LOCUS       sequence-224982-       11478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Supporting plasmid for testing protease circuits.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Gao XJ, Chong LS, Kim MS, Elowitz MB
  TITLE     Programmable protein circuits in living cells.
  JOURNAL   Science. 2018 Sep 21;361(6408):1252-1258. doi: 
            10.1126/science.aat5062.
  PUBMED    30237357
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-09-21- 21"; volume: "361"; 
            issue: "6408"; pages: "1252-1258"
COMMENT     Sequence Label: PB-CMV-TO-EGFRvIII-IRES-nlsChe
FEATURES             Location/Qualifiers
     source          1..11478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1409..1788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1789..1992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1943..1963
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1994..2012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2015..2033
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2043..2067
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(4993..5567,5568..5570,5571..5579)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5160..5177)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5387..5406
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5643..5663
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5685..5694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5691..6404
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(5836..5854)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6104..6123
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6420..6467
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     primer_bind     complement(6485..6502)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6491..6698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6731..6751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6736..7065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6916..7051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6978..6997
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7132..7926
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7186..7205)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7796..7815
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7986..8006)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8048..8169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8085..8104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8139..8158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8932..8966
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9457..9473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9457..9473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9470..9492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9481..9497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9505..9511,9512..9529,9530..9535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9550..9571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9688..9705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9859..10447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9939..9958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10618..11409,11410..11478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtaggcgttt tgcgctgctt
     1381 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     1441 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1501 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1561 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1621 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1681 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1741 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1801 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1861 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     1921 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1981 ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
     2041 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2101 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact tatcgccacc
     2161 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
     2221 gcgagtcggg ctctggagga aaagaaaggt aattatgtgg tgacagatca cggctcgtgc
     2281 gtccgagcct gtggggccga cagctatgag atggaggaag acggcgtccg caagtgtaag
     2341 aagtgcgaag ggccttgccg caaagtgtgt aacggaatag gtattggtga atttaaagac
     2401 tcactctcca taaatgctac gaatattaaa cacttcaaaa actgcacctc catcagtggc
     2461 gatctccaca tcctgccggt ggcatttagg ggtgactcct tcacacatac tcctcctctg
     2521 gatccacagg aactggatat tctgaaaacc gtaaaggaaa tcacagggtt tttgctgatt
     2581 caggcttggc ctgaaaacag gacggacctc catgcctttg agaacctaga aatcatacgc
     2641 ggcaggacca agcaacatgg tcagttttct cttgcagtcg tcagcctgaa cataacatcc
     2701 ttgggattac gctccctcaa ggagataagt gatggagatg tgataatttc aggaaacaaa
     2761 aatttgtgct atgcaaatac aataaactgg aaaaaactgt ttgggacctc cggtcagaaa
     2821 accaaaatta taagcaacag aggtgaaaac agctgcaagg ccacaggcca ggtctgccat
     2881 gccttgtgct cccccgaggg ctgctggggc ccggagccca gggactgcgt ctcttgccgg
     2941 aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc ttctggaggg tgagccaagg
     3001 gagtttgtgg agaactctga gtgcatacag tgccacccag agtgcctgcc tcaggccatg
     3061 aacatcacct gcacaggacg gggaccagac aactgtatcc agtgtgccca ctacattgac
     3121 ggcccccact gcgtcaagac ctgcccggca ggagtcatgg gagaaaacaa caccctggtc
     3181 tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc atccaaactg cacctacgga
     3241 tgcactgggc caggtcttga aggctgtcca acgaatgggc ctaagatccc gtccatcgcc
     3301 actgggatgg tgggggccct cctcttgctg ctggtggtgg ccctggggat cggcctcttc
     3361 atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga ggctgctgca ggagagggag
     3421 cttgtggggc ctcttacacc cagtggagaa gctcccaacc aagctctctt gaggatcttg
     3481 aaggaaactg aattcaaaaa gatcaaagtg ctgggctccg gtgcgttcgg cacggtgtat
     3541 aagggactct ggatcccaga aggtgagaaa gttaaaattc ccgtcgctat caaggaatta
     3601 agagaagcaa catctccgaa agccaacaag gaaatcctcg atgaagccta cgtgatggcc
     3661 agcgtggaca acccccacgt gtgccgcctg ctgggcatct gcctcacctc caccgtgcag
     3721 ctcatcacgc agctcatgcc cttcggctgc ctcctggact atgtccggga acacaaagac
     3781 aatattggct cccagtacct gctcaactgg tgtgtgcaga tcgcaaaggg catgaactac
     3841 ttggaggacc gtcgcttggt gcaccgtgac ctggcagcca ggaacgtact ggtgaaaaca
     3901 ccgcagcatg tcaagatcac agattttggg ctggccaaac tgctgggtgc ggaagagaaa
     3961 gaataccatg cagaaggagg caaagtgcct atcaagtgga tggcattgga atcaatttta
     4021 cacagaatct atacccacca gagtgatgtc tggagctacg gggtgaccgt ttgggagttg
     4081 atgacctttg gatccaagcc atatgacgga atccctgcca gcgagatctc ctccatcctg
     4141 gagaaaggag aacgcctccc tcagccaccc atatgtacca tcgatgtcta catgatcatg
     4201 gtcaagtgct ggatgataga cgcagatagt cgcccaaagt tccgtgagtt gatcatcgaa
     4261 ttctccaaaa tggcccgaga cccccagcgc taccttgtca ttcaggggga tgaaagaatg
