LOCUS       sequence-224986-       12184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a combination of proteases and their reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Gao XJ, Chong LS, Kim MS, Elowitz MB
  TITLE     Programmable protein circuits in living cells.
  JOURNAL   Science. 2018 Sep 21;361(6408):1252-1258. doi: 
            10.1126/science.aat5062.
  PUBMED    30237357
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-09-21- 21"; volume: "361"; 
            issue: "6408"; pages: "1252-1258"
COMMENT     Sequence Label: PB-CMV-TO-rapTEV-teHCV-hcTVMV-tvDiTEV-Neo
FEATURES             Location/Qualifiers
     source          1..12184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1409..1788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1789..1992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1943..1963
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1994..2012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2015..2033
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2043..2067
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2158..2167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2569..2838
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             2839..2892
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2893..3213
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3583..3639
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3799..3819
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4429..4482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4948..4959
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5395..5451
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5539..6180
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     CDS             6763..6783
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7126..7173
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     primer_bind     complement(7191..7208)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7197..7404
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7437..7457)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7442..7771
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7622..7757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7684..7703
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7838..8632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7892..7911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8502..8521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8692..8712)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8754..8875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8791..8810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8845..8864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9638..9672
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10163..10179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10163..10179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10176..10198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10187..10203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10211..10217,10218..10235,10236..10241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10256..10277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10394..10411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10565..11153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10645..10664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11324..12115,12116..12184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11947..11966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtaggcgttt tgcgctgctt
     1381 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     1441 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1501 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1561 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1621 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1681 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1741 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1801 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1861 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     1921 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1981 ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
     2041 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2101 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact taagctcgcc
     2161 accatgggag gggaatcctt gttcaagggg ccgcgcgact acaatcccat ctccagtacc
     2221 atttgtcatc ttacgaatga aagtgacggt catacaactt ccctgtacgg catcggtttt
     2281 ggacccttca taattacaaa caagcacctg ttccgccgca acaatgggac tttgcttgta
     2341 caatcactgc acggtgtctt taaagtcaaa aataccacca cgcttcagca gcatttgatt
