtttgtgtgt
     6841 gaacggacac tgaaatattt tctaggaatt gcgggaggaa aatgggtagt tagctatttc
     6901 tgggtgaccc agtctattaa agaaagaaaa atgctgaatg agcatgattt tgaagtcaga
     6961 ggagatgtgg tcaatggaag aaaccaccaa ggtccaaagc gagcaagaga atcccaggac
     7021 agaaagatct tcagggggct agaaatctgt tgctatgggc ccttcaccaa catgcccaca
     7081 gatcaactgg aatggatggt acagctgtgt ggtgcttctg tggtgaagga gctttcatca
     7141 ttcacccttg gcacaggtgt ccacccaatt gtggttgtgc agccagatgc ctggacagag
     7201 gacaatggct tccatgcaat tgggcagatg tgtgaggcac ctgtggtgac ccgagagtgg
     7261 gtgttggaca gtgtagcact ctaccagtgc caggagctgg acacctacct gataccccag
     7321 atcccccaca gccactacgg aggaggtgga agcggaggag gaggaagcgg aggaggaggt
     7381 agccgattaa taatacgact cactataggg gccaccatgg actacaagga tgacgacgat
     7441 aaaaagcttc agcaagagga agaagaccga cagttggctc tgcagttgca gcgcatgttc
     7501 gacaatgaga ggcggactgt gagccggcga aaaggaagtg tggatcagta tctcctacgg
     7561 tccagcaaca tggccggggc caagtagacg cgtaaatgat tgcagatcca ctagttctag
     7621 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7681 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7741 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     7801 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7861 tatggcttct gaggcggaaa gaaccagctg gggctcgaga tccactagtt ctagcctcga
     7921 ggctagagcg gccccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     7981 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     8041 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     8101 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     8161 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     8221 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     8281 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     8341 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     8401 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     8461 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     8521 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     8581 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     8641 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     8701 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     8761 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     8821 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     8881 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     8941 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     9001 caaagttttt ttcttccatt tcaggtgtcg tgagcgatcg catcgacagt gtttaaacgc
     9061 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     9121 ggacggcgac gtaaacggcc acaagttcag cgtgaggggc gagggcgagg gcgatgccac
     9181 caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     9241 caccctcgtg accaccctga gccacggcgt gcagtgcttc gcccgctacc ccgaccacat
     9301 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     9361 cttcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac
     9421 cctggtgaac cgcatcgagc tgaagggcgt cgacttcaag gaggacggca acatcctggg
     9481 gcacaagctg gagtacaact tcaacagcca caacatctat atcatggccg tcaagcagaa
     9541 gaacggcatc aaggtgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct
     9601 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacag
     9661 ccactacctg agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat
     9721 ggtcctgctg gagttccgca ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     9781 gtgagaattc ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     9841 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9901 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     9961 ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgcgttt
    10021 aaacattagt cgacagggcc gccactggcc gtcgttttac aacgtcgtga ctgggaaaac
    10081 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    10141 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    10201 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    10261 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10321 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10381 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10441 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10501 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10561 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10621 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    10681 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10741 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10801 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10861 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10921 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10981 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    11041 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    11101 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    11161 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11221 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    11281 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11341 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11401 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11461 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11521 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11581 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11641 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11701 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11761 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11821 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11881 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11941 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    12001 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    12061 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    12121 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12181 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    12241 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12301 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12361 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12421 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12481 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12541 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    12601 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12661 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12721 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12781 aacaatttca c
//