     4321 catttgccaa gtcctacaga ctccaacttc taccgtgccc tgatggatga agaagacatg
     4381 gacgacgtgg tggatgccga cgagtacctc atcccacagc agggcttctt cagcagcccc
     4441 tccacgtcac ggactcccct cctgagctct ctgagtgcaa ccagcaacaa ttccaccgtg
     4501 gcttgcattg atagaaatgg gctgcaaagc tgtcccatca aggaagacag cttcttgcag
     4561 cgatacagct cagaccccac aggcgccttg actgaggaca gcatagacga caccttcctc
     4621 ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc ccgctggctc tgtgcagaat
     4681 cctgtctatc acaatcagcc tctgaacccc gcgcccagca gagacccaca ctaccaggac
     4741 ccccacagca ctgcagtggg caaccccgag tatctcaaca ctgtccagcc cacctgtgtc
     4801 aacagcacat tcgacagccc tgcccactgg gcccagaaag gcagccacca aattagcctg
     4861 gacaaccctg actaccagca ggacttcttt cccaaggaag ccaagccaaa tggcatcttt
     4921 aagggctcca cagctgaaaa tgcagaatac ctaagggtcg cgccacaaag cagtgaattt
     4981 attggagcat agcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     5041 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     5101 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     5161 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     5221 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     5281 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     5341 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     5401 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     5461 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     5521 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca
     5581 tggtctctaa aggaggttcg tccgacgacg aagcaacagc ggactcgcag cacgccgcac
     5641 ctcctaagaa gaaaaggaag gtaggggatc cccgggtacc ggtcgccacc atggtgagca
     5701 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5761 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5821 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5881 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5941 ttcccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     6001 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     6061 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     6121 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     6181 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6241 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6301 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6361 gccgccactc caccggcggc atggacgagc tgtacaagtc ttaactcgag ggaggcagcc
     6421 gggaccacat ggtgctgcac gagtacgtga acgccgccgg catcacatag cgaatttaaa
     6481 tcggcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6541 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6601 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6661 ggggaggatt gggaagacaa tagcaggcat gctggggacg cggccgcaat ttaacgcgaa
     6721 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6781 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6841 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6901 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6961 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     7021 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     7081 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     7141 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     7201 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     7261 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     7321 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     7381 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     7441 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     7501 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7561 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7621 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     7681 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7741 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     7801 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7861 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7921 ttctgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7981 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgcg
     8041 ttaactaaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8161 tgtatcttat catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctcat
     8221 ctggtctccc ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca
     8281 aactgggttc ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca
     8341 ggaacgaagt ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc
     8401 cttttttagg gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc
     8461 aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg
     8521 ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc
     8581 cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt
     8641 gggggggggc tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt
     8701 aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa
     8761 aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt
     8821 accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc
     8881 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
     8941 attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt
     9001 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
     9061 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct
     9121 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
     9181 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc
     9241 ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg
     9301 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
     9361 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc
     9421 gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9601 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9661 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10021 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10261 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10501 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10561 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10621 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10681 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10741 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10801 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10861 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10921 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10981 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11041 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11101 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11161 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11221 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11281 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11341 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11401 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11461 gaaatgttga atactcat
//
