LOCUS       sequence-225077-       14206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan neuronal expression of Voltron in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14206)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 14206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: pTol2-elavl3-Voltron
FEATURES             Location/Qualifiers
     source          1..14206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    11163..11297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11187..11206)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11241..11260
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11736..11923,11924..11935)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11969..11985)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11969..11985)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12022..12042)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12022..12040)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12061..12077)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12061..12077)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12074..12096)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12085..12101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12109..12115,12116..12133,12134..12139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12154..12175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12292..12309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12463..13051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12543..12562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13222..14013,14014..14082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13845..13864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14083..14187)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttcccg
      721 ggtgtcgacg aattctcgag ctattcctaa agaccttggg tgaccaaaat cttattttaa
      781 taaataaaac tgtttattaa aacttttttg tttcaaagaa ccatatgtat agtgaaattt
      841 ataaaaatat caatttttaa aaagctggtg tactcattta tgttatgaac tctaaaacca
      901 tatactgact gcaagtgatg atgtatagag tgatgtttac gagtaaacat atttagttgt
      961 atacatccta ctgagcacat tttgatgtat gaaataacat tacaagcttt atccaaatta
     1021 agccatttta aaacactgcc aattgaaaat acaaatcctg gaaaaaatcg tctttagcgc
     1081 agtcatttga gccatcctaa tccgttacct cagaccataa taagaaggga taacactagc
     1141 tgtagcaatg gaacacatct gtttcacaca atcatatctc ctgcgccggt gctaagcaga
     1201 ttcagcgtga tcataacatg ctttccactc ataaatgtaa atttacaatt tgcacatgta
     1261 aaacagacac ttttgagata ttggataaaa aaacaagagt atattgctta gtttcatcca
     1321 ccagtcatcc ccacagcgtt tggaaggcca taaaaagtgt ctaaaatcaa tgatcattga
     1381 aagagcacaa gagagactct tacgctgtaa tgccactggg gacaaaagtg acagtctctt
     1441 aatgggctct tctggagggg ctcctgaaca ttaaaaatta tcagcgaaat taccgaaaga
     1501 gcttcaagca actggcatgc ttgatcctct gcgtcggggc ggtgaatagg tgcttcagat
     1561 gccctcttac ccacgggctg gattcagctg ccccgctacc agcggagacc ccctaatgag
     1621 cctctgcaat taagtttatt catgttaagt gtgaacgggg tgcgtgcgga actgtgggca
     1681 gctaacagac ctgggttctt tgtgccacaa gtgctgcctt tattcggctc acaaagcaga
     1741 aaacaacacc cgcacctata atggcgccct cggctgggtc taagaaacgt ggcgagttga
     1801 cagagcagag tgggcggggt taagacagac tgacagcggg acccatctcc atcctcttat
     1861 taacgcttaa cgagtgcctt cctcatgcaa tattcatcgc cactaatatc atccaagctc
     1921 tgagctgagc tggccactta tgtaaggcaa ttatgtaaaa tatcagacag ggcccacact
     1981 cagaatctga ctggggtaga gacgcgggac gagaaccgag agcaagaact gaaagtgaaa
     2041 gtgaccacta aagggaggag aggacagagg ggcaggatgt gtcaagatta ccagagaaca
     2101 cttggccaga aatgcgcaac cattggagct ctccggatta cccaaaggtt aacgagtttg