     2401 gatgggcgag acatgataat aatccgcatg cctaaagact tccctccatt cccgcagaag
     2461 ctcaagttta gagagccgca gcgagaagag aggatctgcc tggtcacgac gaacttccag
     2521 acaagcttgg gtgggtccga actgattcgg gtcgctatac tctggcatga aatgtggcat
     2581 gagggtttgg aagaggcttc cagattgtac tttggcgaaa gaaatgtaaa aggtatgttt
     2641 gaggtcctcg agccgcttca cgcgatgatg gagagaggac ctcaaactct taaagaaacc
     2701 agctttaatc aagcatacgg tcgcgatctc atggaagccc aagaatggtg tcgcaaatac
     2761 atgaaaagtg gtaacgttaa agacctcacg caggcttggg acctttacta tcacgtcttc
     2821 agacgcatca gtaagcagga agggcgggga agcctgttga catgcgggga tgtagaagag
     2881 aatccaggcc cgggcgtgca ggtggaaact attagccctg gagacggacg gacctttccg
     2941 aaaagagggc agacgtgtgt tgtgcattac acaggtatgc tcgaagacgg aaagaaattc
     3001 gatagtagtc gagatcgaaa taagccgttc aagtttatgc ttggaaaaca ggaggtaatc
     3061 cgaggatggg aggagggggt cgcccagatg agtgtcggac agcgagcgaa acttaccatc
     3121 agccctgact atgcttatgg ggcgacggga caccccggaa ttattccacc acatgcaaca
     3181 ctcgtatttg atgtggaact gcttaaactc gagggcggtg gaagcttgaa gtctatgtct
     3241 agtatggtga gcgatactag ctgcacgttc ccttctagcg atgggatctt ctggaaacat
     3301 tggatacaaa ctaaagacgg acaatgcggc tccccccttg tgtctacgcg agatggattc
     3361 atagtgggta ttcacagtgc cagcaacttt acgaacacga acaactattt tacaagcgtt
     3421 cctaagaatt ttatggagtt gctgacaaat caggaagcgc agcagtgggt aagcggttgg
     3481 agactgaatg ccgacagcgt cctttggggg ggccacaaag ttttcatggt gaaacctgag
     3541 gagccgtttc agccagtcaa ggaagcgact caactcatga acgcaacaaa tttttcactt
     3601 ttgaaacagg cgggggacgt agaggagaat cctggcccgg agcaactgga gaagaaattg
     3661 caagcgcttg agaaaaaatt ggcacagttg gaatggaaga accaggcact tgagaaaaaa
     3721 ctcgcccagg gcggatcagg atctggaagc gtagtcctgg tgggccgcct tttgctgtca
     3781 gggtccggtt cttccgccga gaacctttat ttccagagtg gcggaggggg atctggcggt
     3841 gggggcggcg ctccgatcac cgcctacgca caacagacgc gcggcctgct gggttgcatc
     3901 ataacgtctt tgaccgggcg cgacaaaaat caggctgaag gagaggtcca gatcgttagc
     3961 acggcggccc agaccttctt ggccacctgc attaatgggg tatgctggac tgtttatcac
     4021 ggagcgggaa ctcggacaat cgcttcacca aagggccctg taatccaaat gtacacaaac
     4081 gtggataagg atctggttgg ttggcctgct ccgcaaggga cacgcagctt gacaccatgt
     4141 gcttgcggat ctagcgacct gtatctcgtc accaggcacg ctgacgtaat accagttcgc
     4201 cgccgaggcg actcacgggg gagtctgctc agccctaggc cgatttccta tctgaaaggt
     4261 tctagcggag gccctttgct ctgccctgcg ggccatgcgg tgggcatttt cagggcggca
     4321 gtatgcacta gaggcgtagc aaaggcagtc gacttcatcc cggtggaaaa cctggaaacc
     4381 acgatgcgca gccccgtctt cactgataac tcaagcccgc cggcggtgga gggccgaggg
     4441 agcctgctca cctgcggtga tgtggaagag aaccccgggc cgtcaaaggc attgctgaaa
     4501 ggagtgcgcg attttaaccc aatcagtgcg tgcgtgtgcc ttcttgaaaa ctcatcagac
     4561 ggacactcag agcgattgtt cggtatcggg tttggtccct acatcatagc taatcagcac
     4621 ctctttagac gaaacaacgg tgaacttact atcaagacga tgcacggcga gttcaaagta
     4681 aagaatagta cacagctcca aatgaaaccc gttgagggcc gcgacataat cgtaatcaaa
     4741 atggcaaagg atttcccacc ctttccgcag aaattgaagt ttcgacaacc aactattaaa
     4801 gacagagtat gtatggtaag tacaaatttc cagcagggct caggctcctc cgacgagatg
     4861 gaggaatgct ctcagcatct cgggggggga ggatctgcac tcaaaaaaga actccaagct
     4921 aataaaaaag agctggctca actcaagtgg gagctccagg ctttgaaaaa agagctcgct
     4981 caaggagggg gaggctctga tgaaatggag gaatgcagtc aacacttggg tggttctggc
     5041 tcaaaaagcg tgagtagtct tgtgagtgag agttcccata tcgtacacaa ggaggacaca
     5101 tctttctggc agcactggat tacgaccaaa gatggtcaat gcggttcacc cttggtctcc
     5161 ataatagatg gtaatatcct gggtatacat tctctcactc acactactaa cggcagcaat
     5221 tactttgtgg agttcccgga aaagttcgtg gccacttatc tggatgcagc cgatggatgg
     5281 tgcaaaaact ggaagttcaa cgcggataag atcagctggg ggagttttac tcttgttgaa
     5341 gatgcaccag aggatgattt catggctaag aagacggttg ctgcaattat ggatgcaacg
     5401 aatttttcac tgctcaagca ggctggcgat gtagaggaaa atcccggacc ctcaggagtg
     5461 agtgaaacag ttcgcttcca ataccacaag agcggtgctt ggaaacttcc cgtctctttg
     5521 gtaaagggag gaacctcaat gtccaaagga gaagaactgt ttaccggtgt tgtgccaatt
     5581 ttggttgaac tcgatggtga tgtcaacgga cataagttct cagtgagagg cgaaggagaa
     5641 ggtgacgcca ccattggaaa attgactctt aaattcatct gtactactgg taaacttcct
     5701 gtaccatggc cgactctcgt aacaacgctt acgtacggag ttcagtgctt ttcgagatac
     5761 ccagaccata tgaaaagaca tgactttttt aagtcggcta tgcctgaagg ttacgtgcaa
     5821 gaaagaacaa tttcgttcaa agatgatgga aaatataaaa ctagagcagt tgttaaattt
     5881 gaaggagata ctttggttaa ccgcattgaa ctgaaaggaa cagattttaa agaagatggt
     5941 aatattcttg gacacaaact cgaatacaat tttaatagtc ataacgtata catcactgct
     6001 gataagcaaa agaacggaat taaagcgaat ttcacagtac gccataatgt agaagatggc
     6061 agtgttcaac ttgccgacca ttaccaacaa aacaccccta ttggagacgg tccggtactt
     6121 cttcctgata atcactacct ctcaacacaa acagtcctga gcaaagatcc aaatgaaaaa
