LOCUS       sequence-224977-        8558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a fusion protein of single chain Tet 
            repressor with the human Rad18 Ubiquitin binding domain and BFP 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-TetR-Rad18UBD
FEATURES             Location/Qualifiers
     source          1..8558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        38..417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..696
                     /label=chicken beta-actin promoter
     intron          697..1708
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1632..1654
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        1724..1742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1750..2370
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             2452..3072
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             3073..3093
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        3149..3167
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3169..3178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3178..3201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3372..3388)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3408..3632
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          3853..4791
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4748..4768
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4826..4835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4832..4834,4835..4837,4838..5551)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4877..4898)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    5582..5693
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5811..5828)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5811..5827)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5820..5842)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5969..6424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6056..6075)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6266..6287
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6450..6554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6555..6623,6624..7415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6773..6792)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7586..8174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8075..8094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8328..8345
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8462..8483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8498..8503,8504..8521,8522..8528)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8536..8552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8541..8558,1..5)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 acaggaaaca gctatgacca tgaggcgcgc cggattcgac attgattatt gactagttat
       61 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      121 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      181 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      241 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      301 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      361 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      421 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      481 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      541 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtc gcgccttcgc
      721 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      781 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      841 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      901 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      961 cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1021 gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1081 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     1141 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
     1201 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1261 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1321 cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     1381 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     1441 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg gcgaagcggt
     1501 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1561 cccttctcca tctccagcct cggggctgcc gcagggggac ggctgccttc gggggggacg
     1621 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     1681 tgttcatgcc ttcttctttt tcctacagat ccttaattaa taataatacg actcactata
     1741 ggggccacca tgtccagact ggacaagagc aaagtcatca actctgcctt ggagctcctg
     1801 aatgaagttg gcattgaggg cttgaccacc aggaagctgg cccagaagct gggtgtggag
     1861 cagcctaccc tgtactggca tgtgaagaac aagagggctc tgcttgatgc cctggccatt
     1921 gagatgttgg acaggcacca cacccacttc tgccctctgg aaggggagtc ctggcaggac
     1981 ttcctgagga acaatgccaa gagcttcaga tgtgccttgc tctcccaccg ggatggtgcc
     2041 aaagttcact tgggcaccag gcctacagag aagcagtatg agaccctgga gaaccagctg
     2101 gcattcctgt gccaacaagg cttctccctg gaaaatgcct tgtatgccct ctctgctgtg
     2161 ggccacttca ccttgggctg tgtgctggag gaccaggagc accaagttgc caaggaggag
     2221 agggagaccc ccaccactga ctccatgcca ccactgctgc ggcaagctat tgagttgttt
     2281 gaccaccaag gggctgagcc tgcattcctt tttggcctgg aactgatcat ctgtggcctg
     2341 gaaaagcagc tgaaatgtga gtctggctct cacgtgggag gaggtggaag tggaggtgga
     2401 ggtagcggtg gaggaggtag tggaggtggt ggaagcggag gaggtggtag catgtccaga
     2461 ctggacaaga gcaaagtcat caactctgcc ttggagctcc tgaatgaagt tggcattgag
     2521 ggcttgacca ccaggaagct ggcccagaag ctgggtgtgg agcagcctac cctgtactgg
     2581 catgtgaaga acaagagggc tctgcttgat gccctggcca ttgagatgtt ggacaggcac
     2641 cacacccact tctgccctct ggaaggggag tcctggcagg acttcctgag gaacaatgcc
     2701 aagagcttca gatgtgcctt gctctcccac cgggatggtg ccaaagttca cttgggcacc
     2761 aggcctacag agaagcagta tgagaccctg gagaaccagc tggcattcct gtgccaacaa
     2821 ggcttctccc tggaaaatgc cttgtatgcc ctctctgctg tgggccactt caccttgggc
     2881 tgtgtgctgg aggaccagga gcaccaagtt gccaaggagg agagggagac ccccaccact
     2941 gactccatgc caccactgct gcggcaagct attgagttgt ttgaccacca aggggctgag
     3001 cctgcattcc tttttggcct ggaactgatc atctgtggcc tggaaaagca gctgaaatgt
     3061 gagtctggct ctcctaagaa aaagaggaag gtgcacggag gaggtggaag cggaggagga
     3121 ggaagcggag gaggaggtag ccgattaata atacgactca ctataggggc caccatggac
     3181 tacaaggatg acgacgataa accctcgaca tccactttga aacaagttac taaagtggat
     3241 tgtcctgttt gcggggttaa cattccagaa agtcacatta ataagcattt agacagctgt
     3301 ttatcacgcg aagagaagaa ggaaagcctc agaagttctg ttcacaaaag gtagacgcgt
     3361 aaatgattgc agatccacta gttctagagc tcgctgatca gcctcgactg tgccttctag
     3421 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     3481 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     3541 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     3601 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     3661 ctcgagatcc actagttcta gcctcgaggc tagagcggcc ccggtgccta gagaaggtgg
     3721 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     3781 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     3841 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     3901 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     3961 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     4021 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     4081 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     4141 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     4201 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     4261 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     4321 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     4381 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     4441 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     4501 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     4561 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     4621 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     4681 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     4741 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     4801 gcgatcgcat cgacagtgtt taaacgccac catggtgagc aagggcgagg agctgttcac
     4861 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4921 gaggggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
     4981 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagcc acggcgtgca
     5041 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5101 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcacct acaagacccg
     5161 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcgtcga
     5221 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
     5281 catctatatc atggccgtca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     5341 caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5401 cgacggcccc gtgctgctgc ccgacagcca ctacctgagc acccagtccg tgctgagcaa
     5461 agaccccaac gagaagcgcg atcacatggt cctgctggag ttccgcaccg ccgccgggat
     5521 cactctcggc atggacgagc tgtacaagtg agaattccta gagctcgctg atcagcctcg
     5581 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5641 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5701 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5761 tgggaagaga atagcaggca tgcgtttaaa cattagtcga cagggccgcc actggccgtc
     5821 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5881 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5941 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     6001 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6061 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6121 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6181 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6241 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6301 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6361 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     6421 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6481 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6541 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6601 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6661 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6721 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6781 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6841 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6901 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6961 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7021 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7081 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7141 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7201 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7261 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7321 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7381 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7441 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7501 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7561 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7621 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7681 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7741 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7801 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7861 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7921 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7981 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8041 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8101 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8161 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8221 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8281 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8341 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8401 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8461 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8521 gtatgttgtg tggaattgtg agcggataac aatttcac
//