     2161 aacgcctctg cccactcgcc catctctgat ggtttcccaa gaactcctca agcaaaatat
     2221 atataattgt gtgtattatg cacagacacg agaaaatgct gtttttctga tctgcattac
     2281 agcacatttg cccgccaacg acaataccac ccactcggta cctcgctgac tcctgatgcc
     2341 tgatacctgc gcggtgactg tctacaatct gcataatcaa gagaagttgt gttgaagacg
     2401 agcgccacac aaccgtttcc acaaggtcac ccaaggccgg tgcagatgta ggtgaggtct
     2461 ccataaacag actgaaataa acacatcctc cgctgggaac aacaaccccc tcacgcctca
     2521 tgcatttcca taagcctaca tgcatctctt ccaacttatg gagactcgca cctaccaaca
     2581 tccgcacaac aaagatatac agagcgcgct ccctcaggtc aaggccctgt gggggtctgt
     2641 gcagaaatag gtcatttgtc acacatcaag tcctggggca ggagatgcat tatagatgag
     2701 accaaacagc ctgtctcggt gagctctacc cactccctga gactagaaat gggggaaggg
     2761 agcttgagat aacaaccgct gcaatcactg tgtcgatgtt taatatcagc accaaccggg
     2821 aacaataagg agatgggtgc attcatgttc acatcttacc agtcaagtat catcgaaccg
     2881 gcttgataac cacacctcgt gtaatagctg agcagatagt tgtcatttta aagcgttggc
     2941 ctttgtcgat tatgtaatgc gcacattcaa cacatggtaa tatagaaacg gttatgtcga
     3001 ggttgttttg tccagagatg accttcacac agttacagcc gctctgcatc cacacaaatg
     3061 gaggacttaa tcgtggactg cattcttaga aatgatctac aaagacaaat aatgtgaaat
     3121 caagaaagga caaaatttaa gtaaggggat gagggagaga gagaacgagg ggcaaggaga
     3181 aagcatggct cctgtctttt tctgcaccca tctgttcgga gtgcaggtgg agctctattc
     3241 actcagctct gcatgtgtgt ttgggggggg caggaagaaa gggagggcaa aaggaagagt
     3301 ggagagatgg tgggggctgg agggatgggg ggttctcggt gatctctcct gaaggggata
     3361 atgggagagc agcgctttgc aatggctgcc atgtagtacc ctccctgcac aattagccaa
     3421 tcagcagcaa gctctgccag ccagaaggac acataaaaga agaacattgc agcagaggca
     3481 cagaaggagc ctgcgaggag ctgggaaata cacacacaac agcagaacca caacaccctc
     3541 ccctggacac accctactgg ggatcactgc ttttcttttt ttctgaacca tcgcccacgc
     3601 cacacggaga gaaatctctc tctcatcatc atcctgaaga aaaccccctt atcctcattt
     3661 tcacactgct gaggaaaacc tacaatcgca cgggctgaga tttcctggcg aagactgtcc
     3721 tttttccttt ttcttttttt ttcttttcct ttggaaactg acatttgcat ttctccattc
     3781 caagccacgg cgtaataata tctgcaatcc agcctgaaga cctgcaaatc gaaggaccta
     3841 gatcactatc atctttgtac gtcaagaatg gttactgtac gtataacctt tctttctttt
     3901 gctctgacca atatgaaaca ctaaaatcga ttcgagcagc ctctcagcat caattacagt
     3961 gcgtgaaaaa cattcaaata gaggcagcaa atattacatg tgaaaataca ggctggctaa
     4021 atccaagcta atttagaaat gtggtcaaaa cgcatactgg cacgtctaat cgcggattca
     4081 gtaaacaaga ttaacgatta gcccagtgta taagtcatat gatacaggca tgcgcgagag
     4141 catcgctaca cccgagctgg cttcattttc ggaggaaaat caaaacattg ctttctcctg
     4201 ccgtgcgaac cattcgtcat aaaccgtaat acgcaacata catttattac tacatccgtt
     4261 aattagcgat aattagccgt tattaacaaa gagcgctgag gaattcctct caaatagcgg
     4321 aggtgcggcg gaggcagagg ggcgtaaaag ggcacatgcg tgcctggctc aaaaaaggat
     4381 gctccagact gaaactcaga gacaaaacac gatgcctcgg aggctgagag cccatcgaga
     4441 ggaaaggcaa agaaaagggt cgagtcactc agagggggag gggagatatt gtgcatccat
     4501 tggtttgaat tgaagcaggc agaataaaaa tccttgcgat tatgcttcgt gagagatcgc
     4561 gagagaggaa aaggctaaat gccacgtatg caaatggata aatgtcatct atttcttcgc
     4621 gagtagacat tttgtggaca acgagagtgt aaataagcga tatgagggga atacaaaggc
     4681 cttgagggaa agcgttttgg gcgtgtcttg tcgaaaagaa gacagcgatg gcacgcgctc
     4741 agaaccacca tcttcacgta gataatccgc gtgaaaacaa taacaaaact gctcatttta
     4801 acacgagcag gtgtttgaaa ccacccaaaa aagattgtct aaaatattaa gattaattaa
     4861 tgacgtttaa atgtccaggc ctgatataaa tgaagcgtgt agtaagagta atcgcctttg
     4921 tttcttttca gagatacgtt ttcagagatt cagggaatta tatcttcaat aaaataatgt
     4981 ttgatttcat tattttaaag ctttttaaga agtaaaaata cacattattc atttacattt
     5041 tattttattt aaaggcctat ggtcttgaaa ataataagtg gaatatatag gctatatatt