     6181 ggaggcagcg gatccaccac taacattgcg ccggctctgg acggtgcgtt tcatcgtatc
     6241 acttcttcat cctccctgat gggcctgtgt gacgaaaccg cgactattat ccgtgagatt
     6301 actggctacg accgtgtgat ggtagtacgt ttcgatgaag agggtaatgc cgaaattctg
     6361 tccgaacgtc gtcgtgcgga cctggaagcg ttcctgggta accgctaccc ggcgtctact
     6421 attccgcaga tcgctcgtcg cctgtacgaa cataaccgtg ttcgcctgct ggtagatgtg
     6481 aactatactc cggttccgct acagccgcgc atcagcccgc tgaacggtcg tgatctggat
     6541 atgtccctgt cttgcctgcg ctctatgtcc ccggtccacc agaaatacat gcaggacatg
     6601 ggcgttggcg cgaccctggt ttgctctctg atggtgtctg gtcgtttgtg gggtctgatc
     6661 gcttgccacc actacgaacc gcgcttcgtt ccgttccaca ttcgcgctgc tggcgaagcg
     6721 ctggcggaaa cttgtgcgaa ccgcatcgcg acgctggaga gcgaaaatct ctacttccag
     6781 agtgaattct gtgaacgcga gcagatccac ctggcgggct ccattcagcc gcacggtatc
     6841 ctgctggctg tgaaagagcc agacaacgtg gtgatccagg cttctatcaa cgctgcggag
     6901 ttcctgaaca ccaactctgt tgttggccgt ccgctgcgtg acctgggcgg cgatctgcct
     6961 ttgcagatcc tgccgcacct gaacggcccg ctgcacctgg ctccggtgac cctgcgttgt
     7021 accgtgggtt ctccgccgcg ccgtgtggac tgtaccattc accgtccgtc taacggcggc
     7081 ctgatcgtag aactggaacc agcaaccaag ctcgagggag gcagccggga ccacatggtg
     7141 ctgcacgagt acgtgaacgc cgccggcatc acatagcgaa tttaaatcgg cctcgactgt
     7201 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7261 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7321 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7381 agacaatagc aggcatgctg gggacgcggc cgcaatttaa cgcgaattaa ttctgtggaa
     7441 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7501 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7561 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7681 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7741 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7801 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     7861 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7921 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7981 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     8041 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     8101 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     8161 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     8221 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8281 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8341 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     8401 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8461 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     8521 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     8581 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagtcgacaa
     8641 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8701 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgcgttaa ctaaacttgt
     8761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8881 tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg tctcccttcc
     8941 gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact gggttcttat
     9001 atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct
     9061 aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt tttagggccc
     9121 attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
     9181 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
     9241 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
     9301 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc
     9361 cctgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     9421 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     9481 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     9541 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     9601 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     9661 atctttctag ggttaatcta gctgcatcag gatcatatcg tcgggtcttt tttccggctc
     9721 agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc
     9781 cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt
     9841 gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac
     9901 ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag
     9961 ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac
    10021 tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag
    10081 gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc
    10141 gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    10921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    10981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    11881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    11941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    12001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12181 tcat
//
