LOCUS       sequence-225079-        8635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express GFP-4EBP1-Rheb15(CAAX mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Lawrence RE, Cho KF, Rappold R, Thrun A, Tofaute M, Kim DJ, 
            Moldavski O, Hurley JH, Zoncu R
  TITLE     A nutrient-induced affinity switch controls mTORC1 activation by its
            Rag GTPase-Ragulator lysosomal scaffold.
  JOURNAL   Nat Cell Biol. 2018 Sep;20(9):1052-1063. doi: 
            10.1038/s41556-018-0148-6. Epub 2018 Jul 30.
  PUBMED    30061680
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0148-6"; journalName: "Nat Cell Biol"; date: 
            "2018-09"; volume: "20"; issue: "9"; pages: "1052-1063"
COMMENT     Sequence Label: pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX
FEATURES             Location/Qualifiers
     source          1..8635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..333
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     284..304
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      371..380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(377..379,380..382,383..1093)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(377..379,380..382,383..1093)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(422..443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(683..702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1030..1051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1460..1483
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1717..1834
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1883..2393
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2312..2330
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2415..3014
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2415..2434)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2911..2931
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3142..3375
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3447..3568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3484..3503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3538..3557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3608..3743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3763..3782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3764..3782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3792..3809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3792..3808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3801..3823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3950..4405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4037..4056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4247..4268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4431..4535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4536..4604,4605..5396)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4754..4773)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5567..6155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6056..6075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6309..6326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6443..6464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6479..6484,6485..6502,6503..6509)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6517..6533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6522..6544
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6541..6557
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6541..6557
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6576..6596
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6578..6596
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6624..6850
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6851..7031
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7078..7203
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7696..7929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8114..8158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8456..8476
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
       61 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      121 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      181 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      241 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      301 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      361 cgctaccggt gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      421 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      481 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      541 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      601 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      661 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      721 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      781 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
      841 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      901 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      961 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     1021 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1081 cgagctgtac aagttcgaaa tgtccggggg cagcagctgc agccagaccc caagccgtgc
     1141 aatccctgca actcgccggg tggtgctcgg tgacggagtg cagctccctc caggtgacta
     1201 cagcacgact ccaggcggca cgctcttcag caccaccccg ggaggtacca ggatcatcta
     1261 tgaccggaaa ttcctgatgg agtgtcggaa ctcacctgtg accaaaacac ccccaaggga
     1321 tctgcccacc attccggggg tcaccagccc ttccagtgat gagcccccca tggaagccag
     1381 ccagagccac ctgcgcaata gcccagaaga taagcgggcg ggcggtgaag agtcacagtt
     1441 tgagatggac attgtcgacg actacaaaga cgatgacgac aagggtggtt ctgccggtgg
     1501 ctccggttct ggctccagcg gtggcagctc tggtgcgtcc ggcacgggta ctgcgggtgg
     1561 cactggcagc ggttccggtg gtggttctgg cggcggttct gaaggtggcg gctccgaagg
     1621 cggcggcatg gacggggcag cttcacaagg caagtcttca agctcggtga tgtaggaatt
     1681 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     1741 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     1801 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     1861 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
     1921 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     1981 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     2041 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     2101 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     2161 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     2221 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     2281 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     2341 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     2401 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     2461 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     2521 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     2581 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     2641 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     2701 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     2761 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     2821 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     2881 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     2941 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     3001 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     3061 cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     3121 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3181 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3241 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3301 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3361 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3421 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3481 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3541 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3601 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3661 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3721 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     3781 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3841 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3901 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     3961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4021 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4141 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4261 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4321 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4381 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4441 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4501 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4561 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4621 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4681 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4741 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4801 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4861 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4921 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4981 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5041 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5101 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5161 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5221 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5281 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5341 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5401 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5461 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5521 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5581 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5641 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5701 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5761 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5821 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5881 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5941 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6001 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6061 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6421 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6481 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6541 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6601 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6661 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6721 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     6781 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     6841 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6901 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6961 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7021 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7081 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7141 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7201 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7261 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7321 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7381 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7441 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7501 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7561 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7621 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7681 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7741 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7801 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     7861 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     7921 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     7981 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8041 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8101 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8161 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8221 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8281 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     8341 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     8401 cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct tcaccgaggg
     8461 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     8521 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     8581 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcat
//