     5101 cgtctaagga cagccggtgt tattttatag tgacctcaga caaaagccga gacaaaacga
     5161 ggcgcttgcc tttcatttta atccttaata gacggtgtga tgaatgaatg aatgaatgaa
     5221 taaatgaatg aatgacgggc ccttcggcat gttgttccgc agtcctccgt gagcacgagt
     5281 ctctaccgca gtgccaagca cataaaaccg ttgagtcaga taaccttctc caggcgcgta
     5341 tacacaattc aaggacaagc acatcacaac tatacacaac acaacacaac cgcctaaacg
     5401 cacgagctca tcaatcgcaa tttaaaggca tgtcagtcaa agaaaaaggc ggcgttattt
     5461 aaaaccaatg ttcaacaata tttgtatgct gactagcccg ggcgcgcacg ctataagatc
     5521 acaaggcctt gttttttccc tttattcaaa atatcattaa ttattacagg tcataaaaca
     5581 aaacaatgca aattacaatg atttaaagac tattgattta acaataatca gttaattata
     5641 gaataatatt ataatatttt acatacaaat gaaagatgac attatttgat gacattgtaa
     5701 ttaaaatata tgcaatatat ttttacgttt ttattaatta ggctattact attattataa
     5761 ttatcattat ttttattatt attattatta ttaaatagtc ttgcgaaata agagtgaagg
     5821 cagttgcaat gttcattgtt cccggagagt cccgcagcct ctggcctcaa cacaaagcgc
     5881 tactgtttac attaatattc atagtgctgc catggtctct gtagaggagg aacgggtggc
     5941 tcttttgttc gctagtgtca atacgtttat tcagcaaaaa gcagctcgga tttgtgaatg
     6001 cccaacaagc acacgcgcac tccaatcaaa ggacgagagg ataacgattt taaacgattc
     6061 gcaaacgtgt ttatttgtaa agacgacatc aagagcttaa tgtccacttg aaaagaaata
     6121 aatatgcccg cggcgccaag gatattttga atgtagtcgt tcatggtgag ccactcccga
     6181 attcagcact ctggagagcg accaaagagg aatagcggaa tagcaactac ttgagccatt
     6241 tctctccgtt tgatggcgtt catgattaaa aatataatca cgtgattttt tatacatata
     6301 taaatatata cataaagtaa cgctttcctt ttgagaatac ataaatattt attcgcgtga
     6361 taaatcaatg atcttatatt tatttgcacg accgaaatta gccaaattca ccaattaaaa
     6421 aaaaaataag ccaacaaaaa agaagggttt atccgttcag ttttgacttg tgtcctgttg
     6481 ttttacagct gccactgtga tcccttaagc tgcagtgaga gtgtggctaa tgccttttgt
     6541 ttaagataat cctgtaatct gttaccgaaa cggcctattg acaagccggc attcacattt
     6601 cagtcaggag gcccgtccag acatgtacat ttatgaatta cgaatgataa aattaagatc
     6661 tgcattaggc gtcagaaatg tcacgaacac ccattcattt cccacagcca ataatggatc
     6721 atgctgggaa gcgctatcct cgcagtctca aaattaatgt tgacatgttg cgtagagcta
     6781 cagattagat cagccatatg ctttatgtgt ttacctcagc ggatcttcag caagatgtgt
     6841 ttattttaag aaaatggctt cctcgctgcc tgcacagggg cattaaggaa gtgacgtgag
     6901 cgtcctcaca gtcaaattac tttatctcat gctgctttca ggcctctgct tattttatta
     6961 ttattatttt aaattagggg gaatcacggt ggcgttgtgg gtagggtagc gcgatcacct
     7021 tacagcaaga aggtcgctgg ttcgagctct ggctgggtca gttggcattt ctgtgtggag
     7081 tttaaattga attgaaattg aataaactaa attggcccta gtgtatgtgt gtgaatgtaa
     7141 gtgtgcatgg gtgtttccca gtgttgggtt tgactggaag agcatccgct tcgtaaaaca
     7201 tatgctggat aagttggcgg ttcatttcac tgtggtgacc gctgattaat aaagggacta
     7261 agctgaaaat gaaatgaatt aatacatttt aaatgagctt ttgctgacat gtttatgatt
     7321 acaaatagtg aaattgtatg ttaatttacg ataagtgcat tgttctaaat tcatttttat
     7381 gtagcagctg gtttttcttc catttgtgtc attcaacaga acatttttct aaaaaagtga
     7441 acttaaattt tctcctccct ctgacaaggc ttgatggtaa tttcagccga ctctaattac
     7501 agactcacta aaaggaatcg gtgcagtcat ttatctttat cagcgcgctg gtgggaaggt
     7561 aataggtttt gcttatgagt tgtttgcgtg cgagtctggg tccttgtgta ggcgatgctg
     7621 atgcactgca ttaaatattg agggagaagt cgctcaaaaa tagcaaagta caggctgggg
     7681 caggaacgtc aaagcccgct cagtggccac gtttcttgtt tcgtaagtcc tatttttaat
     7741 agcgataatt caggctcagt agaaggaact ccagcactat aagcagtctc tcgcctgtct
     7801 cggcgagtta tgtattttgg gatgaagtgc tcttccacct ctacctctgc tgtgctggcg
     7861 ccagtatctg ggctgtaaga tagtgtacgt gtcagtctgt gtggccgaag agcctgtgtg
     7921 ttgtgtaact cactacattc gtcgctgtgg atggctgact tgccatgggg aggtttactt
     7981 cagttcacga atcacattcg ccacgatctt gtcactttgt gtcaagactc gctctctgtg
     8041 tgtcaacagc tctgctgttt gaggttgaaa ttctgaactt gagatatatc ccaggatata
     8101 ttagggctac tgccaacgga aaaagcaaat ggtgccatgg ccatttgatt gatcaaacaa
     8161 acaccctttt gcttgggtga cagtgaaatg tcagatccat gtttgtactg ctttaataac
     8221 ttgcctctct gagctttctc cttgtcagtg tcaatttgcc tgagatataa attccggttg
     8281 ccccgaaagc ttattttatt aaaaaaaagc taacatgcac tcatacaacg tgcgcacata
     8341 catgtgaggc ttgctaaaag tcaagaagca agccaatagc aagagtcgtc agactgctgt
     8401 tacctgggca acctggttag aggtaaggct caaggcaaga ataaatcatt ccactgaaga
     8461 gacacagcgt tacagcgaga tgagagcaag aggcgatagt gaagggagaa caaaagaaca
     8521 gcctacccac actagtgcag atctacctgt ttgagctact gattgttcag tttgattaga
     8581 tatcagacaa ttctgctatc tccaattgca ttgctgcatg aattcctaga aatcatttta
     8641 atcaccaaaa actagtaggg acacttgtgg cgtatatgat tctgcctgtt gattgtgggg
     8701 atccaacaga aagatcatga atgcattgtt aattaagtcg gtgagacacc ctgttccacc
     8761 ctaggggccg ttctggggaa agagtgcttt cagtcagcta aagatgacta atatgtgaac
     8821 atatattact acacatgcca atttgtacat tctggatagt taccagccct gggaaaacat
     8881 cgaaaaaaat aaatcaacat agataaaatt ggcaaatccc ttctggtacg atgcagagtg
     8941 cttgctctga gaacttgtgc attgtgacag aaagcgaccg ttaaaaactg aaagtagcat
     9001 ctacaacctg catacatctg ttctttgaaa atcccctgta ggccaaatta agcatcacgc
     9061 tgtgctccac agcaacacgg atgaaccgaa aacccagaca caaacgcaca cacttttcac
     9121 ataaacaagg tttagttatg ctagctatgc tttttcgtcc tccctttgtc atcagcggcg
     9181 agtgacgtaa cacagtttga ccctggacag cgtaaagggg gggttgggaa tgagtgaaag
     9241 gactgtgaca tctgcagggc caggggctga tggagagagc tgaagtgagg gaggtcagag
     9301 gaggaggaag ggtgggatat tgtcctcctc aggccctatc cctctcagac gcattgattt
     9361 ttaacctctc agcgagaatg ccaagcatta cacccctcaa attatggttc ctcacccaac
     9421 ctgttatatt ttccacctgc agataattac cggtgccatc atggctgacg tggaaaccga
     9481 gaccggcatg attgcacagt ggattgtctt tgctattatg gctgctgctg ctattgcttt
     9541 tggagtggct gtgcactttc ggccttcaga gctgaagagc gcatactata tcaacattgc
     9601 catctgcact atcgccgcta ccgcttacta tgcaatggcc gtgaactacc aggacctgac
     9661 aatgaatggt gaaaggcagg tggtctacgc aagatatatt aactgggtgc tgaccacacc
     9721 actgctcctg ctcgatctca tcgtcatgac caagatgggc ggagtgatga tttcttgggt
     9781 catcggcgca gacattttca tgatcgtgtt tggtattctg ggcgccttcg aggatgaaca
     9841 caagttcaaa tgggtgtact ttatcgctgg atgtgtgatg caggcagtcc tgacatacgg
     9901 gatgtataac gccacttgga aagacgatct gaagaaaagc cccgagtacc atagctccta
     9961 tgtcagtctg ctcgtcttcc tgtcaatcct ctgggtgttt tatcctgtcg tgtgggcttt
    10021 cgggtctggt agtggcgtgc tgtccgtcga caatgaggcc attctcatgg gaatcctgga
    10081 tgtgctcgct aagccactgt ttggaatggg gtgcctcatt gcccatgaga ctatcttcaa
    10141 gatcggtact ggctttccat tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca
    10201 ctacgtcgat gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac
    10261 ctcctcctac gtgtggcgca acatcatccc gcatgttgca ccgacccatc gctgcattgc
    10321 tccagacctg atcggtatgg gcaaatccga caaaccagac ctgggttatt tcttcgacga
    10381 ccacgtccgc ttcatggatg ccttcatcga agccctgggt ctggaagagg tcgtcctggt
    10441 cattcacgac tggggctccg ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt
    10501 caaaggtatt gcatttatgg agttcatccg ccctatcccg acctgggacg aatggccaga
    10561 atttgcccgc gagaccttcc aggccttccg caccaccgac gtcggccgca agctgatcat
    10621 cgatcagaac gtttttatcg agggtacgct gccgatgggt gtcgtccgcc cgctgactga
    10681 agtcgagatg gaccattacc gcgagccgtt cctgaatcct gttgaccgcg agccactgtg
    10741 gcgcttccca aacgagctgc caatcgccgg tgagccagcg aacatcgtcg cgctggtcga
    10801 agaatacatg gactggctgc accagtcccc tgtcccgaag ctgctgttct ggggcacccc
    10861 aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa
    10921 ggctgtggac atcggcccgg gtctgaatct gctgcaagaa gacaacccgg acctgatcgg
    10981 cagcgagatc gcgcgctggc tgtcgacgct cgagatttcc ggcgagccaa ccactaagag
    11041 caggatcacc agcgagggcg agtacatccc cctggaccag atcgacatca acgtgttctg
    11101 ctacgagaac gaggtgtaaa accggtggat cctggccgtc gttttactgt accatcgatg
    11161 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
    11221 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
    11281 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
    11341 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
    11401 atcctctaga tcagatctgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt
    11461 ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg
    11521 attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat
    11581 gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg
    11641 aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt
    11701 caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact
    11761 ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa
    11821 ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa
    11881 aataatactt aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct
    11941 tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc cgccaccgcg
    12001 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
    12061 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    12121 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    12181 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    12241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    12301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    12361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    12421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    12481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    12541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    12601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    12661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    12721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    12781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    12841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    12901 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    12961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    13021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    13081 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    13141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    13201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    13261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    13321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    13381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    13441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    13501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    13561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    13621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    13681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    13741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    13801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    13861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    13921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    13981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    14041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    14101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    14161 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtg
//
